RESUMO
The flesh fly Sarcophaga similis show a clear photoperiodic response; they develop into adults under long days, whereas they arrest their development at the pupal stage under short days. Although the involvement of a circadian clock in photoperiodic time measurement is suggested in this species, the anatomical location of the clock neurons responsible for the time measurement has been unknown. We detected two PERIOD-immunoreactive cell clusters in the larval brain; one cluster was located at the dorsoanterior region and the other at the medial region. We further investigated their temporal changes in PERIOD-immunoreactivity and compared their patterns under different photoperiods.
Assuntos
Encéfalo/imunologia , Relógios Circadianos/fisiologia , Ritmo Circadiano/fisiologia , Proteínas de Insetos/imunologia , Neurônios/imunologia , Proteínas Circadianas Period/imunologia , Animais , Comportamento Animal/fisiologia , Larva , Fotoperíodo , SarcofagídeosRESUMO
Interstitial lung disease and scleroderma renal crisis are major complications of systemic sclerosis, which occasionally coexist in patients with the diffuse cutaneous subtype. We herein report a case of diffuse cutaneous systemic sclerosis under hemodialysis due to a previous history of scleroderma renal crisis, whose interstitial lung disease was effectively and safely treated with a half dose of i.v. cyclophosphamide pulse. The dose of cyclophosphamide and the timing of hemodialysis leading to efficacy and low toxicity are discussed.
Assuntos
Antirreumáticos/administração & dosagem , Ciclofosfamida/administração & dosagem , Falência Renal Crônica/complicações , Doenças Pulmonares Intersticiais/tratamento farmacológico , Escleroderma Sistêmico/complicações , Feminino , Humanos , Falência Renal Crônica/terapia , Doenças Pulmonares Intersticiais/etiologia , Pessoa de Meia-Idade , Diálise RenalRESUMO
UNLABELLED: Propranolol hydrochloride is a nonselective ß-blocker that is used for the treatment of hypertension, arrhythmia, and angina pectoris. In Japan, it was recently approved for the treatment of childhood arrhythmia. It has been observed to produce drastic involution of infantile hemangiomas. The aim of this prospective study was to examine propranolol's superiority to classical therapy with pulsed dye laser and/or cryosurgery in treating proliferating infantile hemangiomas. Fifteen patients between the ages of 1 and 4 months with proliferating infantile hemangiomas received grinded propranolol tablets 2 mg/kg per day divided in three doses. Twelve patients with proliferating infantile hemangiomas receiving pulsed dye laser and/or cryosurgery were enrolled as controls. Baseline electrocardiogram, echocardiogram, and chest x-ray were performed. Monitoring of heart rate, blood pressure, and blood glucose was performed every 2 weeks. Efficacy was assessed by performing blinded volume measurements and taking photographs at every visit. Propranolol induced significantly earlier involution and redness reduction of infantile hemangiomas, compared to pulsed dye laser and cryosurgery. Adverse effects such as hypoglycemia, hypotension, or bradycardia did not occur. CONCLUSION: The dramatic response of infantile hemangiomas to propranolol and few side effects suggest that early treatment of infantile hemangiomas could result in decreased disfigurement. Propranolol should be considered as a first-line treatment of infantile hemangiomas.
Assuntos
Antagonistas Adrenérgicos beta/uso terapêutico , Criocirurgia , Hemangioma/tratamento farmacológico , Lasers de Corante/uso terapêutico , Propranolol/uso terapêutico , Neoplasias Cutâneas/tratamento farmacológico , Administração Oral , Esquema de Medicação , Feminino , Hemangioma/cirurgia , Humanos , Lactente , Masculino , Estudos Prospectivos , Neoplasias Cutâneas/cirurgia , Resultado do TratamentoAssuntos
Pustulose Exantematosa Aguda Generalizada/etiologia , Pustulose Exantematosa Aguda Generalizada/patologia , Anti-Inflamatórios/efeitos adversos , Anticorpos Monoclonais Humanizados/efeitos adversos , Adalimumab , Artrite Reumatoide/tratamento farmacológico , Feminino , Humanos , Pessoa de Meia-IdadeRESUMO
Mongolian spots are congenital and confluent hyperpigmented areas that are usually grayish blue in color. They are found most frequently in the sacral region in infants and typically disappear during childhood. Occasionally, they persist to adulthood. We retrospectively examined outcomes of laser treatment of persistent Mongolian spots. We used Q-switched alexandrite laser to treat persistent Mongolian spots of 16 Japanese patients at 14 years old or older. A good therapeutic outcome was achieved overall; however, postinflammatory hyperpigmentation and hypopigmentation were observed in two patients, respectively. While laser treatment was effective for all seven patients with extrasacral Mongolian spots, four out of ten patients with sacral Mongolian spots were refractory to treatment. Of these patients, two received laser irradiation only twice and abandoned treatment, simply because of unsatisfactory results without any adverse events. The other two patients received treatments at intervals of 3 months, which resulted in postinflammatory hyperpigmentation. Contrary to children, who generally show good response after two or three sessions of irradiation, we should consider more frequent irradiation, longer intervals between treatment sessions, and use of bleaching creams in the treatment of persistent sacral Mongolian spots in adults.
