Significance of Liver Zonation in Hepatocellular Carcinoma.

Liver zonation is fundamental to normal liver function, and numerous studies have investigated the microstructure of normal liver lobules. However, only a few studies have explored the zonation signature in hepatocellular carcinoma (HCC). In this study, we investigated the significance of liver zonation in HCC with the help of single-cell RNA sequencing (scRNA-seq) and multicolor immunofluorescence staining. Liver zonation-related genes were extracted from the literature, and a three-gene model was established for HCC prognosis. The model reliability was validated using bulk RNA and single-cell RNA-level data, and the underlying biological mechanism was revealed by a functional enrichment analysis. The results showed that the signaling pathways of high-risk groups were similar to those of perivenous zones in the normal liver, indicating the possible regulating role of hypoxia in HCC zonation. Furthermore, the co-staining results showed that the low-grade tumors lost their zonation features whereas the high-grade tumors lost the expression of zonation-related genes, which supported the results obtained from the sequencing data.

Non-destructive classification of apple bruising time based on visible and near-infrared hyperspectral imaging.

BACKGROUND: Bruising time of apple is one of the most important factors for internal quality assessment. The present study aimed to establish a non-destructive method for the classification of apple bruising time using visible and near-infrared (VNIR) hyperspectral imaging. In this study, VNIR hyperspectral images were obtained and analyzed at seven bruising periods. Moreover, regions of interest (ROIs) were chosen to construct the bruised region classification model, and spectra of bruised regions were collected and resampled based on four different methods. Subsequently, machine learning algorithms were employed and used for dealing with the time classification model of apples. In order to reduce data redundancy and improve the accuracy of the classification model, a tree-based assembling learning model was used to select feature wavelengths, and linear discriminant analysis (LDA) was used to improve the discernibility of data. RESULTS: The results revealed that the random forest (RF) model can precisely locate bruised regions, while the gradient boosting decision tree (GBDT) model can validly classify apple bruising times with 70.59% accuracy. Data of 128 wavebands were compressed to 13 wavebands, providing a high accuracy of 92.86%. CONCLUSION: The results prove that the hyperspectral technique can be used for predicting apple bruising time, which will help to assess the internal quality and safety of apples. © 2018 Society of Chemical Industry.