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1.
BMJ ; 386: q1384, 2024 07 03.
Artigo em Inglês | MEDLINE | ID: mdl-38960624
2.
Molecules ; 29(12)2024 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-38930905

RESUMO

BACKGROUND: Achyranthes bidentata (AR) is a traditional Chinese herb used for the treatment of hypertension and cerebral ischemia, but its pharmacological effects are not known. AIM OF STUDY: We aimed to detect and accurately identify the components and metabolites of AR in the plasma and brain tissue of Sprague Dawley rats. METHODS: We employed ultrahigh performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HR-MS) to detect AR components in the plasma and brain tissue of rats. The absorption and metabolites in the plasma and brain tissue of normal control rats and rats that underwent middle cerebral artery occlusion (MCAO) were characterized and compared. RESULTS: A total of 281 compounds, including alkaloids, flavonoids, terpenoids, phenylpropanes, sugars and glycosides, steroids, triterpenes, amino acids, and peptides, was identified in samples of Achyranthes bidentata (TCM-AR). Four types of absorbable prototype components and 48 kinds of metabolites were identified in rats in the normal control plasma group which were given AR (AR plasma group), and five kinds of metabolites were identified in rats of the normal control brain tissue group which were given AR (AR brain group). Three absorbed prototype components and 13 metabolites were identified in the plasma of rats which underwent MCAO and were given AR (MCAO + AR plasma group). Six absorbed prototype components and two metabolites were identified in the brain tissue of rats who underwent MCAO and were administered AR (MCAO + AR brain group). These results showed that, after the oral administration of AR, the number of identified components in plasma was more than that in brain tissue. The number of prototype components in the AR plasma group was higher than that in the MCAO + AR plasma group, which may indicate that metabolite absorption in rats undergoing MCAO was worse. The number of prototype components in the MCAO + AR brain group was higher than that in the AR brain group, indicating that the blood-brain barrier was destroyed after MCAO, resulting in more compounds entering brain tissue. CONCLUSIONS: UHPLC-HR-MS was used to rapidly analyze the components and metabolites of AR in the blood and brain of rats under normal and pathologic conditions, and to comprehensively characterize the components of TCM-AR. We also analyzed and compared the absorbable components and metabolites of normal rats under cerebral ischemia-reperfusion injury to explore the potential mechanism of action. This method could be applied to various Chinese herbs and disease models, which could promote TCM modernization.


Assuntos
Achyranthes , Encéfalo , Ratos Sprague-Dawley , Animais , Achyranthes/química , Cromatografia Líquida de Alta Pressão/métodos , Ratos , Encéfalo/metabolismo , Masculino , Espectrometria de Massas/métodos , Medicamentos de Ervas Chinesas/farmacocinética , Medicamentos de Ervas Chinesas/química , Infarto da Artéria Cerebral Média/metabolismo , Infarto da Artéria Cerebral Média/sangue , Flavonoides/sangue , Flavonoides/farmacocinética , Flavonoides/metabolismo , Alcaloides/sangue , Alcaloides/farmacocinética , Alcaloides/química , Alcaloides/metabolismo
3.
Anal Chem ; 96(27): 11083-11091, 2024 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-38924704

RESUMO

It is urgent to pursue appropriate gate photoactive materials for gate-to-channel signal modulation to achieve superior transconductance performances of organic photoelectrochemical transistor (OPECT) sensors. Notably, a hole transfer layer (HTL) participant CdZnS/sulfur-doped Ti3C2 MXene (S-MXene) gate was designed and developed in this work, which exhibited a remarkable signal modulation performance by up to 3 orders of magnitude. Because of the incorporation of S-MXene with an enhanced electrical conductivity as the effective HTL, the signal modulation capabilities of the CdZnS/S-MXene photoactive gate were superior to those of CdZnS and CdZnS/MXene. This incorporation inhibited the recombination of the interfacial charge and facilitated the transfer of photogenerated holes, thus enhancing the photoelectric conversion performance. This enhancement facilitated fast electron transfer with a larger effective photovoltage to augment the dedoping ability of channel ions. Based on these findings, an aptasensing platform that exhibited good performance was constructed using the proposed OPECT device, with ofloxacin as a model target and an aptamer for specific recognition. The developed OPECT aptasensor had various advantages, including a high sensitivity, good linear range (1.0 × 10-13 to 1.0 × 10-6 M), and low limit of detection (3.3 × 10-15 M). This study provided a proof-of-concept for the generalized development of HTL participant gates for OPECT sensors and other related applications.