Assuntos
Lasers de Estado Sólido/uso terapêutico , Mancha Mongólica/radioterapia , Neoplasias Cutâneas/radioterapia , Adolescente , Adulto , Humanos , Hiperpigmentação/etiologia , Hipopigmentação/etiologia , Lasers de Estado Sólido/efeitos adversos , Pessoa de Meia-Idade , Estudos Retrospectivos , Região Sacrococcígea , Resultado do Tratamento , Adulto JovemRESUMO
Dermatofibroma, also called cutaneous fibrous histiocytoma or sclerosing hemangioma, is a fairly common, benign cutaneous tumor. Polypoid dermatofibroma is an unusual and poorly recognized form of this entity. We describe a peculiar case of this variant presenting with a unique morphology characterized by a round flat shape with a slim pedicle, although its histopathological picture was rather typical of that of ordinary dermatofibroma.
Assuntos
Histiocitoma Fibroso Benigno/patologia , Neoplasias Cutâneas/patologia , Idoso , Feminino , Histiocitoma Fibroso Benigno/cirurgia , Humanos , Neoplasias Cutâneas/cirurgiaAssuntos
Vesícula/sangue , Vesícula/patologia , Psoríase/sangue , Psoríase/patologia , Fator A de Crescimento do Endotélio Vascular/sangue , Vesícula/tratamento farmacológico , Vesícula/radioterapia , Colecalciferol/uso terapêutico , Ciclosporina/uso terapêutico , Fármacos Dermatológicos/uso terapêutico , Quimioterapia Combinada , Etretinato/uso terapêutico , Feminino , Humanos , Pessoa de Meia-Idade , Psoríase/tratamento farmacológico , Psoríase/radioterapia , Resultado do Tratamento , Terapia UltravioletaRESUMO
We herein report a 32-year-old Caucasian woman with human leukocyte antigen (HLA)-B51 who developed Behçet's disease after immigration to Japan. She was born and raised in Finland and had never experienced any clinical symptoms related to Behçet's disease before immigration to Japan. None of her family members in Finland had similar problems. Two years after the immigration, she developed typical clinical symptoms of Behçet's disease. This report strongly supports the canonical notion that the interaction of genetic factors, such as HLA-B51, with environmental factors specific to the countries in the Silk Road area, including Japan, may play a pivotal role in the pathogenesis of this disorder.
Assuntos
Síndrome de Behçet/diagnóstico , Síndrome de Behçet/imunologia , Antígeno HLA-B51/genética , Adulto , Síndrome de Behçet/etiologia , Emigração e Imigração , Feminino , Finlândia/etnologia , Humanos , Japão , População BrancaAssuntos
Úlcera da Perna/tratamento farmacológico , Úlcera da Perna/etiologia , Sulfonamidas/uso terapêutico , Síndrome de Werner/complicações , Cicatrização/fisiologia , Bosentana , Terapia Combinada , Desbridamento/métodos , Relação Dose-Resposta a Droga , Esquema de Medicação , Antagonistas dos Receptores de Endotelina , Seguimentos , Humanos , Úlcera da Perna/terapia , Masculino , Pessoa de Meia-Idade , Curativos Oclusivos , Receptores de Endotelina/uso terapêutico , Índice de Gravidade de Doença , Resultado do Tratamento , Síndrome de Werner/diagnósticoAssuntos
Sarcoidose/complicações , Sarcoidose/diagnóstico , Escleroderma Sistêmico/complicações , Escleroderma Sistêmico/diagnóstico , Anti-Inflamatórios/uso terapêutico , Feminino , Dermatoses da Mão/etiologia , Humanos , Doenças Pulmonares Intersticiais/etiologia , Pessoa de Meia-Idade , Debilidade Muscular/etiologia , Prednisona/uso terapêutico , Sarcoidose/tratamento farmacológico , Escleroderma Sistêmico/tratamento farmacológicoAssuntos
Calcinose/patologia , Escleroderma Sistêmico/patologia , Antibacterianos/uso terapêutico , Calcinose/diagnóstico , Calcinose/tratamento farmacológico , Calcinose/etiologia , Feminino , Humanos , Pessoa de Meia-Idade , Esclerodermia Difusa/complicações , Esclerodermia Difusa/patologia , Escleroderma Sistêmico/complicações , Tomografia Computadorizada por Raios XAssuntos
