RESUMO
AIM: To evaluate the effect of a ketogenic diet (KD) in women with polycystic ovary syndrome (PCOS) and liver dysfunction who were obese. METHODS: Women with PCOS and liver dysfunction who were obese were enrolled in this prospective, open-label, parallel-group, controlled pilot trial, and randomly received KD (KD group) or conventional pharmacological treatment (Essentiale plus Yasmin, control group) in a 1:1 ratio for 12 weeks. The primary endpoint was the liver function markers. Secondary endpoints included the menstrual cycle, anthropometric characteristics, body composition, hormonal levels, and metabolic biomarkers. RESULTS: Of the 20 eligible participants enrolled, 18 participants completed the study. The KD group reported a significant reduction in anthropometric characteristics and body composition from baseline to week 12 (all p < 0.05). In addition, there were significant reductions in menstrual cycle, plasma estradiol, and progesterone levels in two groups (all p < 0.05), but no significant between-group difference was observed. KD significantly reduced the liver function markers compared with control group (p < 0.05). The signs of fatty liver disappeared in six out of seven fatty liver participants in KD group after 12 weeks of intervention, while only one of 10 fatty liver participants in control group disappeared. CONCLUSIONS: In addition to improving the menstrual cycle, KD had the additional benefits of reducing blood glucose and body weight, improving liver function, and treating fatty liver compared to traditional pharmacological treatment in women with PCOS and liver dysfunction who were obese.
Assuntos
Dieta Cetogênica , Hepatopatias , Síndrome do Ovário Policístico , Dieta Redutora , Feminino , Humanos , Obesidade/complicações , Projetos Piloto , Síndrome do Ovário Policístico/complicações , Estudos ProspectivosRESUMO
In this study, the complete mitochondrial DNA genome sequence of Xenophysogobio nudicorpa was first determined by DNA sequencing based on the PCR fragments. The complete mitochondrial DNA (mtDNA) genome sequence of X. nudicorpa is a circular molecule of 16,616 bp in length. It consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a control region (D-loop). The gene nucleotide composition of X. nudicorpa is 30.5% A, 26.0% C, 16.8% G, and 26.7% T, with a relatively high A+T content (57.2%). The results could provide useful data for studying genetic diversity and phylogenetics in X. nudicorpa and related species.
Assuntos
Cyprinidae/genética , Genoma Mitocondrial , Animais , Composição de Bases/genética , Pareamento de Bases/genética , Sequência de Bases , DNA Mitocondrial/genética , RNA de Transferência/genéticaRESUMO
The complete mitochondrial genome of Platysmacheilus nudiventris (Teleostei, Cyprinidae, Gobioninae), an endemic Chinese cyprinid fish, was first determined by DNA sequencing based on the PCR fragments. The total length of the mitochondrial genome is 16,603 bp, including 22 transfer RNA genes, 2 ribosomal RNA genes, 13 protein-coding genes and a non-coding control region (D-loop). The overall-based composition is 30.2% A, 26.9% T, 16.7% G and 26.1% C, with high A+T content (57.1%). The results will provide useful data for further studies on species identification, evolutionary and population genetic studies of P. nudiventris and its related species.
Assuntos
Cyprinidae/genética , Genoma Mitocondrial , Animais , Composição de Bases/genética , Sequência de Bases , DNA Mitocondrial/genética , Genes Mitocondriais , RNA de Transferência/genéticaRESUMO
In this study, the complete mitochondrial DNA genome sequence of Sinibrama taeniatus was first determined by DNA sequencing based on the PCR fragments. The complete mitochondrial DNA (mtDNA) genome sequence of S. taeniatus was a circular molecule of 16,623 bp in length. It consisted of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a control region (D-loop). The gene nucleotide composition of S. taeniatus was 31.3% A, 26.8% C, 16.1% G, and 25.8% T, with a relatively high A + T content (57.1%). The results could provide useful data for studying genetic diversity and phylogenetics in S. taeniatus and related species.