4.
Zhongguo Gu Shang ; 37(2): 119-23, 2024 Feb 25.
Artigo em Chinês | MEDLINE | ID: mdl-38425060

RESUMO

OBJECTIVE: To investigate the preliminary clinical effect of closed reduction and cannulated nail internal fixation for femoral neck fracture assisted by robot navigation and positioning system. METHODS: From July 2019 to January 2020, 16 cases of femoral neck fracture (navigation group) were treated with closed reduction and internal fixation guided by robot system, including 7 males and 9 females, aged 25 to 72 years old with an average of (53.61±5.45) years old;Garden classification of fracture:3 cases of typeⅠ, 3 cases of typeⅡ, 8 cases of type Ⅲ, 2 cases of type Ⅳ. Non navigation group (control group):20 cases of femoral neck fracture were treated with closed reduction and hollow nail internal fixation, 8 males and 12 females, aged 46 to 70 years old with an average of (55.23±4.64) years old;Garden typeⅠin 2 cases, typeⅡin 4 cases, type Ⅲ in 11 cases, type Ⅳ in 3 cases. The operation time, fluoroscopy times, guide needle drilling times, screw adjustment times, intraoperative bleeding volume and other indicators of two groups were evaluated. RESULTS: Both groups were followed up for 12 to 18 months with an average of (15.6±2.8) months. The fractures of both groups were healed without delayed union and nonunion. There was no significant difference in healing time between two groups(P=0.782). There was no significant difference in Harris scores between two groups at the last follow-up(P=0.813). There was no significant difference in operation time between two groups(P>0.05). There were significant differences between two groups in fluoroscopy times, guide needle drilling times, hollow screw replacement times, and intraoperative bleeding volume(P<0.05). CONCLUSION: Closed reduction and hollow screw internal fixation assisted by robot navigation system for femoral neck fracture has the advantages of minimally invasive operation, precise screw placement, and reduction of X-ray radiation damage during operation.


Assuntos
Fraturas do Colo Femoral , Ortopedia , Robótica , Masculino , Feminino , Humanos , Adulto , Pessoa de Meia-Idade , Idoso , Resultado do Tratamento , Fraturas do Colo Femoral/cirurgia , Parafusos Ósseos , Fixação Interna de Fraturas , Consolidação da Fratura , Estudos Retrospectivos
5.
Nat Sci Sleep ; 16: 85-97, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38333420

RESUMO

Objective: Weekend sleep duration is linked to health issues, including mortality. However, how weekend sleep duration can impact chronic kidney disease (CKD) still needs to be understood. Therefore, we aimed to analyze how weekend sleep duration is associated with kidney function. Methods: This is a cross-sectional study. Data were obtained from the 2017-2018 National Health and Nutrition Examination Survey. We included 5362 study participants and categorized them into nine subgroups by sleep duration (short: ≤6 hours, normal: 6-9 hours, and long: ≥9 hours) on weekdays and weekends and analyzed for the respective association with renal function using stratified multivariable linear regression. Results: Weekend sleep duration for 9 hours or more was associated with decreasing estimated glomerular filtration rate (eGFR) levels by 2.8 to 6.4 mL/min/1.73 m2 among people with long to short weekday sleep duration compared with short weekday and weekend sleep durations (control group) after adjusting for demographic characteristics, body measurement, sleep quality, smoking, and comorbidities. The study population with short weekday sleep duration (sWK) and long weekend sleep duration (lWD) had the most significant decline in eGFR. For the study population with sWK, eGFR level significantly decreased by 1.1 mL/min/1.73 m2 as sleep duration on weekends increased by one hour. Conclusion: The underlying mediators of lWD and CKD could be the dysregulation of human behaviors, metabolism, or biological functions. Longer weekend sleep duration was linked to a decrease in eGFR levels. It warrants further study to clarify the mediators.

