A novel method for cliff vegetation estimation based on the unmanned aerial vehicle 3D modeling.

The cliff ecosystem is one of the least human-disturbed ecosystems in nature, and its inaccessible and often extreme habitats are home to many ancient and unique plant species. Because of the harshness of cliff habitats, their high elevation, steepness of slopes, and inaccessibility to humans, surveying cliffs is incredibly challenging. Comprehensive and systematic information on cliff vegetation cover is not unavailable but obtaining such information on these cliffs is fundamentally important and of high priority for environmentalists. Traditional coverage survey methods-such as large-area normalized difference vegetation index (NDVI) statistics and small-area quadratic sampling surveys-are not suitable for cliffs that are close to vertical. This paper presents a semi-automatic systematic investigation and a three-dimensional reconstruction of karst cliffs for vegetation cover evaluation. High-resolution imagery with structure from motion (SFM) was captured by a smart unmanned aerial vehicle (UAV). Using approximately 13,000 records retrieved from high-resolution images of 16 cliffs in the karst region Guilin, China, 16 models of cliffs were reconstructed. The results show that this optimized UAV photogrammetry method greatly improves modeling efficiency and the vegetation cover from the bottom to the top of cliffs is high-low-high, and very few cliffs have high-low cover at the top. This study highlights the unique vegetation cover of karst cliffs, which warrants further research on the use of SFM to retrieve cliff vegetation cover at large and global scales.

Survival prediction optimization of acute myeloid leukaemia based on T-cell function-related genes and plasma proteins.

Interactions between acute myeloid leukaemia (AML) cells and immune cells are postulated to corelate with outcomes of AML patients. However, data on T-cell function-related signature are not included in current AML survival prognosis models. We examined data of RNA matrices from 1611 persons with AML extracted from public databases arrayed in a training and three validation cohorts. We developed an eight-gene T-cell function-related signature using the random survival forest variable hunting algorithm. Accuracy of gene identification was tested in a real-world cohort by quantifying cognate plasma protein concentrations. The model had robust prognostic accuracy in the training and validation cohorts with five-year areas under receiver-operator characteristic curve (AUROC) of 0.67-0.76. The signature was divided into high- and low-risk scores using an optimum cut-off value. Five-year survival in the high-risk groups was 6%-23% compared with 42%-58% in the low-risk groups in all the cohorts (all p values <0.001). In multivariable analyses, a high-risk score independently predicted briefer survival with hazard ratios of death in the range 1.28-2.59. Gene set enrichment analyses indicated significant enrichment for genes involved in immune suppression pathways in the high-risk groups. Accuracy of the gene signature was validated in a real-world cohort with 88 pretherapy plasma samples. In scRNA-seq analyses most genes in the signature were transcribed in leukaemia cells. Combining the gene expression signature with the 2017 European LeukemiaNet classification significantly increased survival prediction accuracy with a five-year AUROC of 0.82 compared with 0.76 (p < 0.001). Our T-cell function-related risk score complements current AML prognosis models.

Automated extraction of Camellia oleifera crown using unmanned aerial vehicle visible images and the ResU-Net deep learning model.

As one of the four most important woody oil-tree in the world, Camellia oleifera has significant economic value. Rapid and accurate acquisition of C. oleifera tree-crown information is essential for enhancing the effectiveness of C. oleifera tree management and accurately predicting fruit yield. This study is the first of its kind to explore training the ResU-Net model with UAV (unmanned aerial vehicle) images containing elevation information for automatically detecting tree crowns and estimating crown width (CW) and crown projection area (CPA) to rapidly extract tree-crown information. A Phantom 4 RTK UAV was utilized to acquire high-resolution images of the research site. Using UAV imagery, the tree crown was manually delineated. ResU-Net model's training dataset was compiled using six distinct band combinations of UAV imagery containing elevation information [RGB (red, green, and blue), RGB-CHM (canopy height model), RGB-DSM (digital surface model), EXG (excess green index), EXG-CHM, and EXG-DSM]. As a test set, images with UAV-based CW and CPA reference values were used to assess model performance. With the RGB-CHM combination, ResU-Net achieved superior performance. Individual tree-crown detection was remarkably accurate (Precision = 88.73%, Recall = 80.43%, and F1score = 84.68%). The estimated CW (R 2 = 0.9271, RMSE = 0.1282 m, rRMSE = 6.47%) and CPA (R 2 = 0.9498, RMSE = 0.2675 m2, rRMSE = 9.39%) values were highly correlated with the UAV-based reference values. The results demonstrate that the input image containing a CHM achieves more accurate crown delineation than an image containing a DSM. The accuracy and efficacy of ResU-Net in extracting C. oleifera tree-crown information have great potential for application in non-wood forests precision management.

