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Objective To investigate the status of grief counseling among front-line nurses in Wuhan during the coronavirus disease 2019 (COVID-19) epidemic, so as to provide reference for formulating reasonable nursing management strategies. Methods A total of 225 front-line nurses were investigated with a basic information questionnaire and attitudes and skills of grief counseling scales. Results A total of 214 valid questionnaires were collected, with an effective rate of 95.1%. The score of grief counseling attitudes of nurses was 32.80±9.41, which was in the middle level. Other grief counseling skills, communication ability, whether they had received relevant training, and frequency of contacting bereaved family members had significant impacts on nurses' grief counseling attitudes (P0.05). The score of grief counseling skills was 19.24±4.10, which was in the lower middle level. Grief counseling, frequency of contacting bereaved family members, grief counseling training, and education background had significant impacts on nurses' grief counseling skills (P0.05). Conclusion During the COVID-19 epidemic, there have been room for improvement in grief counseling for bereaved family members among nurses in Wuhan. It is suggested to set up a grief counseling nursing group and establish a long-term and sound training framework.
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Objective To investigate the current status of grief counseling of clinicians from five coronavirus disease 2019 (COVID-19) designated hospitals in Wuhan and to provide reference for hospital management. Methods A total of 223 clinicians from designated hospitals were investigated with a basic information questionnaire and attitudes and skills of grief counseling scales. Results A total of 208 valid questionnaires were collected, with an effective rate of 93.3%. The score of grief counseling skills of clinicians from COVID-19 designated hospitals was 19.35±4.75, which was in the middle and lower level. Responsibilities and roles, and frequency of contacting bereaved family members had significant effects on the grief counseling skills (P0.01). While the score of grief counseling attitudes of clinicians was 33.21±9.23, which was in the middle level. Whether they had received relevant training, the communication ability, and other grief counseling skills had significant impacts on their grief counseling attitudes (P0.01). Conclusion The skills and attitudes of grief counseling of clinicians from COVID-19 designated hospitals in Wuhan still need to be improved. The hospital should set up a grief counseling committee, establish a complete grief guidance system and build a long-term training mechanism.
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Objective To develop an HPLC method for simultaneous determination of jasminoidin, baicalin, berberine hydrochloride and emodin in Zhizi Jinhua(ZZJH) Pills. Methods The analysis was performed on a Zorbax Eclipse XDB-C18(4.6 mm×150 mm, 5 µm) with gradient elution by using the mobile phase of acetonitrile-0.2% phosphoric acid solution. The column temperature was maintained at room temperature and the flow rate was 1.0 ml/min. The detection wavelengths were set at 240 nm and 275 nm, respectively. Results The calibration curves were linear within the range of 0.056-0.3360 µg (r=0.9998) for gardenoside, 0.2080.624 µg (r=0.9990) for baicalin, 0.116-0.696 µg (r=0.9994) for berberine hydrochloride, and 0.040-0.240 µg (r=0.9993) for emodin, respectively. The recoveries of gardenoside, baicalin, berberine hydrochloride and emodin were 91.18 %(RSD=1.60%)z 91.55%(RSD=0.75%)/91.19%(RSD=1.08%) and 92.29%(RSD=1.98%), respectively. Conclusion The method is convenient, fast, sensitive and reproducible, with good precision, specificity and accuracy. So it can be used for simultaneous determination of jasminoidin, baicalin, berberine hydrochloride and emodin in ZZJH Pills.
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<p><b>OBJECTIVE</b>To clarify the expression and clinical significance of metastasis-associated in colon cancer 1 (MACC1) mRNA in hepatocellular carcinoma (HCC).</p><p><b>METHODS</b>The expression and distribution of MACC1 were assessed by quantitative real-time polymerase chain reaction (RT-PCR) and immunohistochemical staining (IHC) in a cohort of hepatitis B virus-related HCC, including 138 in early (A), 96 in intermediate (B) and 120 in advanced stages (C). The association of MACC1 mRNA with disease progression and outcomes was analyzed by univariate and multivariate Cox analysis.</p><p><b>RESULTS</b>The intratumoral expressions of MACC1 mRNA in HCC stage I (0.001 76, range: 0.000 54 - 0.002 47), stage II (0.002 49, range: 0.000 55 - 0.006 78) and stage III (0.008 35, range: 0.006 86 - 0.009 88) were about 3-, 4- and 14-fold higher than that in the normal liver tissue (0.000 59, range: 0.000 57 - 0.000 60), respectively. Intratumoral expression of MACC1 mRNA increased with disease progression from stage I to stage III. HCC clinical staging classification, age, portal vein invasion and tumor differentiation were significantly associated with intratumoral high expression of MACC1 mRNA (All P < 0.05). Immunohistochemical staining showed that there was an increased MACC1 expression in cytoplasm of HCC cells and positive nuclear staining in some cases. Increased MACC1 mRNA expression could predict poor outcome and recurrence in stage A and B HCC postoperatively. The median tumor-free survival and total survival of patients with high MACC1 mRNA expression were 34.0 and 40 months, respectively, significantly lower than that in those with low expression (48.0 and 48.0 months) (all P < 0.01). Cox analysis showed that Child-Pugh grading and high expression of MACC1 mRNA were independent predictive factors, and high expression of MACC1 was an independent predictive factor affecting the tumor-free survival.</p><p><b>CONCLUSIONS</b>MACC1 mRNA up-regulation is a feature of disease progression in HCC. MACC1 mRNA expression in the HCC may become an independent predictive factor for recurrence and survival in postoperative HCC patients.</p>
