Normalized spectral settings in light- and myopia-related experimental studies / 中华实验眼科杂志

There are more and more studies investigating the influence of spectral difference on myopia, but the settings of light sources are different in each study.As a physical quantity, light can be measured radiometrically, mainly to detect the energy properties of light.As a psychological and physical quantity, the light should be measured photometrically to detect the degree of light perception of the organism.Different optical measuring tools measure based on the response curve of the photoelectric conversion device.The essence of the light source spectrum setting in the experiment is to control the sensitivity of organisms to light.Therefore, special attention should be paid to the influence of light source setting differences when interpreting similar experimental results.When setting the light in animal experiments, it is necessary to consider the differences in the absorption curve of the animal's photosensitive pigment and the curve of the animal's spectral light efficiency.It is also suggested that a radiometer should be used to unify the photon flux density consistent among different groups based on 200-300 lx light intensity.In this paper, the normalization of spectral settings in experiments related to light and myopia was reviewed.

Study on the Anti-inflammatory,Analgesic and Anti-fatigue Effects of Polysaccharides from Acanthopanax trifoliatus in Rats and Mice / 中国药房

OBJECTIVE:To study anti-inflammatory,analgesic and anti-fatigue effects of polysaccharides from Acanthopanan trifoliatus (ATMP) in rats and mice. METHODS:In hot plate experiment,150 mice were randomly divided into normal control group (constant volume of normal saline),aspirin group [200 mg/(kg·d)],and ATMP high-dose,medium-dose and low-dose groups [400,200,100 mg(gross polysaccharide)/(kg·d)];the threshold of pain was determined,and analgesic effect of ATMP was investigated. 150 mice were included in exhaustive swimming test and then randomly divided into normal control group(con-stant volume of normal saline),Chongcao yangshen jijing group [400 mg/(kg·d)by total saponins],and ATMP high-dose,medi-um-dose and low-dose groups [200,100,50 mg(gross polysaccharide)/(kg·d)];the body weight and exhaustive swimming time of mice were determined,and biochemical process was used to determine the contents of hepatic glycogen and muscle glycogen, and serum levels of BUN,LDH and CK in mice. The anti-fatigue effect of ATMP was investigated. In carrageenan-induced paw swelling experiment,40 rats were divided into normal control group (constant volume of normal saline),dexamethasone acetate group [5 mg/(kg·d)],ATMP high-dose,medium-dose and low-dose groups [100,50,25 mg(gross polysaccharide)/(kg·d)];the degree of paw swelling was recorded,and anti-inflammatory of ATMP was investigated. RESULTS:Compared with normal control group,the threshold of pain in mice were increased in ATMP 400,200,100 mg(gross polysaccharide)/(kg·d)groups;the exhaus-tive swimming time of mice were prolonged significantly,and the contents of hepatic glycogen in mice were increased significant-ly,while serum contents of CK decreased in ATMP 200,100 mg(gross polysaccharide)/(kg·d)groups;the content of muscle gly-cogen in mice was increased significantly in ATMP 200 mg (gross polysaccharide)/(kg·d) group,while serum contents of BUN and LDH were decreased;the degree of paw swelling in rats was decreased in ATMP 100,50,25 mg(gross polysaccharide)/(kg·d) groups,with statistical significance (P<0.01 or P<0.05). There was no significant difference in body weight of mice before and after medication. CONCLUSIONS:ATMP has significant analgesic and anti-fatigue effects on mice and anti-inflammatory effect on rats.

Whole-tumor-antigen-pulsed dendritic cells elicit cytotoxic T-cell response against pediatric nasopharyngeal carcinoma in vitro.