6.
ACS Sens ; 9(2): 577-588, 2024 02 23.
Artigo em Inglês | MEDLINE | ID: mdl-38254273

RESUMO

Revolutionary developments in analytical chemistry have led to the rapid development of self-powered photoelectrochemical (PEC) sensors. Different from conventional PEC sensors, self-powered PEC sensors do not require an external power source or complex devices for the sensitive detection of targets. As a result, these sensors have enormous application potential for the development of novel portable sensors. An increasing body of work is making excellent progress toward the implementation of self-powered PEC sensors for detection, but there have been no reviews to date. The present review first introduces the state of the art in the development of self-powered PEC sensors. Then, different types of self-powered PEC sensors are summarized and discussed in detail, including their current, power, and potential. Additionally, single- and dual-photoelectrode systems are classified and systematically compared. Finally, the current developments and major challenges that need to be addressed are also summarized. This review provides valuable insights into the current state of self-powered PEC sensors to promote further progress in this field.


Assuntos
Técnicas Biossensoriais , Fontes de Energia Elétrica
7.
JMIR Form Res ; 8: e43286, 2024 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-38294871

RESUMO

BACKGROUND: Irritable bowel syndrome (IBS) is a disorder of the gut-brain interaction that is associated with abdominal pain, altered bowel patterns, and reduced quality of life. Up to 50% of patients with IBS also report anxiety or depressive symptoms. Although effective self-management interventions exist for individuals with IBS, few have been effectively implemented, and most do not consider the unique needs of patients with comorbid IBS and anxiety or depression. OBJECTIVE: This study aimed to determine the anticipated acceptability, appropriateness, feasibility, and usability of a comprehensive self-management intervention using an implementation science and human-centered design approach among individuals with comorbid IBS and anxiety or depression and health care providers. METHODS: A convergent mixed methods design was used to elicit feedback on the comprehensive self-management intervention outline and content to identify refinement needs before testing. Patients with IBS and moderate to severe anxiety or depression and health care providers were purposefully sampled from primary care and gastroenterology settings. Participants completed semistructured interviews and surveys on anticipated acceptability, appropriateness, feasibility, and usability. RESULTS: Patient participants (n=12) were on average 36.8 (SD 12.2) years of age, and 42% (5/12) were currently receiving psychological therapy. Health care providers (n=14) were from primary care (n=7) and gastroenterology (n=7) settings. The mean usability scores (out of 100) were 52.5 (SD 14.5) for patients and 45.6 (SD 11.6) for providers. For patients and providers, qualitative data expanded the quantitative findings for acceptability and appropriateness. Acceptability findings were the comprehensive nature of the intervention and discussion of the gut-brain interaction. For appropriateness, participants reported that the intervention provided structure, accountability, and support. Feasibility was confirmed for patients, but there was a divergence of findings between quantitative and qualitative measures for providers. Patients focused on intervention feasibility, while providers focused on implementation feasibility in the clinic. Identified usability issues to address before implementation included the intervention delivery format, length, and lack of integration into health care settings that, if not addressed, may limit the reach of the intervention. CONCLUSIONS: Patients and health care providers found the intervention acceptable and appropriate. Several feasibility and usability issues were identified, including intervention delivery methods, length of intervention, and the best methods to implement in the clinic setting. The next steps are to refine the intervention to address the identified issues and test in a pilot study whether addressing usability issues leads to the anticipated improvements in implementation and uptake.

8.
J Inorg Biochem ; 251: 112434, 2024 02.
Artigo em Inglês | MEDLINE | ID: mdl-38029537

RESUMO

A series of novel dibutyltin complexes based on salen-like ligands (S01-S03) were synthesized and characterized using ultraviolet-visible spectra,infrared spectra, 1H, 13C, and 119Sn nuclear magnetic resonance, high-resolution mass spectrometry, X-ray crystallography, and thermogravimetric analysis. Complex S03 had excellent anticancer activity in vitro (IC50 = 1.5 ± 0.2 µM in CAL-27 cell lines), which highly activated ROS expression levels and induced apoptosis and cell cycle arrest at the G2/M phase. Interestingly, complex S03 induced cancer cell death through multiple mechanisms (mitochondrial pathway, ER-stress pathway, and DNA damage pathway). This study reveals new mechanisms of organotin complexes and provides new insights into the development of organotin metal complexes as anticancer drugs in the future, and compounds with multiple anticancer mechanisms may be a new strategy for delaying or overcoming drug resistance to chemotherapy and target therapy.


