[Cerebrospinal fluid TP53 gene mutation in patients with lung cancer associated meningitis and its clinical implications].

Objective: To observe the role of cerebrospinal fluid (CSF) TP53 gene mutation in lung cancer associated meningitis. Methods: A retrospective analysis was performed on 35 patients diagnosed with lung cancer associated meningitis at the Second Hospital of Hebei Medical University from December 2015 to December 2018.All patients underwent the next-generation sequencing of CSF, and TP53 gene was found to be mutant or wild type, including 23 patients with TP53 mutant type and 12 patients with TP53 wild type. The clinical characteristics, CSF leukocyte, protein, glucose, chloride, Karnofsky performance (KPS) and overall survival were observed. Results: Headache, nausea and vomiting were the main clinical manifestations in both groups.There were no significant differences in CSF pressure, leukocyte, biochemical indicators and KPS between the two groups. The average time from diagnosis of lung cancer to diagnosis of lung cancer associated meningitis in the TP53 mutant group was significantly shorter than that in the TP53 wild type group (5.79 months vs 25.5 months).The median survival time of patients in the TP53 mutant group from lung cancer diagnosis to the observation endpoint was 19.77 months, while it was 88.73 months in the TP53 wild type group, and the difference was statistically significant (P=0.043). Conclusions: Mutation in the tumor suppressor gene TP53 can be detected in the CSF of patients with lung cancer associated meningitis. Patients with such mutation have earlier meningeal metastasis and shorter median survival time.

[Relationship between bile acid metabolism and chronic non - cholestatic liver disease].

Bile acid is a general term for a large class of cholic acid in bile and exerts its unique physiological functions by binding the relevant receptors and bile acid transporters. Bile acids not only promote the absorption of nutrients in the human body, but also as an important signaling molecule in the regulation of inflammatory processes and liver regeneration. Several studies have found that bile acid metabolism is involved in the occurrence and development of chronic non-cholestatic liver diseases. In this article, the latest research results are mentioned, and the relationship between nuclear receptors, membrane receptors, and bile acid transporters and chronic non-cholestatic liver disease that play a key role in the bile acid metabolism were emphatically reviewed.

Self reported musculoskeletal problems amongst typist and possible risk factors.

A telephone interview was conducted to explore the occupational musculoskeletal problems and potential risk factors among typists in a government department. Of the 330 typists, 170 (51.5%) were interviewed; all of them were female with about 84% in their twenties and thirties. Local fatigue and musculoskeletal pain were found to be quite prevalent among typists. More than 50% of the typists had local fatigue affecting the shoulders, neck, back and fingers (in order of decreasing frequency), and most of these occurred within 1-2 h of continuous typing. Among the musculoskeletal problems, low back pain was the most common (53%), followed by neck pain (50%), arm pain (27.6%) and finger pain (27.6%). Univariate analyses showed that poor matching of desk height with chair height was found to be related to low back pain, neck pain and arm pain; it was also related to local fatigue of the shoulder and neck regions. Fatigue and pain at the lower back were related to bending of the back at work. Finger pain and arm pain were related to the period of time which they had worked as a typist. Multivariate analyses were done with logistic regression to sort out the relative influence on the outcome of the different risk factors adjusted for age and for each other.