RESUMO
Controlled cortical impingement is a widely accepted method to induce traumatic brain injury to establish a traumatic brain injury animal model. A strike depth of 1 mm at a certain speed is recommended for a moderate brain injury and a depth of > 2 mm is used to induce severe brain injury. However, the different effects and underlying mechanisms of these two model types have not been proven. This study investigated the changes in cerebral blood flow, differences in the degree of cortical damage, and differences in motor function under different injury parameters of 1 and 2 mm at injury speeds of 3, 4, and 5 m/s. We also explored the functional changes and mitochondrial damage between the 1 and 2 mm groups in the acute (7 days) and chronic phases (30 days). The results showed that the cerebral blood flow in the injured area of the 1 mm group was significantly increased, and swelling and bulging of brain tissue, increased vascular permeability, and large-scale exudation occurred. In the 2 mm group, the main pathological changes were decreased cerebral blood flow, brain tissue loss, and cerebral vasospasm occlusion in the injured area. Substantial motor and cognitive impairments were found on day 7 after injury in the 2 mm group; at 30 days after injury, the motor function of the 2 mm group mice recovered significantly while cognitive impairment persisted. Transcriptome sequencing showed that compared with the 1 mm group, the 2 mm group expressed more ferroptosis-related genes. Morphological changes of mitochondria in the two groups on days 7 and 30 using transmission electron microscopy revealed that on day 7, the mitochondria in both groups shrank and the vacuoles became larger; on day 30, the mitochondria in the 1 mm group became larger, and the vacuoles in the 2 mm group remained enlarged. By analyzing the proportion of mitochondrial subgroups in different groups, we found that the model mice had different patterns of mitochondrial composition at different time periods, suggesting that the difference in the degree of damage among traumatic brain injury groups may reflect the mitochondrial changes. Taken together, differences in mitochondrial morphology and function between the 1 and 2 mm groups provide a new direction for the accurate classification of traumatic brain injury. Our results provide reliable data support and evaluation methods for promoting the establishment of standard mouse controlled cortical impingement model guidelines.
RESUMO
Based on the MODIS data and field measurement, the net primary production (NPP) in 2000 and 2006 in the middle reaches of Yarlung Zangbo River and its two tributaries in Tibet were estimated by using a mathematic model. The results showed that in study area, the NPP decreased gradually from valley to ridge, which was accorded with the gradients of precipitation and temperature. The annul NPP per unit area was averagely 86.8 g C m(-2) a(-1), and was 2.15 g C m(-2) a(-1) higher in 2006 than in 2000. Farmland ecosystem had the highest annual NPP per unit area (243.1 g C m(-2) a(-1)), while desert ecosystem had the lowest one (36.5 g C m(-2) a(-1)). The average total NPP in the two years was 512.8 x 10(10) g C a(-1), with the value in 2006 being 12.7 x 10(10) g C a(-1) higher than that in 2000. Meadow ecosystem had the highest annual total NPP (194.4 x 10(10) g C a(-1)), while desert ecosystem had the lowest one (30.3 x 10(10) g C a(-1)). In 2000-2006, the NPP value in the areas with strong human disturbance (0-4 km away from road) had a decreasing trend, while that in the areas difficult for human to reach was in adverse.