Assuntos
Antineoplásicos , Complexos de Coordenação , Compostos Orgânicos de Estanho , Compostos Orgânicos de Estanho/química , Antineoplásicos/química , Etilenodiaminas/farmacologia , Complexos de Coordenação/química , Linhagem Celular Tumoral , Apoptose , Ligantes
9.
J Ethnopharmacol ; 323: 117667, 2024 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-38159821

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: The Stephania cephalantha Hayata is an important traditional medicinal plant widely used in traditional medicine to treat cancer. Cepharanthine (CEP) was extracted from the roots of Stephania cephalantha Hayata. It has been found to exhibit anticancer activity in different types of cancer cells. Nevertheless, the activity of CEP against nasopharyngeal carcinoma (NPC) and its underlying mechanism warrant further investigation. AIMS OF THE STUDY: NPC is an invasive and highly metastatic malignancy that affects the head and neck region. This research aimed to investigate the pharmacological properties and underlying mechanism of CEP against NPC, aiming to offer novel perspectives on treating NPC using CEP. MATERIALS AND METHODS: In vitro, the pharmacological activity of CEP against NPC was evaluated using the CCK-8 assay. To predict and elucidate the anticancer mechanism of CEP against NPC, we employed network pharmacology, conducted molecular docking analysis, and performed Western blot experiments. In vivo validation was performed through a nude mice xenograft model of human NPC, Western blot and immunohistochemical (IHC) assays to confirm pharmacological activity and the mechanism. RESULTS: In a dose-dependent manner, the proliferation and clonogenic capacity of NPC cells were significantly inhibited by CEP. Additionally, NPC cell migration was suppressed by CEP. The results obtained from network pharmacology experiments revealed that anti-NPC effect of CEP was associated with 8 core targets, including EGFR, AKT1, PIK3CA, and mTOR. By performing molecular docking, the binding capacity of CEP to the candidate core proteins (EGFR, AKT1, PIK3CA, and mTOR) was predicted, resulting in docking energies of -10.0 kcal/mol for EGFR, -12.4 kcal/mol for PIK3CA, -10.8 kcal/mol for AKT1, and -8.6 kcal/mol for mTOR. The Western blot analysis showed that CEP effectively suppressed the expression of EGFR and the phosphorylation levels of downstream signaling proteins, including PI3K, AKT, mTOR, and ERK. After CEP intervention, a noteworthy decrease in tumor size, without inducing any toxicity, was observed in NPC xenograft nude mice undergoing in vivo treatment. Additionally, IHC analysis demonstrated a significant reduction in the expression levels of EGFR and Ki-67 following CEP treatment. CONCLUSION: CEP exhibits significant pharmacological effects on NPC, and its mechanistic action involves restraining the activation of the EGFR/PI3K/AKT pathway. CEP represents a promising pharmaceutical agent for addressing and mitigating NPC.


Assuntos
Benzodioxóis , Benzilisoquinolinas , Neoplasias Nasofaríngeas , Proteínas Proto-Oncogênicas c-akt , Stephania , Animais , Camundongos , Humanos , Carcinoma Nasofaríngeo/tratamento farmacológico , Carcinoma Nasofaríngeo/metabolismo , Simulação de Acoplamento Molecular , Camundongos Nus , Proteínas Proto-Oncogênicas c-akt/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Farmacologia em Rede , Linhagem Celular Tumoral , Serina-Treonina Quinases TOR/metabolismo , Neoplasias Nasofaríngeas/tratamento farmacológico , Neoplasias Nasofaríngeas/metabolismo , Classe I de Fosfatidilinositol 3-Quinases/uso terapêutico , Receptores ErbB
10.
J Ethnopharmacol ; 319(Pt 3): 117327, 2024 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-37871755

