The restricted mass transfer inside the anode pore channel affects the electroactive biofilms formation, community composition and the power production in microbial electrochemical systems.

Porous anodes improve system performance in microbial electrochemical systems by increasing the specific surface area for electroactive bacteria. In this study, multilayer anodes with different pore diameters were constructed to assess the impact of pore size and depth on anode performance. This layered structure makes detecting electroactive biofilms more accessible layer by layer, which is the first study to examine electroactive biofilms' molecular biology and electrochemical properties at different depths in pores with varied pore sizes. The millimeter-scale pores inside the bioanode have a limited effect in increasing power. The larger the pore diameter, the higher the maximum power density (Pmax) obtained. The Pmax of anodes with 4 mm pore (1.91 ± 0.15 W m-2) was 1.4 times higher than that of the non-perforated (1.37 ± 0.07 W m-2) and 0.5 mm pore anodes (1.39 ± 0.04 W m-2). Electricigens can colonize into pore channels for at least 10 mm with a pore diameter ≥3 mm and current densities >0.05 A m-2. However, in the pores channel with 0.5 mm diameter, electricigens can only colonize to a depth of 2 mm. The biofilm thickness, electricity output, metabolic activity, and biocommunity changed with pore depth and were restricted by the limited mass transfer. The Geobacter sp. was the dominant species in inter-pore biofilms, with 43.8 %-78.6 % in abundance and decreased in quantity as pore depth increased. The inter-pore biofilms on the outer layer contributed a current density of 0.17 ± 0.003 A m-2, while that of the inner layer was only 0.02 ± 0.01 A m-2. Further studies found that the pore edge mass transfer effect can contribute up to 75 % of the current. The mass transfer process at the pore edge region could be a multidirectional mass transfer rather than a pore channel mass transfer.

Liquid-gas phase transition enables microbial electrolysis and H2-based membrane biofilm hybrid system to degrade organic pollution and achieve effective hydrogenotrophic denitrification of groundwater.

Electron-donor Lacking was the limiting factor for the denitrification of oligotrophic groundwater and hydrogenotrophic denitrification provided an efficient approach without secondary pollution. In this study, a hybrid system with microbial electrolysis cell (MEC) assisted hydrogen-based membrane biofilm reactor (MBfR) was established for advanced groundwater denitrification. The liquid-gas phase transition prevented the potential pollution from organic wastes in MEC to groundwater, while the bubble-free diffusion of MBfR promoted hydrogen utilization efficiency. The negative-pressure extraction from MEC and the positive pressure for gas supply into MBfR increased the hydrogen proportion and current density of MEC, and improved the kinetic constant K of the denitrification reaction in MBfR. With actual groundwater, the MEC-MBfR hybrid system achieved a nitrate reduction of 97.8% with an effluent NO3--N of 2.2 ± 1.0 mg L-1. The hydrogenotrophic denitrifiers of Thauera, Pannonibacter, and Azonexus, dominated the denitrification biofilm on the membrane and elastic filler in MBfR.

Lignocellulose reconstituted shape-controllable self-supporting carbonaceous capacitance-anodes with high electron transfer rates for high-performance microbial electrochemical system.

Natural biomass is a promising candidate for manufacturing an efficient anode in the microbial electrochemical system (MES) for its abundance and low cost. However, the structure and performance of the electrode highly depend on the biomass species. A simple and sustainable method for creating a self-supporting electrode is proposed by freeze-drying and carbonizing a blend of cellulose, lignin, and hemicellulose. This strategy leads to a cork-like structure and improved mechanical strength of the lignocellulose carbon. A power density of 4780 ± 260 mW m-2 (CLX-800) was achieved, which was the highest record for unmodified lignocellulose-based anodes in the microbial fuel cells. The morphological as lamellar multilayer and rich in hydrophilic functional groups could facilitate the formation of thick electroactive biofilms and enrich Geobacter with the highest abundance of 92.3%. The CLX material is expected to be the ideal electrode for high performance and functionally controllability.

A three-dimensional phenolic-based carbon anode for microbial electrochemical system with customized macroscopic pore structure to promote interior bacteria colonization.

