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Objective:To investigate the vasorelaxant effect of the isoflavones in red clover on rat mesenteric artery rings. Meth-ods:The rat mesenteric artery rings were placed between two L form metal needles. When stable precontractions induced by vasocon-strictor were got, the different concentrations of isoflavones in red clover and ( or) blocker were added to the baths. The isometric ten-sion of the rat mesenteric artery rings was recorded using a myograph system. Results:The isoflavones in red clover could concentra-tion-dependently relax the rat mesenteric artery rings pre-contracted by K+krebs solution (60 mmol·L-1K+), and compared with the control group (Dmso), the difference was statistically significant (P<0. 01). The isoflavones in red clover-induced relaxation was re-duced by the removal of endothelium, and compared with the complete endothelial group, the difference was statistically significant ( P<0. 01). Pretreatment with Nω-nitro-L-arginine-methylesterhydrochloride (L-NAME,10 -4 mol·L-1) could suppress the relaxing re-sponse to the isoflavones in red clover, and compared with the control group, the difference was statistically significant (P<0. 01). However, indomethacin(10 -5 mol·L-1) did not significantly affect the relaxation induced by the isoflavones in red clover (P >0. 05). Conclusion:The vasorelaxant effect of the isoflavones in red clover is through promoting nitric oxide release from vascular en-dothelium.
RESUMO
Objective:To investigate the vasorelaxant effect of the isoflavones in red clover on rat mesenteric artery rings. Meth-ods:The rat mesenteric artery rings were placed between two L form metal needles. When stable precontractions induced by vasocon-strictor were got, the different concentrations of isoflavones in red clover and ( or) blocker were added to the baths. The isometric ten-sion of the rat mesenteric artery rings was recorded using a myograph system. Results:The isoflavones in red clover could concentra-tion-dependently relax the rat mesenteric artery rings pre-contracted by K+krebs solution (60 mmol·L-1K+), and compared with the control group (Dmso), the difference was statistically significant (P<0. 01). The isoflavones in red clover-induced relaxation was re-duced by the removal of endothelium, and compared with the complete endothelial group, the difference was statistically significant ( P<0. 01). Pretreatment with Nω-nitro-L-arginine-methylesterhydrochloride (L-NAME,10 -4 mol·L-1) could suppress the relaxing re-sponse to the isoflavones in red clover, and compared with the control group, the difference was statistically significant (P<0. 01). However, indomethacin(10 -5 mol·L-1) did not significantly affect the relaxation induced by the isoflavones in red clover (P >0. 05). Conclusion:The vasorelaxant effect of the isoflavones in red clover is through promoting nitric oxide release from vascular en-dothelium.
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10.3969/j.issn.1000-8179.2013.12.004
RESUMO
OBJECTIVE To evaluate risk factors of the death among lung cancer patients with nosocomial pneumonia.METHODS A total of 312 lung cancer patients with nosocomial pneumonia in our hospital were retrospectively analyzed.RESULTS Eighty-seven patients died and remaining 225 patients were cured finally.Risk factors by multivariate analysis were more than 62 years old(OR 1.75,95%CI 1.06-2.98),metastatic disease(OR 9.24,95%CI 5.21-16.17),bacteria/fungi infection(OR 2.31,95%CI 1.15-4.67),and lower serum albumin concentration(OR 1.87,95%CI 1.08-3.13).CONCLUSIONS Older,metastatic diseases,double infections and lower serum albumin concentration are the risk factors correlated with the mortality among lung cancer patients with nosocomial pneumonia.
