Degradation of pyrene in contaminated soil by the dielectric barrier discharge combined with the MnFe2O4 catalyst.

In this work, a spinel oxide of MnFe2O4 was used as the dielectric barrier discharge (DBD) catalyst for the remediation of pyrene-contaminated soil. The performances were investigated through tuning voltage, frequency, catalyst dosage, and soil moisture. Under the optimal conditions, such as the voltage of 10.0 kV, the discharge frequency of 1.0 kHz, MnFe2O4 dosage of 0.3 g, air flow rate of 2.0 L min-1, soil humidity of 5.3%, and degradation efficiency of DBD/MnFe2O4 for pyrene could reach 79.26% after 10 min treatment, which was much higher than that of DBD for pyrene of 42.56%. The DBD/MnFe2O4 system also showed the high degradation efficiency of other pollutants including phenanthrene, p-nitrophenol, and p-nitrophenol in contaminated soil. The enhancing mechanism of the DBD/MnFe2O4 system was discussed.

Ras-PI3K pathway promotes osteosarcoma progression via regulating VRK1-mediated H2A phosphorylation at threonine 120.

Background: Ras-PI3K pathway aberrant activation plays an important role in the occurrence and development of osteosarcoma. This study investigated the functions of Ras-PI3K pathway specific activation on histone H2A phosphorylation at threonine 120 (H2AT120ph) in osteosarcoma cells, along with the possible internal molecular mechanisms.Methods: Cell transfection was done to alter RasG12V/Y40C, H2AT120ph and vaccinia-related kinase 1 (VRK1) expression. Then, cell viability, proliferation, migration and cell cycle distribution were assessed, respectively. qRT-PCR was utilized to measure the VRK1 and Ras-PI3K pathway downstream genes (CYR61, IGFBP3, WNT16B, NT5E, GDF15 and CARD16) expression. Chromatin immunoprecipitation (ChIP) was conducted to evaluate the input levels of H2AT120ph and VRK1 in the promoter regions of Ras-PI3K pathway downstream genes.Results: Ras-PI3K specific activation promoted histone H2AT120ph. H2AT120ph participated in the oncogenic functions of Ras-PI3K pathway on osteosarcoma by modulating the transcription of Ras-PI3K-targeted genes. Moreover, VRK1 contributed to the Ras-PI3K specific activation-induced up-regulation of H2AT120ph and osteosarcoma progression. Ras-PI3K pathway-specific activation-induced up-regulation of H2AT120ph was achieved by up-regulation of VRK1.Conclusions: Ras-PI3K pathway activation promoted osteosarcoma progression might be via up-regulating VRK1-mediated H2AT120ph. We proposed that VRK1 and H2AT120ph could be the potential targets for osteosarcoma diagnosis and treatment.HighlightsH2AT120ph is specifically promoted by Ras-PI3K pathway activation.H2AT120ph joins in the oncogenic effects of Ras-PI3K pathway on osteosarcoma.H2AT120ph regulates the transcription of Ras-PI3K-targeted genes.VRK1 takes part in the regulatory function of Ras-PI3K pathway on H2AT120ph.

Ras-ERK1/2 Signaling Promotes The Development Of Osteosarcoma By Regulating H2BK12ac Through CBP.

BACKGROUND: H2BK12ac is an important histone acetylation pattern of H2B, which has been reported in several cancers. However, whether H2BK12ac joins in Ras-ERK1/2 activation-induced osteosarcoma (OS) cell behaviors remain unclear. The study explored this peradventure and revealed the underlying mechanism. METHODS: MG-63 cells were transfected with pEGFP-N1, pEGFP-RasWT and pEGFP-K-RasG12V/T35S, H2BK12ac and ERK1/2 expression levels were analyzed by Western blot. Effects of H2BK12ac on cell viability, migration, colony formation and cell cycle were investigated by MTT, Transwell, soft-agar colony formation and flow cytometry assays. RT-qPCR and ChIP were performed to study the effect of H2BK12ac and CBP on ERK1/2-downstream gene transcriptions. RESULTS: H2BK12ac was specifically down-regulated by Ras-ERK1/2 activation in MG-63 cells. Down-regulated H2BK12ac participated in regulating cell proliferation and migration of MG-63 cells, meanwhile, affected the transcription of ERK1/2-downstream genes. Additionally, silence of HDAC1 up-regulated H2BK12ac expression, and inhibited the promoting effect of Ras-ERK1/2 on MG-63 cells' proliferation, migration and RNA expression levels of ERK1/2-downstream genes. Further, the degradation of CBP mediated by MDM2 was discovered to be linked to Ras-ERK1/2 activation-induced H2BK12ac down-regulation. CONCLUSION: These findings from the study demonstrated that Ras-ERK1/2 signaling could promote the development of OS via regulating H2BK12ac through MDM2-mediated CBP degradation.

Layered material gamma-ZrP supported platinum catalyst for liquid-phase reaction: a highly active and selective catalyst for hydrogenation of the nitro group in para-chloronitrobenzene.

Chemoselective hydrogenation of para-chloronitrobenzene without any dechlorination over Pt/gamma-ZrP catalyst proceeds effectively, which provides a clean and convenient approach to produce useful para-chloroaniline with excellent activity and selectivity.

Catalytic combustion of ethyl acetate on supported copper oxide catalysts.

Total oxidation of ethyl acetate on supported copper oxide catalysts was investigated. The catalysts have been prepared by wet impregnation method and characterized by XRD, TEM and XPS. Among the catalysts with the supports of TiO2, CeO2/TiO2 and CeO2-ZrO2/TiO2, CeO2-ZrO2 solid solutions doped TiO2 supported catalyst gives the highest catalytic activity. Catalyst with the composition of 5 wt.% CuO/10 wt.% CeO2-ZrO2-TiO2 shows the total oxidation of ethyl acetate at about 270 degrees C with the 100% CO2 selectivity. The characterization studies of supported copper oxide catalysts showed that the highly dispersed CuO is one of the active phase which contacts intimately with the support, the action of the interface between the components was not be ignored.