RESUMO
OBJECTIVE: To develop a rapid test kit for antibody to HIV by nano immunomagnetic lateral flow method. METHODS: A rapid test kit was developed by conjugation of the HIV antigen gp41 and gp36 to 200nm super paramagnetic particles by carbodiimide (EDC) and coating of the HIV antigen gp41 and gp36 to nitrocellulose membrane. Then the kit was evaluated with serials of experiments. RESULTS: The kit was qualified with examination of national reference panel of anti-HIV antibody for colloidal gold diagnostic kit. The sensitivity was 100% by tested with 20 HIV antibody positive sera, the specificity was 98.5% by tested with 600 HIV antibody negative sera, respectively. The stability of the kit was over 12 month by storage at room temperature. CONCLUSION: A diagnostic kit for antibody to HIV was developed with the advantages of convenience, rapid test, good stability and point of care.
Assuntos
Anticorpos Anti-HIV , Infecções por HIV/diagnóstico , Soropositividade para HIV/sangue , HIV/isolamento & purificação , Separação Imunomagnética/métodos , Biologia Molecular/métodos , Nanotecnologia , Anticorpos Anti-Idiotípicos/imunologia , Coloide de Ouro/química , HIV/imunologia , Proteína gp41 do Envelope de HIV , HIV-1/imunologia , HIV-1/isolamento & purificação , Kit de Reagentes para DiagnósticoRESUMO
AIM: To study the effects of stress on the cognitive function and physical fitness and its biological mechanisms, provide scientific basis for seeking protective measures to reduce stress-induced damage. METHODS: The model of restraint stress was adopted in our experiment. Step-down test and exhaustive swimming test were used to measure the learning and memory function and physical fitness in mice, respectively. The contents of GC in plasma, hippocampus LTP, ECG, myocardial ultra-structure, cell apoptosis rate and the level of Hsp70 expression of myocardium in rats were detected. RESULTS: Compared with control group, the impairment of learning and memory function and decline of exercise tolerance were observed in restraint stress group. The elevation in plasma GC levels, ECG abnonrmality, and cell apoptosis rate were also observed under restraint stress. Furthermore, myocardial structure was damaged, and myocardial Hsp70 expression and hippocampus LTP were suppressed in restraint stress group than those in the control. CONCLUSION: Stress may cause the neuro-endocrine dysfunction and homeostasis disorder, and then, lead to the cognitive function and physical fitness damage consequently.
Assuntos
Cognição/fisiologia , Aptidão Física/fisiologia , Estresse Fisiológico/fisiologia , Animais , Proteínas de Choque Térmico HSP70/metabolismo , Hipocampo/fisiologia , Potenciação de Longa Duração/fisiologia , Masculino , Camundongos , Miocárdio/metabolismo , Distribuição Aleatória , Ratos , Ratos Wistar , Restrição FísicaRESUMO
To investigate the upregulated genes associated with cold acclimation, a cold acclimation model was established based on Balb/C mouse. mRNA of muscle and liver were isolated, and the upregulated genes of these tissues were studied by representational differential analysis (RDA). The upregulated genes then were sequenced and searched by Blast software in GenBank database. The results showed that some genes were upregulated and possibly associated with cold acclimation. Three of these genes, transferrin, fibrinogen B-beta-chains and a new gene fragment (Genbank ID: AF454762), were confirmed to be upregulated by RNA slot-blot analysis. The finding of these genes might contribute to further understanding of the molecular mechanisms of cold acclimation.