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Litchi chinensis Sonn. (Litchi) seed, a traditional Chinese medicine, is habitually used in the clinical treatment of prostate cancer (PCa)-induced bone pain. In our previous study, flavonoids have been identified as the active ingredient of litchi seed against PCa. However, its anti-tumor activities in bone and associated molecular mechanisms are still unclear. AIM OF THE STUDY: To investigate the effects and underlying mechanisms of total flavonoids of litchi seed (TFLS) on the growth of PCa in bone. MATERIALS AND METHODS: The effect of TFLS on the growth of PCa in bone was observed using a mouse model constructed with tibial injection of luciferase-expressing RM1-luc cells. Conditioned medium (CM) from bone marrow stromal cells OP9 and CM treated with TFLS (T-CM) was used to investigate the effect on the proliferation, colony formation, and apoptosis of PCa cells (LNCaP, PC3, RM1). An antibody microarray was performed to detect cytokine expression in the supernatant fraction of OP9 cell cultures treated with TFLS or left untreated. Western blot assay was employed to determine the expression and activity of HGFR and its key downstream proteins, Akt, mTOR, NF-κB, and Erk, in PCa cells. The potential target was further verified using immunofluorescence and immunohistochemistry assays. RESULTS: Treatment with TFLS (80 mg/kg, 24 days) significantly suppressed the growth of RM1 cells in bone. CM from bone marrow stromal cells OP9 stimulated the proliferation and colony formation of the PCa cells as well as inhibited the apoptosis of PC3 cells, while T-CM reversed the effects mediated by OP9 cells in vitro. In an antibody array assay, TFLS regulated the majority of cytokines in OP9 cell culture supernatant, among which HGF, HGFR, IGF-1R, and PDGF-AA showed the greatest fold changes. Mechanistically, CM upregulated HGFR and promoted phosphorylation of NF-κB while T-CM induced reduction of HGFR and dephosphorylation of NF-κB in PC3 cells. Moreover, T-CM inhibited NF-κB entry into PC3 cell nuclei. Data from in vivo experiments further confirmed the inhibitory effects of TFLS on NF-κB. CONCLUSION: TFLS suppresses the growth of PCa in bone through regulating bone microenvironment and the underlying mechanism potentially involves attenuation of the HGFR/NF-κB signaling axis.


Assuntos
Litchi , Neoplasias da Próstata , Masculino , Humanos , NF-kappa B/metabolismo , Litchi/química , Litchi/metabolismo , Flavonoides/farmacologia , Flavonoides/uso terapêutico , Transdução de Sinais , Neoplasias da Próstata/metabolismo , Citocinas/farmacologia , Linhagem Celular Tumoral , Microambiente Tumoral
11.
Environ Sci Pollut Res Int ; 30(43): 97195-97208, 2023 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-37589843

RESUMO

Lincomycin (LC) is an extensively applied broad-spectrum antibiotic, and its considerable residues in wastewater have caused a series of environmental problems, which makes degradation of LC wastewater extremely urgent. In this work, we have constructed a novel boron nitride (BN) and samarium (Sm) co-modified Ti/PbO2 as anode for high-performance degradation of LC wastewater. Compared with Ti/PbO2, Ti/PbO2-Sm, and Ti/PbO2-BN electrodes, Ti/PbO2-BN-Sm electrode with smaller pyramidal particles possesses higher oxygen evolution potential (2.32 V), excellent accelerated service life (103 h), and outstanding electrocatalytic activity. The single-factor experiments demonstrate that under optimized conditions (current density of 20 mA.cm-2, 6.0 g L-1 Na2SO4, pH 9, and temperature of 30°C), removal rate and COD degradation rate of LC at 3 h have reached 92.85% and 89.11%, respectively. At the same time, degradation of LC is in accordance with the primary kinetic model. Based on the analysis of high-performance liquid chromatography-mass spectrometry (HPLC-MS), four possible degradation pathways are hypothesized. Therefore, efficient electrochemical degradation of LC by using an extremely long-life Ti/PbO2 electrode with high catalytic activity may be a promising method.


Assuntos
Lincomicina , Samário , Águas Residuárias , Galvanoplastia , Titânio , Eletrodos
12.
Am J Gastroenterol ; 118(9): 1648-1655, 2023 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-37040543

RESUMO

INTRODUCTION: Irritable bowel syndrome (IBS) is a common disorder of gut-brain interaction, characterized by symptoms of abdominal pain and changes in bowel habits. It often co-occurs with extraintestinal somatic and psychological symptoms. However, the nature of the interrelationships among these symptoms is unclear. Although previous studies have noted age differences in IBS prevalence and specific symptom severity, it remains unknown whether specific symptoms and symptom relationships may differ by age. METHODS: Symptom data were collected in 355 adults with IBS (mean age 41.4 years, 86.2% female). Network analysis was used to examine the interrelationships among 28 symptoms and to identify the core symptoms driving the symptom structure between young (≤45 years) vs older (>45 years) adults with IBS. We evaluated 3 network properties between the 2 age groups: network structure, edge (connection) strength, and global strength. RESULTS: In both age groups, fatigue was the top core symptom. Anxiety was a second core symptom in the younger age group, but not the older age group. Intestinal gas and/or bloating symptoms also exerted considerable influences in both age groups. The overall symptom structure and connectivity were found to be similar regardless of age. DISCUSSION: Network analysis suggests fatigue is a critical target for symptom management in adults with IBS, regardless of age. Comorbid anxiety is likely an important treatment focus for young adults with IBS. Rome V criteria update could consider the importance of intestinal gas and bloating symptoms. Additional replication with larger diverse IBS cohorts is warranted to verify our results.


