A novel all-trans retinoid acid derivative induces apoptosis in MDA-MB-231 breast cancer cells.

AIMS: To explore the effect and probable mechanism of a synthetic retinoid 4-amino-2-tri-fluoromethyl- phenyl ester (ATPR) on apoptosis of MDA-MB-231 breast cancer cells. MATERIALS AND METHODS: MTT assays were performed to measure the proliferation of MDA-MB-231 cells treated with different concentrations of all- trans retinoic acid (ATRA) and ATPR. Morphologic changes were observed by microscopy. The apoptosis rates and cell cycling of MDA-MB-231 cells treated with ATRA or ATPR were assessed using flow cytometry analysis. Expression of retinoic acid receptor and phosphorylation of ERK, JNK, p38 proteins were detected by Western blotting. RESULTS: Treatment of the cells with the addition of 15 µmol/L ATPR for 48 h clearly demonstrated reduced cell numbers and deformed cells, whereas no changes in the number and morphology were observed after treatment with ATRA. The apoptosis rate was 33.2% after breast cancer MDA-MB-231 cells were treated by ATPR (15 µmol/L) whereas ATRA (15 µmol/L) had no apoptotic effect. ATPR inhibited the phosphorylation of ERK, JNK, and p38 while ATRA had no significant effect. ATPR inhibited the expression of BiP and increased the expression of Chop at the protein level compared with control groups, ATRA and ATPR both decreased the protein expression of RXR α, ATPR reduced the protein expression of RARß and RXRß while ATRA did not decrease RARß or RXRß. CONCLUSIONS: ATPR could induce apoptosis of breast cancer MDA-MB-231 cells, possible mechanisms being binding to RARß/RXRß heterodimers, then activation of ER stress involving the MAPK pathway.

Efficacy observation of nonspecific low back pain treated with the dragon-tiger fighting needling method / 中国针灸

<p><b>OBJECTIVE</b>To compare the difference in the clinical efficacy on nonspecific low back pain (NLBP) treated with the dragon-tiger fighting needling method, the uniform reinforcing-reducing method and the intermediate frequency physiotherapy.</p><p><b>METHODS</b>Ninety cases of NLBP were randomly divided into a dragon-tiger fighting needling group (group A), an uniform reinforcing-reducing needling group (group B) and an intermediate frequency physiotherapy group (group C), 30 cases in each one. In the group A, the dragon-tiger fighting needling method was used. In the group B, the uniform reinforcing-reducing method was applied. Two groups of acupoints were prescribed. One group included Shenshu (BL 23), Dachangshu (BL 25), Weizhong (BL 40) and Ashi points. The other group included Qihaishu (BL 24), Guanyuanshu (BL 26), Kunlun (BL 60), Yaoyangguan (GV 3). These two groups of acupoints were used alternatively in the above two groups. In the group C, the intermediate frequency physiotherapy was adopted in the pain area of the lumbar region. The treatment was given once per day in each group. Six treatments made one session. Totally, 2 sessions were required. The Visual Analogue Scale (VAS), the Oswestry Disability Index (ODD and the clinical efficacy were observed in each group.</p><p><b>RESULTS</b>The scores of VAS and ODI were reduced obviously after treatment in each group (P < 0.05, P < 0.01). The score reducing in the group A was much more remarkable than those in the other two groups (all P < 0.05). The clinical curative rate was 30.0% (9/30), 23.3% (7/30) and 16.7% (5/30) in the group A, the group B and the group C, respectively. In comparison, the clinical efficacy in the group A was superior to that in either of the other groups (all P< 0.05).</p><p><b>CONCLUSION</b>The dragon-tiger fighting needling method achieves the much better efficacy on NLBP compared with either the uniform reinforcing-reducing method or the intermediate frequency physiotherapy. It is one of the more effective needling method for analgesia.</p>