Assuntos
Fármacos Anti-HIV/efeitos adversos , Toxidermias/etiologia , Infecções por HIV/tratamento farmacológico , Oxazinas/efeitos adversos , Transtornos de Fotossensibilidade/induzido quimicamente , Alcinos , Fármacos Anti-HIV/uso terapêutico , Benzoxazinas , Ciclopropanos , Dermatite Fotoalérgica/etiologia , Dermatite Fotoalérgica/fisiopatologia , Toxidermias/fisiopatologia , Seguimentos , Infecções por HIV/diagnóstico , Humanos , Japão , Masculino , Pessoa de Meia-Idade , Oxazinas/uso terapêutico , Transtornos de Fotossensibilidade/fisiopatologia , Medição de Risco , Índice de Gravidade de DoençaRESUMO
We have recently developed a micropore ultraviolet irradiation technique. An isopore membrane filter with 3 microm diameter pores shields ultraviolet C radiation from cultured human fibroblasts, leading to partial irradiation within the cells with an average of about three exposed areas per nucleus. This study addressed the question of whether the spatial distribution of DNA damage within a cell nucleus is important in triggering ultraviolet-induced cytotoxicity. We have examined whether there are differences in cytotoxicity between partially ultraviolet-irradiated cells and uniformly irradiated cells after equal amounts of DNA damage were induced in the cell nuclei. We first determined DNA damage formation in normal human fibroblasts using an enzyme-linked immunosorbent assay. We found that 5 J per m2 ultraviolet irradiation produced an equivalent amount of cyclobutane pyrimidine dimers and (6-4) photoproducts per cell as 100 J per m2 with the membrane filter shield. At those doses, we found that both types of ultraviolet irradiation induced similar levels of cytotoxicity as assessed by a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay. Both types of ultraviolet-irradiated cells also had similar cell-cycle distribution and apoptosis as measured by flow cytometry. Moreover, no significant differences in repair kinetics for either type of photolesion were observed between the two different ultraviolet treatments. Similar results were obtained in Cockayne syndrome cells that are defective in transcription-coupled nucleotide excision repair. Present results indicate that in the range of photoproducts studied, the spatial distribution of DNA damage within a cell is less important than the amount of damage in triggering ultraviolet-induced cytotoxicity.
Assuntos
Dano ao DNA , Fibroblastos/efeitos da radiação , Apoptose/efeitos da radiação , Ciclo Celular/efeitos da radiação , Núcleo Celular , Células Cultivadas , Reparo do DNA , Relação Dose-Resposta à Radiação , Fibroblastos/citologia , Humanos , Raios UltravioletaRESUMO
A case of angiosarcoma arising from the nose of a 69-year-old man is presented in this report. The patient was treated with a combination of recombinant interleukin-2 (rIL-2, Celeuk), electron beam irradiation, and surgery. He died 27 months after diagnosis, but there was no apparent remote metastasis.
