[Effects of Simvastatin on Expression of Multidrug Resistance Gene and Protein of SHI-1 Cells].

OBJECTIVE: To investigate the effects of simvastatin(SIM) and serum free medium(SFM) on the expression of multidrug resistance gene(MDR1) and protein of SHI-1 cells. METHODS: Trypan blue exclusion assay was used to detect the proliferation level and viability of SHI-1 cells after treatment with SIM and culture in SFM; The multi-drug resistant protein p-gp was measured by flow cytometry after culture in SFM for 1 to 3 days and treatment with various concentration of simvastatin. The effect of SFM culture and SIM treatment on the expression of MDR1 trascript was detected by qPCR; ELISA was used to measure the change of cellular cholesterol after culture in SIM and SFM. Chemosensitivity assay was performed after treatment with SIM for SHI-1 cells. RESULTS: Compared with control group, the growth of SHI-1 cells cultured in SFM decreased in a time-dependent manner. The growth-inhibitory effect was markedly increased when SHI-1 cells were treated with SIM and SFM. The mRNA level of MDR1 gene decreased after SIM treatment or/and culture in SFM. P-gp protein was downregulated in SHI-1 cells cultured in SFM or/and treated with SIM. The cellular cholesterol level increased when the cells were cultured in SFM. Total cellular cholesterol level decreased in SHI-1 cells treated with SIM and cultured in SFM. Chemosensitivity assays found that pre-treatment with SIM could increase the cytotoxicity of DNR to SHI-1 cells. CONCLUSION: Culture with SIM and SFM can downregulate the expression of MDR1 gene and p-gp protein in SHI-1 cells. SIM also can enhance the chemotherapeutic sensitivity of SHI-1 cells.

[Comparative investigation of molecules releasing from intra-hollow calcium-alginate capsules using fluorimetry].

In this paper, the authors employ three different types of dye molecules, Nile red, Rhodamine 6G, fluorescein and a fluorescent protein-R-phycoerythrin (R-PE). The Rhodamine 6G is positively charged molecules, fluorescein is negatively charged molecules, and Nile red is neutral molecules. The R-phycoerythrins have either a net positive or negative charge which is balanced at the isoelectric point (4.22). It is negatively charged molecules also under our experimental condition. The Nile red, rhodamine 6G, fluorescein and R-phycoerythrin are trapped into alginate calcium hollow capsule respectively. The diffusion processes of those molecules from calcium alginate capsule to solution are measured based on a fluorescence method. The results indicate that electrical characteristics of encapsulated molecules have effect on their diffusion behaviors. The positively charged rhodamine 6G is well accordance with a model of control release from porous polymer membranes. The neutral molecules not only can be released from porous polymer framework, they also can directly dissolve out through polymer membrane. The electrostatic repulsion between fluorescein and negatively charged calcium alginate membranes will accelerate the molecular motion, which is propitious to molecules directly dissolving out through polymer membrane. Based on Fick's law of diffusion, R-PEs can be releases from porous polymer framework It shows the longest equilibrium time. Comparing neutral molecules, negatively and positively charge molecules show the stronger interaction on electric polymer membrane, which results in that the diffusion coefficients of rhodamine 6G and fluorescein are less than that of neutral molecule Nile red. The consequences obtained here should readily explain analogous control releasing behaviors of other functional molecules.