1.
Chem Commun (Camb)
; 47(16): 4742-4, 2011 Apr 28.
Artigo
em Inglês
| MEDLINE
| ID: mdl-21409251
RESUMO
A Staphylococcus aureus transpeptidase, sortase A (SrtA), catalyzes selective peptide/protein ligations that have been applied to cell imaging and protein engineering, while the ligations do not proceed to completion due to their reversibility. We successfully enhanced SrtA-mediated protein ligation through the formation of a ß-hairpin around the ligation site.
Assuntos
Aminoaciltransferases/metabolismo , Proteínas de Bactérias/metabolismo , Cisteína Endopeptidases/metabolismo , Proteínas/síntese química , Aminoaciltransferases/química , Proteínas de Bactérias/química , Sítios de Ligação , Biocatálise , Cisteína Endopeptidases/química , Modelos Moleculares , Engenharia de Proteínas , Estrutura Secundária de Proteína , Proteínas/química , Staphylococcus aureus/enzimologia , Temperatura
2.
Synthesis of a Trisaccharide Library by Using a Phenylsulfonate Traceless Linker on Synphase Crowns.
Angew Chem Int Ed Engl
; 40(17): 3230-3233, 2001 Sep 03.
Artigo
em Inglês
| MEDLINE
| ID: mdl-29712045
RESUMO
44 members are in the trisaccharide library synthesized by the method described here. Six differently protected glucoside building blocks were sulfonylated with 1, coupled to the solid support 2 (a crown compound) by Pd0 -catalyzed carbonylative amidation, glycosylated twice, and cleaved from the sulfonate linker with four nucleophiles.
3.
s.l; s.n; 1975. 4 p. tab.
Não convencional
em Inglês
| Sec. Est. Saúde SP, HANSEN, Hanseníase, SESSP-ILSLACERVO, Sec. Est. Saúde SP
| ID: biblio-1232598
