Effect of Non-Starch Polysaccharide (NSP) of Wheat and Barley Supplemented with Exogenous Enzyme Blend on Growth Performance, Gut Microbial, Pancreatic Enzyme Activities, Expression of Glucose Transporter (SGLT1) and Mucin Producer (MUC2) Genes of Broiler Chickens

The experiment was carried out in a completely randomized design (CRD) with 625 broiler chicks (Ross 308) for 5 repetitions (25 birds per each replicated) on the 5 treatments diet. Treatments included two different types of cereal grains (wheat, and barley) with or without an enzyme supplementation along with a corn-based diet as control group. The experimental diets were formulated to have similar contents of crude protein, metabolizable energy, total non-starch polysaccharides (NSP) and were fed in two periods of starter and grower. Experimental traits were consisted growth performance, ileal flora numeration, villus morphology in 3 parts of the intestine, digesta viscosity and pancreatic enzyme activity, and determining the gene expression level of glucose transporter (SGLT1) and mucin producer (MUC2) in the jejunum. Results indicated that inclusion of wheat and barley to corn-soy based diet with or without exo-enzymes blend on growth performance traits were significant (p 0.01). Feed intake and average daily gain in wheat diet was lower, conversely FCR was higher than other groups (p 0.01). Maximum microbial count were observed in wheat and barley diets and minimum were observed in enzyme supplemented diets respectively (p 0.01). Control group and enzyme supplemented diets had minimum counting of gram negative, coliform and clostridium, but maximum counting of lactobacilli and bifidobacter were observed in enzyme supplemented diets (p 0.01). Viscosity and activities of pancreatic a-amylase and lipase were significantly increased in chicks fed wheat and barley when compared to the control group fed on corn (p 0.01). Feeding wheat and barley diets reduced villus height in different parts of the small intestine when compared to those fed on a corn diet (p 0.01).

Effect of Non-Starch Polysaccharide (NSP) of Wheat and Barley Supplemented with Exogenous Enzyme Blend on Growth Performance, Gut Microbial, Pancreatic Enzyme Activities, Expression of Glucose Transporter (SGLT1) and Mucin Producer (MUC2) Genes of Broiler Chickens

The experiment was carried out in a completely randomized design (CRD) with 625 broiler chicks (Ross 308) for 5 repetitions (25 birds per each replicated) on the 5 treatments diet. Treatments included two different types of cereal grains (wheat, and barley) with or without an enzyme supplementation along with a corn-based diet as control group. The experimental diets were formulated to have similar contents of crude protein, metabolizable energy, total non-starch polysaccharides (NSP) and were fed in two periods of starter and grower. Experimental traits were consisted growth performance, ileal flora numeration, villus morphology in 3 parts of the intestine, digesta viscosity and pancreatic enzyme activity, and determining the gene expression level of glucose transporter (SGLT1) and mucin producer (MUC2) in the jejunum. Results indicated that inclusion of wheat and barley to corn-soy based diet with or without exo-enzymes blend on growth performance traits were significant (p 0.01). Feed intake and average daily gain in wheat diet was lower, conversely FCR was higher than other groups (p 0.01). Maximum microbial count were observed in wheat and barley diets and minimum were observed in enzyme supplemented diets respectively (p 0.01). Control group and enzyme supplemented diets had minimum counting of gram negative, coliform and clostridium, but maximum counting of lactobacilli and bifidobacter were observed in enzyme supplemented diets (p 0.01). Viscosity and activities of pancreatic a-amylase and lipase were significantly increased in chicks fed wheat and barley when compared to the control group fed on corn (p 0.01). Feeding wheat and barley diets reduced villus height in different parts of the small intestine when compared to those fed on a corn diet (p 0.01).(AU)

The Efficiency of AMEn and TMEn Utilization for NE in Broiler Diets

The experiment was conducted to evaluate the feed intake, body weight gain, total energy (kJ), protein (g) intake, energy and protein efficiency ratio (EER, PER), net energy, and metabolic body weight, on two commercial broiler chickens (Arian and Ross 308 strains). Four treatmentsincluded diets formulated based onAMEn(kJ/g) Total Amino Acid (TAA) (T1), AMEn(kJ/g) Digestible Amino Acid (T2), TMEn (kJ/g) Total Amino Acid (T3), and TMEn (kJ/g) Digestible Amino Acid (DAA) (T4) for commercial broilers chickens. The findings of the study indicated that AMEn or TMEn treatments yielded improved utilization of net energy in 42 days, but did not affect the efficiency of dietary AMEn and TMEn for net energy, diet energy and protein efficiency ratio (EER, PER) in broilers. Findings indicated that NE is a better predictor of poultry feeds than AMEn or TMEn. This is attributed to the fact that AMEn had a significant effect on NE and efficiency of AMEn or TMEn and HI (Heat Increment) for NE value. Finally, NE is the final objective of energy evaluation of feed and feedstuffs for poultry nutrition.

The Efficiency of AMEn and TMEn Utilization for NE in Broiler Diets

The experiment was conducted to evaluate the feed intake, body weight gain, total energy (kJ), protein (g) intake, energy and protein efficiency ratio (EER, PER), net energy, and metabolic body weight, on two commercial broiler chickens (Arian and Ross 308 strains). Four treatmentsincluded diets formulated based onAMEn(kJ/g) Total Amino Acid (TAA) (T1), AMEn(kJ/g) Digestible Amino Acid (T2), TMEn (kJ/g) Total Amino Acid (T3), and TMEn (kJ/g) Digestible Amino Acid (DAA) (T4) for commercial broilers chickens. The findings of the study indicated that AMEn or TMEn treatments yielded improved utilization of net energy in 42 days, but did not affect the efficiency of dietary AMEn and TMEn for net energy, diet energy and protein efficiency ratio (EER, PER) in broilers. Findings indicated that NE is a better predictor of poultry feeds than AMEn or TMEn. This is attributed to the fact that AMEn had a significant effect on NE and efficiency of AMEn or TMEn and HI (Heat Increment) for NE value. Finally, NE is the final objective of energy evaluation of feed and feedstuffs for poultry nutrition.(AU)