RESUMO
<p><b>OBJECTIVE</b>To establish a quantitative method for calceolarioside B in Caulis Akebiae.</p><p><b>METHOD</b>The samples were separated at 35 degrees C on a Phenomenex Luna C18 column eluted with methanol-water-phosphoric acid (35: 65: 0.05) as mobile phase. Flow rate was at 1.0 mL x min(-1) and the detection wavelength was at 330 nm.</p><p><b>RESULT</b>The calibration curve was linear over the range from 0. 04 to 0. 86 g (r = 0. 9998) and the average recovery was 97.2%. 39 Crude drug materials collected from different areas were determined and the contents of calceolarioside B in Caulis Akebiae were fluctuated from 0. 021% to 0. 745%.</p><p><b>CONCLUSION</b>The method is simple and repeatable and could be used for the quality control of Caulis Akebiae.</p>
Assuntos
Ácidos Cafeicos , Química , China , Cromatografia Líquida de Alta Pressão , Métodos , Ecossistema , Glucosídeos , Química , Magnoliopsida , Química , Classificação , Folhas de Planta , Química , Raízes de Plantas , Química , Caules de Planta , Química , Plantas Medicinais , Química , Classificação , Reprodutibilidade dos TestesRESUMO
<p><b>OBJECTIVE</b>To establish a new quality evaluation method for Chinese herbal medicine, using one chemical reference substance to calculate multi-components simultaneously.</p><p><b>METHOD</b>Relative correction factors (RCF) of mutongsaponin B and C to saponin PJ1, a characteristic component as marker, were calcalated in The chromatographic conditions for determination of the three saponins in Caulis Akebiae akebiae as a research model. The contents of saponin PJ1 were determined by external standard method, and those of mutongsaponin B and C were calculated by saponin PJ1 and their RCF. The accuracy of the new method was evaluated by comparing the calculated contents with the determined contents.</p><p><b>RESULT</b>The analysis procedures were validated. There has been no significant difference between the calculated contents and the determined contents, according to the angle cosine value, and the calcalated RCF have confidence value.</p><p><b>CONCLUSION</b>The method of multi-components quantitation by one marker has been verified in caulis akebiae and it is to be a new quality evaluation pattern for Chinese herbal medicines.</p>