Estimating the Growing Stem Volume of the Planted Forest Using the General Linear Model and Time Series Quad-Polarimetric SAR Images.

Increasing the area of planted forests is rather important for compensation the loss of natural forests and slowing down the global warming. Forest growing stem volume (GSV) is a key indicator for monitoring and evaluating the quality of planted forest. To improve the accuracy of planted forest GSV located in south China, four L-band ALOS PALSAR-2 quad-polarimetric synthetic aperture radar (SAR) images were acquired from June to September with short intervals. Polarimetric characteristics (un-fused and fused) derived by the Yamaguchi decomposition from time series SAR images with different intervals were considered as independent variables for the GSV estimation. Then, the general linear model (GLM) obeyed the exponential distribution were proposed to retrieve the stand-level GSV in plantation. The results show that the un-fused power of double bounce scatters and four fused variables derived from single SAR image is highly sensitive to the GSV, and these polarimeric characteristics derived from the time series images more significantly contribute to improved estimation of GSV. Moreover, compared with the estimated GSV using the semi-exponential model, the employed GLM model with less limitations and simple algorithm has a higher saturation level (nearly to 300 m3/ha) and higher sensitivity to high forest GSV values than the semi-exponential model. Furthermore, by reducing the external disturbance with the help of time average, the accuracy of estimated GSV is improved using fused polarimeric characteristics, and the estimation accuracy of forest GSV was improved as the images increase. Using the fused polarimetric characteristics (Dbl×Vol/Odd) and the GLM, the minimum RRMSE was reduced from 33.87% from single SAR image to 24.42% from the time series SAR images. It is implied that the GLM is more suitable for polarimetric characteristics derived from the time series SAR images and has more potential to improve the planted forest GSV.

[Estimation of Hunan forest carbon density based on spectral mixture analysis of MODIS data].

With the fast development of remote sensing technology, combining forest inventory sample plot data and remotely sensed images has become a widely used method to map forest carbon density. However, the existence of mixed pixels often impedes the improvement of forest carbon density mapping, especially when low spatial resolution images such as MODIS are used. In this study, MODIS images and national forest inventory sample plot data were used to conduct the study of estimation for forest carbon density. Linear spectral mixture analysis with and without constraint, and nonlinear spectral mixture analysis were compared to derive the fractions of different land use and land cover (LULC) types. Then sequential Gaussian co-simulation algorithm with and without the fraction images from spectral mixture analyses were employed to estimate forest carbon density of Hunan Province. Results showed that 1) Linear spectral mixture analysis with constraint, leading to a mean RMSE of 0.002, more accurately estimated the fractions of LULC types than linear spectral and nonlinear spectral mixture analyses; 2) Integrating spectral mixture analysis model and sequential Gaussian co-simulation algorithm increased the estimation accuracy of forest carbon density to 81.5% from 74.1%, and decreased the RMSE to 5.18 from 7.26; and 3) The mean value of forest carbon density for the province was 30.06 t · hm(-2), ranging from 0.00 to 67.35 t · hm(-2). This implied that the spectral mixture analysis provided a great potential to increase the estimation accuracy of forest carbon density on regional and global level.