Assuntos
Síndrome do Intestino Irritável , Adulto Jovem , Humanos , Feminino , Idoso , Adulto , Masculino , Síndrome do Intestino Irritável/complicações , Síndrome do Intestino Irritável/epidemiologia , Síndrome do Intestino Irritável/diagnóstico , Defecação , Ansiedade/epidemiologia , Comorbidade , Dor Abdominal/epidemiologia , Dor Abdominal/etiologia , Inquéritos e Questionários
13.
Anal Chem ; 95(18): 7303-7311, 2023 05 09.
Artigo em Inglês | MEDLINE | ID: mdl-37096866

RESUMO

Exploiting ingenious photoelectrodes and innovative signal amplification strategies has the potential to achieve high sensitivity in self-powered cathodic photoelectrochemical (PEC) analysis. In this work, a novel self-powered PEC sensing platform was constructed by integrating a synergistic signal amplification of an ingenious photocathode with a high light-harvesting photoanode. In the dual photoelectrode-based PEC system, the amplified photocurrent signals were induced by a synergistic enhancement: (1) the photocurrent of the BiOBr photocathode was improved by the incorporation of nitrogen-doped graphene; (2) the photocurrent of the self-powered sensor was activated by the high-light-harvesting Bi2S3-C3N4 photoanode. Subsequently, the rational mechanism for synergistic signal amplification was investigated. For the construction of the sensing interface, an aptamer was introduced as the recognition element to specifically capture the streptomycin (STR) target. Under optimal conditions, the constructed self-powered aptasensor has the merits of good linear range (1 × 10-11 to 5 × 10-7 M), acceptable limit of detection (1.18 × 10-12 M), and excellent stability and selectivity for STR detection. Additionally, the proposed self-powered aptasensor showed acceptable accuracy for the detection of STR in water. Hopefully, this might stimulate more interest in designing and constructing novel platforms for exquisite photocathodic monitoring of various contaminants in the environment.


Assuntos
Aptâmeros de Nucleotídeos , Técnicas Biossensoriais , Grafite , Técnicas Eletroquímicas , Eletrodos , Limite de Detecção
14.
J Chromatogr A ; 1694: 463909, 2023 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-36893507

RESUMO

Identifying and quantifying polymeric impurities in a polymeric material is critical for understanding material quality and performance, but it remains a challenge requiring developing new characterization methods. In this work, a comprehensive two-dimensional liquid chromatography method with simultaneous evaporative light scattering and high-resolution mass spectrometry detection was developed to separate and identify a polymeric impurity in alkyl alcohol-initiated polyethylene oxide/polybutylene oxide diblock copolymer. Size exclusion chromatography was implemented in the first dimension, and gradient reversed-phase liquid chromatography using a large-pore C4 column was applied in the second dimension using an active solvent modulation valve as the interface to minimize the polymer breakthrough. The two-dimensional separation significantly reduced the complexity of the mass spectra data compared to the one-dimensional separation, and the combination of retention time and mass spectral interpretation led to the successful identification of the water-initiated triblock copolymer impurity. This identification was confirmed by comparison with the synthesized triblock copolymer reference material. A one-dimensional LC method with evaporative light scattering detection was employed to quantify the triblock impurity. The impurity level in three samples made with the different processes was determined to be in the range of 9-18 wt% using the triblock reference material as the standard.


Assuntos
Polietilenoglicóis , Polímeros , Polímeros/química , Espectrometria de Massas , Cromatografia em Gel , Polietilenoglicóis/química , Cromatografia de Fase Reversa/métodos , Cromatografia Líquida de Alta Pressão/métodos
15.
Environ Sci Pollut Res Int ; 30(18): 52107-52123, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-36826770

RESUMO

For the first time, N-doped carbon materials with 3D porous-layered skeleton structure was synthesized through a one-step co-pyrolysis method, which was fabricated by co-pyrolysis of natural corn starch and melamine using metal catalysts (Ni (II) and Mn (II)). The 3D-NC possessed a heterogeneously meso-macroporous surface with a hierarchically connected sheet structure inside. Batch adsorption experiments suggested that highly selective adsorption of Hg (II) by the 3D-NC could be completed within 90 min and had maximum adsorption capacities as high as 403.24 mg/g at 293 K, pH = 5. The adsorption mechanism for Hg (II) was carefully evaluated and followed the physical adsorption, electrostatic attraction, chelation, and ion exchange. Besides, thermodynamic study demonstrated that the Hg (II) adsorption procedure was spontaneous, endothermic, and randomness. More importantly, the 3D-NC could be regenerated and recovered well after adsorption-desorption cycles, showing a promising prospect in the remediation of Hg (II)-contaminated wastewater.