Assuntos
Cyprinidae/genética , Genoma Mitocondrial , Animais , Composição de Bases , Códon de Iniciação , Códon de Terminação , DNA Mitocondrial/química , DNA Mitocondrial/isolamento & purificação , DNA Mitocondrial/metabolismo , Variação Genética , Fases de Leitura Aberta/genética , RNA Ribossômico/química , RNA Ribossômico/isolamento & purificação , RNA Ribossômico/metabolismo , RNA de Transferência/química , RNA de Transferência/isolamento & purificação , RNA de Transferência/metabolismo , Análise de Sequência de DNARESUMO
In this study, the total mitochondrial genome sequence of Pseudobagrus emarginatus (Siluriformes: Bagridae) was firstly sequenced and determined. The complete mtDNA genome sequence of P. emarginatus is 16,534 bp in length. It consists of 22 transfer RNA genes, 2 ribosomal RNA genes, 13 protein-coding genes, and a non-coding control region (D-loop). The overall-based composition was 31.5% A, 26.8% T, 14.9% G and 26.8% C, with a high A+T content (58.3%), which was nearly the same as other reported catfishes. These results will provide useful data to the natural resources conservation and systematics analysis of P. emarginatus and its related species in future.
Assuntos
Peixes-Gato/genética , Genoma Mitocondrial/genética , Análise de Sequência de DNA , Animais , China , Conservação dos Recursos Naturais , Genes de RNAr/genética , Anotação de Sequência Molecular , Dados de Sequência Molecular , Fases de Leitura Aberta/genética , RNA de Transferência/genéticaRESUMO
In the present study, the complete mitochondrial genome sequence of Rhinogobio typus (Teleostei, Cyprinidae, Gobioninae) was obtained using a PCR-based method. The total length of the mitochondrial genome is 16,608 bp, including 22 transfer RNA genes, 2 ribosomal RNA genes, 13 protein-coding genes, and a non-coding control region (D-loop), which is similar to that observed in many other reported fishes. The overall-based composition is 31.3% A, 26.6% T, 15.9% G and 26.2% C, with high A + T content (57.9%). These results will provide a useful tool for species identification, evolutionary and population genetic studies of R. typus and its related species.
Assuntos
Cyprinidae/genética , Genoma Mitocondrial , Animais , Sequência de Bases , DNA Mitocondrial/genética , Fases de Leitura Aberta/genética , RNA Ribossômico/genética , RNA de Transferência/genéticaRESUMO
The complete mitochondrial genome of Pareuchiloglanis sinensis, an endemic Chinese sisorid fish, was determined by DNA sequencing based on the PCR fragments. The complete mtDNA genome sequence of P. sinensis is 16,593 bp in length, with a gene content of 13 protein-coding, 2 ribosomal RNA, and 22 transfer RNA genes, and a typical gene arrangement identical to many other reported fishes. The overall base composition of the heavy strand was 30.6% A, 24.2% T, 16.0% G and 29.2% C, with a relatively high A + T content (54.8%). This will provide a useful tool for understanding the genetic diversity, population structure and conservation status of P. sinensis in future.
Assuntos
Peixes-Gato/genética , Genoma Mitocondrial , Animais , Pareamento de Bases/genética , Sequência de Bases , DNA Circular/genética , DNA Mitocondrial/genética , Fases de Leitura Aberta/genética , RNA de Transferência/genéticaRESUMO
Glyptothorax sinense (Siluriformes, Sisoridae), is a kind of small-sized freshwater fish which mainly distributes in the middle and upper reaches of the Yangtze River in China. In this study, the complete mitochondrial genome of G. sinense was first determined using a PCR-based method. The complete mtDNA sequence is 16,531 bp in length, including 22 transfer RNA genes, 2 ribosomal RNA genes, 13 protein-coding genes, and a non-coding control region (D-loop). The overall-based composition was 31.61% A, 26.66% T, 15.38% G and 26.34% C, with a relatively high A + T content (58.27%). This will provide a useful tool for evolutionary and population genetic studies of G. sinense.