Nasopharyngeal carcinoma (NPC) is endemic in Southeast Asia. Although dendritic cell (DC)-based vaccine has emerged as a promising immunotherapy for various malignancies, its use in pediatric nasopharyngeal carcinoma (PNPC) has not been addressed. In this study, DCs isolated from peripheral blood monocytes of three pediatric patients with advanced (stage IV) NPC were incubated with whole-tumor-antigen preparations and differentiated into immature DCs in the presence of granulocyte-macrophage colony-stimulating factor and interleukin-4, and then underwent maturation when exposed to tumor necrosis factor-alpha. Upon maturation, DCs acquired the ability to stimulate T-cell proliferation as examined by [(3)H]-thymidine incorporation and the ability of these T-cells to secrete interferon-gamma as determined by enzyme-linked immunosorbent spot assay. Cytotoxic assay revealed that mature tumor-antigen-pulsed DCs induced cytotoxic activity of the T-cells against both autologous and allogeneic NPC tumor cells (including NPC tumor cells from a different individual or from CNE-2Z, a poorly differentiated human NPC cell line). Blocking HLA class I molecules by W6/32 inhibited T-cell-mediated cytotoxic activity in both autologous and allogeneic settings. Our results indicate that DCs pulsed with whole-tumor-antigen can effectively activate HLA class I-restricted cytotoxic T-cells in vitro, and thus provide experimental basis for their future clinical use in PNPC.

Health Risk Assessment of Soil-oral Exposure of Heavy Metal Contaminated Soil by in vitro Method / 环境与健康杂志

Objective To assess the health risk of soil-mouth exposure of soil heavy metal pollution by in vitro method. Methods Collected the soil sampling from the heavy metals contaminated field and roadside. Digested the soil sample by in vitro device and determined the content of Cd, Pb, Zn in the solution in the simulated stomach phase and intestine phase respectively with ICP-MS after centrifuging. Calculated the exposure and assess health risk. Results Bioaccessibility of Cd, Pb and Zn at the stomach phase was 21%-33%, 2%-28%, 33%-43% respectively in the polluted areas, and 27%, 23%, 95% in the control area; At the intestine phase was 16%-19%, 3.4%-4.9%, 8%-14% in polluted areas and 11%,2.6%,18% in control area respectively. The amount of available Cd, Pb and Zn in the stomach phase was 0.04-1.46 ?g/d, 3.09-5.53 ?g/d, 3.86-9.39 ?g/d in polluted areas and 0.01 ?g/d, 0.74 ?g/d ,5.64 ?g/d in control area for children, and 0.01-0.36 ?g/d, 0.77-3.88 ?g/d, 0.97-9.85 ?g/d in polluted areas and 0.00 ?g/d. 0.19 ?g/d, 1.41 ?g/d in the control area for adult ; at intestine phase the amount of available Cd, Pb and Zn was 0.03-0.71 ?g/d, 0.50-9.92 ?g/d, 1.26-9.55 ?g/d in polluted areas and 0.00 ?g/d, 0.08 ?g/d, 1.07 ?g/d in the control area respectively for children, 0.01-0.18 ?g/d, 0.11-2.48 ?g/d, 0.31-2.39 ?g/d in the polluted areas and 0.00 ?g/d,0.02?g/d,0.27 ?g/d in the control area respectively for adult. Conclusion Heavy metals exposure through soil-mouth pathway will increase the total health risk of heavy metal to exposed population.

Study on Health Effects and Contributing Factors of Cold and Humid Environment of Residence in Rural Areas / 环境与健康杂志

Objective To study the health effects and contributing factors of cold and humid residence in rural areas with cold climate. Methods 213 rooms of 125 families in rural areas of Huinan county,Jilin province were observed.The microclimate,the temperatures on the surface of inside and outside walls,the condition of heating and heat insulation of 58 rooms among 213 rooms were determined according to the various condition of the cold and humid environment.The health questionnaire,physical examination,determination of skin temperature and tachogram of finger,test of picking up beads were carried out among residents with residential history of more than 5 years. Results The average skin temperature of bodies and fingers and total amount of beads picked up among the observed residents were significantly lower than those of control group.Significant positive correlation was found between the skin temperatures of fingers of observed residents and their indoor air temperatures( t=0 7839,P