Autoanticorpos/imunologia , Dermatomiosite/imunologia , Isoleucina-tRNA Ligase/imunologia , Autoanticorpos/sangue , Ciclosporina/uso terapêutico , Dermatomiosite/sangue , Dermatomiosite/diagnóstico , Dermatomiosite/tratamento farmacológico , Quimioterapia Combinada , Feminino , Humanos , Imunossupressores/uso terapêutico , Doenças Pulmonares Intersticiais/diagnóstico , Doenças Pulmonares Intersticiais/tratamento farmacológico , Doenças Pulmonares Intersticiais/imunologia , Pessoa de Meia-Idade , Prednisona/uso terapêutico , Resultado do TratamentoRESUMO
The symbiosis between Armillaria species and an achlorophylous orchid Gastrodia elata BLUME has been reported. The main species described as a symbiont is Armillaria mellea (VAHL: FR.) KUMMER, known widely as a primary root rot pathogen. Samples collected from the rhizomorphs attached to the tuber of G. elata were separated and analyzed. Molecular analysis based on sequencing of the intergenic spacer 1 (IGS-1) and internal transcribed spacer (ITS) regions of the ribosomal DNA (rDNA) was performed, coupled with restriction fragment length polymorphism (RFLP) of the IGS-1 region. Cultural morphology and features of basidiomata were also used to characterize the isolates. Phylogenetic analysis and morphological data strongly suggested that the fungus present in the tubers of G. elata is Armillaria nabsnona. This is the first report of occurrence of this Armillaria species in association with G. elata.
Assuntos
Basidiomycota/química , Basidiomycota/genética , Gastrodia/química , Gastrodia/genética , DNA Intergênico/genética , DNA de Plantas/genética , Endonucleases/química , Endonucleases/genética , Dados de Sequência Molecular , Filogenia , Raízes de Plantas/química , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Esporos Fúngicos/genéticaRESUMO
De-O-sulfonated analogs (10a, Y(-)=CH(3)OSO(3) and 10b, Y(-)=Cl) of salacinol, a naturally occurring glycosidase inhibitor, and its diastereomer (12a, Y(-)=CH(3)OSO(3)) with L-thiosugar moiety (1,4-dideoxy-1,4-epithio-L-arabinitol) were prepared. Their inhibitory activities against intestinal maltase and sucrase were examined and compared with those of the parent alpha-glycosidase inhibitor, salacinol (1a). Compounds 10a and 10b showed a potent inhibitory activity equal to that of 1a against both enzymes, although 12a was a weak inhibitor against sucrase and maltase. These results indicated that the O-sulfonate anion moiety of 1a is not essential for the inhibitory activity.
Assuntos
Inibidores Enzimáticos/química , Inibidores Enzimáticos/farmacologia , Inibidores de Glicosídeo Hidrolases , Álcoois Açúcares/química , Sulfatos/química , Animais , Ânions/química , Inibidores Enzimáticos/síntese química , Ratos , Relação Estrutura-AtividadeRESUMO
The gene of an intracellular D(-)-3-hydroxybutyrate oligomer hydrolase (i3HBOH) was cloned and sequenced from a poly(3-hydroxybutyrate) (PHB)-degrading bacterium, Acidovorax sp. strain SA1. The i3HBOH gene has 876 nucleotides corresponding to the deduced sequence of 292 amino acids. In this amino acid sequence, the general lipase box sequence (G-X(1)-S-X(2)-G) was found, whose serine residue was determined to the active sites serine by site-directed mutagenesis. An i3HBOH was purified to electrophoretical homogeneity from SA1. The molecular mass of the purified enzyme was estimated to be 32 kDa by SDS-PAGE. The N-terminal amino acid sequence of the purified enzyme corresponded to the deduced N-terminal amino acid sequence in the cloned i3HBOH gene. This is the first cloning and sequencing of an intracellular D(-)-3-hydroxybutyrate oligomer hydrolase gene to date.