RESUMO
@#AIM: To research the correlation between the concentrations of interleukin-6(IL-6)and monocyte chemokine-1(MCP-1)in aqueous humor and the intravitreal ranibizumab injection was injected into the glass body cavity.<p>METHODS: Forty patients(40 eyes)diagnosed with RVO macular edema were selected as the treatment group, twenty patients(20 eyes)underwent cataract surgery were selected as the control group, anterior aqueous humor was collected before surgery in the treatment group and control group. Using the cytometric bead array methods detection the concentration of MCP-1 and IL-6. Comparison and analysis the concentration of MCP-1 and IL-6 before the operation treatment group and control group, comparison and analysis the concentration of MCP-1 and IL-6, BCVA, CMT before and after the operation in the treatment group. <p>RESULTS: The concentration of MCP-1(49.84±16.16、547.75±108.45pg/mL)and IL-6 in the treatment group was higher than the control group(10.71±7.26、235.65±34.45pg/mL,all <i>P</i><0.001)before the operation. There was a positive correlation between the concentration of MCP-1, IL-6 and CMT before the operation in the treatment group(<i>r</i>=0.646, 0.912, all <i>P</i><0.001). The concentration of IL-6 was significantly correlated with MCP-1(<i>r</i>=0.902, <i>P</i><0.001). The treatment group underwent the intravitreal ranibizumab injection was injected into the glass body cavity, the concentration of IL-6, MCP-1(20.08±11.56、228.35±70.69pg/mL)was lower than before. BCVA was improved significantly compared with before operation, CMT decreased after surgery compared to before surgery(<i>P</i><0.001).<p>CONCLUSION: There was a positive correlation between the concentration of MCP-1, IL-6 and CMT, with IVR treatment of secondary macular edema to RVO, to reduce the concentration of MCP-1 and IL-6, to reduce CMT, and reduce macular edema, improved patient's vision level.
RESUMO
The histopathological changes of the second instar nymph of the mealybug Phenacoccus fraxinus infected with Lecanicillium lecanii strain 3.4505 were investigated using light, scanning and transmission electron microscopy. The results demonstrated that L. lecanii 3.4505 could infect P. fraxinus in a short period. At 24 h post-inoculation, the conidia of L. lecanii 3.4505 adhered to the indented gloves or intersegmental folds of the insect body surface. Subsequently, the germinated conidia produced germ-tubes, appressoria and extended hyphae, which tightly adhered to the cuticle. Penetration of cuticle could be achieved either by peg form appressoria or directly by hyphae. Also, the conidia and hyphae could secrete massive mucilages causing visible damage to the host cuticle. After 48 h, the body wall, tissues and organs, including cuticle, trachea, fat body, muscle, Malpighian tubules and nerve ganglion, were destroyed by ramification of hyphae as a result of infection. The endoplasmic reticulum hypertrophied and formed obvious fingerprint agglomerates, and the mitochondria swelled and deformed in the haemocytes. Finally, the mycelium fully occupied the entire haemocoel. The entire bodies were wrapped in a white mycelium, with the mycelium extending radically outward.