Assuntos
Mercúrio , Poluentes Químicos da Água , Carbono , Adsorção , Porosidade , Amido , Poluentes Químicos da Água/análise , Mercúrio/análise , Água , Cinética
16.
Biol Res Nurs ; 25(1): 5-13, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-35759356

RESUMO

Background: Survivors of acute respiratory failure (ARF) experience long-term cognitive impairment and circadian rhythm disturbance after hospital discharge. Although prior studies in aging and neurodegenerative diseases indicate actigraphy-estimated rest-activity circadian rhythm disturbances are risk factors for cognitive impairment, it is unclear if this applies to ARF survivors. This study explored the relationships of actigraphy-estimated rest-activity circadian rhythms with cognitive functioning in ARF survivors at 3 months after discharge. Methods: 13 ARF survivors (mean age 51 years and 69% males) completed actigraphy and sleep diaries for 9 days, followed by at-home neuropsychological assessment. Principal component factor analysis created global cognition and circadian rhythm variables, and these first components were used to examine the global relationships between circadian rhythm and cognitive measure scores. Results: Global circadian function was associated with global cognition function in ARF survivors (r = .70, p = .024) after adjusting for age, education, and premorbid cognition. Also, greater fragmented rest-activity circadian rhythm (estimated by intradaily variability, r = .85, p = .002), and weaker circadian strength (estimated by amplitude, r = .66, p = .039; relative strength, r = .70, p = .024; 24-h lag serial autocorrelation, r = .67, p = .035), were associated with global cognition and individual cognitive tests. Conclusions: These results suggest circadian rhythm disturbance is associated with poorer global cognition in ARF survivors. Future prospective research with larger samples is needed to confirm these results and increase understanding of the relationship between disrupted circadian rhythms and cognitive impairment among ARF survivors.


Assuntos
Disfunção Cognitiva , Insuficiência Respiratória , Masculino , Humanos , Pessoa de Meia-Idade , Feminino , Sono , Actigrafia , Ritmo Circadiano , Disfunção Cognitiva/etiologia
17.
J Colloid Interface Sci ; 632(Pt A): 35-43, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36403375

RESUMO

The development of effective strategies for the detection of oxytetracycline (OTC) in soil is of great importance for preserving agri-environmental safety and human health. Herein, a novel photoactive material of metal-organic framework (MOF) derived ZnO nanopolyhedra/graphitic carbon nitride (ZnO/g-C3N4) heterojunction was designed by mixing calcination of zeolite imidazole framework-8 (ZIF-8) and melamine. A self-powered photoelectrochemical aptasensor for the sensitive and selective detection of OTC in soil was proposed using ZnO/g-C3N4 as the photoanode. The photoactivity of the MOF derived ZnO nanopolyhedra was regulated effectively by the introduction of g-C3N4, which resulted in a 7-fold increase in the photocurrent of the ZnO nanopolyhedra at a bias potential of 0 V. It was assigned to the higher carrier density of ZnO/g-C3N4. By virtue of the amplified photocurrent of ZnO/g-C3N4, the specificity of the OTC aptamer and the anti-interference ability of the self-powered sensing method, the designed aptasensor demonstrated the advantages of a wide linear range (0.005-200 nM), low limit of detection (1.49 × 10-3 nM), good selectivity and good reproducibility. For real soil sample analysis, satisfactory recoveries were obtained and further verified by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).

