RESUMO
Human promyelocytic HL60 cells can be differentiated into macrophagelike cells by treatment with 12Otetra decanoylphorbol13acetate (TPA). Certain 5' upstream regions of the zinc finger protein (ZNF)encoding genes contain duplicated GGAA motifs, which are frequently found in the TPAresponding gene promoter regions. To examine transcriptional responses to TPA, 5'flanking regions of human zinc finger CCCHtype containing, antiviral, ZNF252, ZNF343, ZNF555, ZNF782 and zinc finger nfx1type containing 1 (ZNFX1) genes were isolated by polymerase chain reaction (PCR) and ligated into a multiplecloning site of the pGL4.10[luc2] vector. Transient transfection and a luciferase assay revealed that the ZNFX1 promoter most prominently responded to the TPA treatment. Deletion and point mutation experiments indicated that the duplicated GGAA motif in the 100bp region positively responded to TPA. In addition, reverse transcriptionquantitative PCR and western blotting showed that the mRNA and protein of ZNFX1 accumulate during the differentiation of HL60 cells. These results indicated that expression of the TPAinducible ZNFX1 gene, which belongs to the group of interferonresponsive genes, is regulated by the cisaction of the duplicated GGAA motif.
Assuntos
Antígenos de Neoplasias/genética , Antígenos de Neoplasias/metabolismo , Regiões Promotoras Genéticas/efeitos dos fármacos , Acetato de Tetradecanoilforbol/farmacologia , Diferenciação Celular , Repetições de Dinucleotídeos/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Células HL-60 , Humanos , Mutação Puntual , Deleção de Sequência , Ativação TranscricionalRESUMO
We present two cases of pathologic fractures extended to the metacarpal head related with enchondromas at the metacarpal neck treated by surgery. The timing of surgery varied between the two cases. The first was operated without delay, using tumor curettage and ß-TCP (tricalcium phosphate) packing in the cavity, followed by internal fixation of the fracture using a screw and Kirshner wires. In the second case, tumor curettage and ß-TCP packing was performed after fracture union. Favorable clinical outcomes were obtained for both cases.
Assuntos
Condroma/complicações , Fixação Interna de Fraturas , Fraturas Espontâneas/etiologia , Ossos Metacarpais/lesões , Adulto , Parafusos Ósseos , Fios Ortopédicos , Condroma/diagnóstico , Condroma/cirurgia , Feminino , Fraturas Espontâneas/diagnóstico , Fraturas Espontâneas/cirurgia , Humanos , Masculino , Ossos Metacarpais/cirurgiaRESUMO
Synovial osteochondromatosis (SOC) is a benign tumor characterized by synovial connective tissue metaplasia. SOC commonly affects major joints including the knee followed by the hip, elbow, and wrist. SOC cases in the hand are not reported as often as SOC of major joints. Particularly SOC of the carpometacarpal joint of the thumb is rare. We report on a 57-year-old female with primary SOC of the carpometacarpal joint of her left thumb. Surgical excision was performed and the patient had no symptoms with full range of motion of her left thumb. At 3 years of follow-up, there was no recurrence.