RESUMO
To help determine whether the typical arthropod arrangement was a synapomorphy for the whole Tettigoniidae, we sequenced the mitochondrial genome (mitogenome) of the quiet-calling katydids, Xizicus fascipes (Orthoptera: Tettigoniidae: Meconematinae). The 16,166-bp nucleotide sequences of X. fascipes mitogenome contains the typical gene content, gene order, base composition, and codon usage found in arthropod mitogenomes. As a whole, the X. fascipes mitogenome contains a lower A+T content (70.2%) found in the complete orthopteran mitogenomes determined to date. All protein-coding genes started with a typical ATN codon. Ten of the 13 protein-coding genes have a complete termination codon, but the remaining three genes (COIII, ND5 and ND4) terminate with incomplete T. All tRNAs have the typical clover-leaf structure of mitogenome tRNA, except for tRNA(Ser(AGN)), in which lengthened anticodon stem (9 bp) with a bulged nuleotide in the middle, an unusual T-stem (6 bp in constrast to the normal 5 bp), a mini DHU arm (2 bp) and no connector nucleotides. In the A+T-rich region, two (TA)n conserved blocks that were previously described in Ensifera and two 150-bp tandem repeats plus a partial copy of the composed at 61 bp of the beginning were present. Phylogenetic analysis found: i) the monophyly of Conocephalinae was interrupted by Elimaea cheni from Phaneropterinae; and ii) Meconematinae was the most basal group among these five subfamilies.
Assuntos
Genoma Mitocondrial , Gryllidae/genética , Sequência de Aminoácidos , Animais , Composição de Bases , Sequência de Bases , Sequência Conservada , Primers do DNA/genética , DNA Mitocondrial/genética , Feminino , Proteínas de Insetos/genética , Proteínas Mitocondriais/genética , ATPases Mitocondriais Próton-Translocadoras/genética , Anotação de Sequência Molecular , Dados de Sequência Molecular , NADH Desidrogenase/genética , Conformação de Ácido Nucleico , Filogenia , RNA Ribossômico/genética , RNA de Transferência de Serina/genética , Análise de Sequência de DNARESUMO
The mitochondrial cytochrome oxidase I (mtDNA-COI) gene was used to investigate the intraspecific genetic subdivision among 12 geographical populations of Gampsocleis sedakovii distributed in Jilin, Liaoning, Inner Mongolia, Hebei, and Sichuan provinces, China. As for the 626 bp mtDNA-COI sequences of 36 individuals, 29 haplotypes and 71 (11.3%) polymorphic sites were detected, including 37 parsimony informative and 34 singleton variable sites. The results of the analysis of molecular variance (AMOVA) showed that the percentage of variation within populations (37.23%) was less than that among populations. The Fst value was 0.62770 (P< 0.001), and the genetic divergence among populations was significant. Maximum parsimony (MP) analysis revealed two clearly differentiated branch (â and â ¡) with high bootstrap support (100%). However, these two branches did not correspond in the subspecies distinction based on morphological character. Based on the habitats analysis, we preliminary inferred that morphological divergence between Gampsocleis se-dakovii sedakovii and Gampsocleis sedakovii obscura was the result of habitat dissimilarity. The majority of the G. se-dakovii individuals collected in NTL (Tongliao, Inner Mongolia) were identified as the members of branch â ¡, but one individual (NTL-3) belonged to branch â . Thus, at least this locality was occupied by two phylogenetically independent individuals. Haplotypes (H10) were shared by three localities, NTL, NEWK (Ewenke, Inner Mongolia) and JJL (Jilin, Jilin), indicated that they shared a common ancestor. We approved that Northeastern China (Manchuria China) was the center of G. sedakovii genetic differentiation, but disapproved the subspecies distinction based on morphological character.
