RESUMO
A method based on the susceptibility of photosynthetic organisms to nitrofurantoin under illumination was established to screen mutants of Synechocystis sp. PCC 6803 deficient in the function of photosystem II, which were created by random PCR mutagenesis targeted to the psbAII gene coding for the D1 protein of the photosystem II reaction center. In this method, cyanobacterial colonies on a nitrocellulose membrane on a BG11 agar plate were treated with nitrofurantoin at 1.0 mM under white light at 40 microE x m(-2 ) x s(-1) for 2 h, and then kept under normal conditions without nitrofurantoin so that surviving cells could grow. This method was also shown to be useful for screening mutants deficient in the function of photosystem I.
Assuntos
Cianobactérias/genética , Nitrofurantoína/farmacologia , Fotossíntese/genética , Cianobactérias/efeitos dos fármacos , Cianobactérias/metabolismo , Farmacorresistência Bacteriana/genética , Cinética , Luz , Mutagênese , Complexo de Proteínas do Centro de Reação Fotossintética/genética , Complexo de Proteína do Fotossistema I , Complexo de Proteína do Fotossistema II , Fatores de TempoRESUMO
GFR (Gelatin-Resorcin-Formalin) glue consists of mixture of gelatin and resorcin. The mixture is hardened by the addition of medical form-aldehyde, creating tridimensional network. From August 1991 to February 1993, six patients of type A acute aortic dissection underwent repair of false lumen with GRF glue. Including one female, the mean age was 57.3 years old ranged 51 years old to 64 years old. The entry of aortic intima were found in the ascending aorta in 4 cases, in the greater curvature of aortic arch in one case, and in the lesser curvature of aortic arch in one case. We used GRF glue for adhesion of intimal and medial wall of dissecting aneurysm in all cases. In case 1, the ascending aorta was replaced with a ringed intraluminal graft, and in case 2, the ascending aorta was replaced with a graft. In case 3 and 4, the aortic wall was closed primarily using GRF glue after patch closure of an entry. In case 5 and 6, the ascending aorta including an aortic arch was replaced with a graft. We revealed that GRF glue could harden the thin wall of aorta in the acute phase.