18.
Front Cell Infect Microbiol ; 12: 1057492, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36439220

RESUMO

Background: Fecal microbiota transplantation (FMT) is an emerging therapy for diseases associated with intestinal flora imbalance that has attracted increasing attention in recent years. This study aims to provide an overview of research trends in the field, and act as a reference point for future scientific research by analyzing the state of current research, identifying hotspots, and potential frontiers of FMT. Methods: Articles relating to FMT that were published between the years 2012 and 2021 were retrieved from the Web of Science Core Collection. Bibliometric analysis was performed using Microsoft Excel and CiteSpace. Results: A total of 2,403 English language articles relating to FMT research were published over the last ten years. Most of this research was carried out in the United States of America, with Harvard Medical school being the most productive institution. Much of the research was published in the PLoS One journal. Alexander Khoruts was identified as a prominent, productive researcher in the field. Keyword analysis revealed that research hot spots included gut microbiota, Clostridium difficile infection (CDI), and diseases. Burst detection indicated that future research frontiers include clinical practice guidelines and strategies. Conclusion: Our analysis explored hot spots and emerging trends in the FMT field. Indications for use of FMT extended from digestive system diseases to other systemic diseases. Additionally, areas such as risk assessment and control, along with application methods were also a focus of current research. Moreover, research relating to optimization of clinical practice has excellent prospects.


Assuntos
Dermatite , Enterocolite Pseudomembranosa , Microbioma Gastrointestinal , Humanos , Transplante de Microbiota Fecal , Bibliometria , Clorexidina
19.
Biosens Bioelectron ; 216: 114634, 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36049349

RESUMO

Efficient and rapid detection methods for antibiotics are of great practical importance to achieve environmental safety and protect human health. PEC sensors have been widely employed in antibiotics analysis due to their advantages of low background signal, high sensitivity, simple operation, and wide dynamic response range. This review is focused on the recent progress in photoactivities regulating of inorganic semiconductors from metal oxides/sulfides, metal-based quantum dots to metal-free carbon nitride and various types of PEC sensors in antibiotics monitoring which covers sensing strategies without or with a recognition element (antibody, molecularly imprinted polymer and aptamer). Finally, the challenges and the development prospects of the PEC sensors for antibiotics detection are discussed.


Assuntos
Técnicas Biossensoriais , Técnicas Eletroquímicas , Antibacterianos , Técnicas Biossensoriais/métodos , Técnicas Eletroquímicas/métodos , Humanos , Polímeros Molecularmente Impressos , Óxidos , Semicondutores , Sulfetos
20.
Gastroenterology ; 163(4): 995-1023.e3, 2022 10.
Artigo em Inglês | MEDLINE | ID: mdl-35716771

RESUMO

BACKGROUND & AIMS: Many studies have assessed risk factors of irritable bowel syndrome (IBS) and other abdominal pain-related disorders of gut-brain interaction (AP-DGBI); however, the role of these factors is unclear due to heterogeneous study designs. The aim of this systematic review was to extensively evaluate the literature and determine clinical risk and protective factors for the presence and persistence of AP-DGBI in children and adults. METHODS: A PubMed search identified studies investigating potential risk and protective factors for AP-DGBI in adults and children. Inclusion criteria included fully published studies with a control group; exclusion criteria included poor-quality studies (using a validated scale). For each factor, the proportion of studies that found the factor to be a risk factor, protective factor, or neither was summarized. The number of studies, diagnostic criteria, number of subjects, and average study quality rating provided further context. Whenever possible, a meta-analysis generated pooled odds ratios or mean difference. RESULTS: The systematic review included 348 studies. Female sex, gastroenteritis, abuse, stress, psychological disorders, somatic symptoms, and poor sleep were consistent risk factors for developing AP-DGBI in adults and children. In adults, additional risk factors included obesity, smoking, and increased use of medical resources. Protective AP-DGBI factors in adults included social support and optimism; no studies for protective factors were found for children. CONCLUSIONS: There are multiple risk factors for AP-DGBI in adults and children. These include female sex, gastroenteritis, abuse, stress, poor sleep, obesity, psychological disorders, and somatic symptoms. Additional studies are needed in children, on protective factors, and on factors associated with persistence of AP-DGBI.


Assuntos
Gastroenterite , Gastroenteropatias , Síndrome do Intestino Irritável , Sintomas Inexplicáveis , Dor Abdominal/epidemiologia , Dor Abdominal/etiologia , Adulto , Encéfalo , Criança , Feminino , Gastroenterite/complicações , Gastroenteropatias/diagnóstico , Gastroenteropatias/epidemiologia , Gastroenteropatias/etiologia , Humanos , Síndrome do Intestino Irritável/complicações , Obesidade/complicações , Fatores de Risco
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