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1.
J Neural Transm (Vienna) ; 120(3): 361-74, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-22955958

RESUMO

We have previously reported free radical production after traumatic brain injury (TBI), which induces neural stem cell (NSC) degeneration and death. However, the effects of aging on NSC proliferation around the damaged area following TBI have not been investigated. Therefore, in this study, we used 10-week (young group) and 24-month-old (aged group) rat TBI models to investigate the effects of aging on NSC proliferation around damaged tissue using immunohistochemical and ex vivo techniques. Young and aged rats received TBI. At 1, 3 and 7 days after TBI, immunohistochemical and lipid peroxidation studies were performed. Immunohistochemistry revealed that the number of nestin-positive cells around the damaged area after TBI in the aged group decreased significantly when compared with those in the young group (P < 0.01). However, the number of 8-hydroxy-2'-deoxyguanosine-, 4-hydroxy-2-nonenal- and single-stranded DNA (ssDNA)-positive cells and the level of peroxidation around the damaged area after TBI significantly increased in the aged group, compared with those in the young group (P < 0.01). Furthermore, almost all ssDNA-positive cells in young and aged groups co-localized with NeuN and nestin staining. Ex vivo studies revealed that neurospheres, which differentiated into neurons and glia in culture, could only be isolated from injured brain tissue in young and aged groups at 3 days after TBI. These results indicate that, although there were fewer NSCs that have the potential to differentiate into neurons and glia, these NSCs escaped free radical-induced degeneration around the damaged area after TBI in the aged rat brain.


Assuntos
Lesões Encefálicas/metabolismo , Lesões Encefálicas/patologia , Peroxidação de Lipídeos/fisiologia , Células-Tronco Neurais/citologia , 8-Hidroxi-2'-Desoxiguanosina , Envelhecimento , Aldeídos/análise , Animais , Diferenciação Celular/fisiologia , DNA de Cadeia Simples/análise , Desoxiguanosina/análogos & derivados , Desoxiguanosina/análise , Desoxiguanosina/biossíntese , Modelos Animais de Doenças , Imunofluorescência , Imuno-Histoquímica , Proteínas de Filamentos Intermediários/análise , Proteínas de Filamentos Intermediários/biossíntese , Masculino , Proteínas do Tecido Nervoso/análise , Proteínas do Tecido Nervoso/biossíntese , Nestina , Células-Tronco Neurais/metabolismo , Ratos , Ratos Wistar
2.
Exp Anim ; 52(5): 429-32, 2003 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-14625411

RESUMO

Age-related alterations and differences of weights and those of amino acid concentrations in the cerebrospinal fluid (CSF) were evaluated between Sprague Dawley (SD) rats and Wistar Kyoto (WKY) rats from eight to twenty weeks of age. The weights of SD rats were heavier than WKY rats at all ages. The age-related alterations of the CSF concentration of many amino acids within each strain were significant but showed no significant trend with age. Between the strains, the concentration differences of excitatory and inhibitory amino acids were not frequent although the concentrations of arginine, alanine and threonine were significantly higher in SD rats than in WKY rats. These results suggest that the different CSF concentrations of amino acids may relate to characteristics of rat strains.


Assuntos
Envelhecimento/líquido cefalorraquidiano , Envelhecimento/fisiologia , Aminoácidos/líquido cefalorraquidiano , Ratos Endogâmicos WKY/líquido cefalorraquidiano , Ratos Sprague-Dawley/líquido cefalorraquidiano , Animais , Arginina/líquido cefalorraquidiano , Peso Corporal , Masculino , Ratos , Treonina/líquido cefalorraquidiano
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