Microbial electrochemical system (MES) is an emerging wastewater treatment technology that compensates the energy demands of containments removal by in situ converting the chemical energy of organic pollutants. As the structure for exoelectrogens and the reaction site of extracellular electron transfer (EET), the anode is essential for MES. The future commercial application of MES requires efficiency and large-scale fabrication available anode. In this study, a 3D anode with millimeter-scale pores (3D-MPA) was successfully constructed by sacrificial template method, with low-cost phenolic resin as carbon precursor and polymethyl methacrylate (PMMA) pellets as template. With customized and ordered pore of 1 mm, the 3D-MPAs allowed the microorganisms to colonize inside, improving anodic space utilization efficiency. Different carbonization temperature in tested range from 700 °C to 1000 °C regulated the micrometer-scale convex structures and surface roughness of 3D-MPAs, causing electrochemical performance changes. The 3D-MPA-900 obtained the largest electroactive surface area (102 ± 4.1 cm2) and smallest ohmic resistance (1.8 ± 0.09 Ω). Equipped with MES, 3D-MPA-900 reached the highest power density and current density (2590 ± 25 mW m-2 and 5.20 ± 0.07 A m-2). Among tested 3D-MPA, the excellent performance of 3D-MPA-900 might be attributed by its convex structures with suitable size and surface coverage. The surface roughness of 3D-MPA-900 enhanced the microorganism adherence, which then promoted EET on anode surface. Generally, phenolic-based 3D-MPA made of sacrificial-template method had controllable porous structure, large-scale fabrication availability, high chemical stability and excellent mechanical property, which could be promising for the commercial application of MES.

Conversion from rice fields to vegetable fields alters product stoichiometry of denitrification and increases N2O emission.

Information about effects of conversion from rice fields to vegetable fields on denitrification process is still limited. In this study, denitrification rate and product ratio (i.e., N2O/(N2O + N2) ratio) were investigated by soil-core incubation based N2/Ar technique in one rice paddy field (RP) and two vegetable fields (VF4 and VF7, 4 and 7 years vegetable cultivating after conversion from rice fields, respectively). Genes related to denitrification and bacterial community composition were quantified to investigate the microbial mechanisms behind the effects of land-use conversion. The results showed that conversion of rice fields to vegetable fields did not significantly change denitrification rate although the abundance of denitrification related genes was significantly reduced by 79.22%-99.84% in the vegetable soils. Whereas, compared with the RP soil, N2O emission rate was significantly (P < 0.05) increased by 53.5 and 1.6 times in the VF4 and VF7 soils, respectively. Correspondingly, the N2O/(N2O + N2) ratio increased from 0.18% (RP soil) to 5.65% and 0.65% in the VF4 and VF7 soils, respectively. These changes were mainly attributed to the lower pH, higher nitrate content, and the altered bacterial community composition in the vegetable soils. Overall, our results showed that conversion of rice fields to vegetable fields increased the N2O emission rate and altered the product ratio of denitrification. This may increase the contribution of land-use conversion to global warming and stratospheric ozone depletion.

Economic affordable carbonized phenolic foam anode with controlled structure for microbial fuel cells.

In microbial fuel cells (MFCs), the anode electrode is a core structure as the catalytic area of exoelectrogens. The anode material for large-scale MFCs needs excellent bioelectrochemical performance and low fabrication costs. Herein, carbonized phenolic foam with controllable porous structures was developed as the bio-capacitor of MFCs. The proportion of sodium dodecylbenzene sulfonate (SDBS), which improved mixing and dissolution between the resin liquid and the foaming agent, was adjusted to form open pores on the foam film and skeletons, which promoted both the capacitance and biocompatibility of the anode. Within SDBS proportion from 0 to 1.2 wt%, the anode SPF-9 (0.9 wt%) obtained the best capacitance (37 ±â€¯0.13 F g-1), electrochemical active surface area (87 ±â€¯0.38 cm2) and hydrophilia (contact angle 79 ±â€¯0.2°). The MFCs with SPF-9 obtained the highest power density of 3980 ±â€¯178 mW m-2, while those of carbon-cloth anodes were 1600 ±â€¯28 mW m-2. The biofilm of SPF-9 also demonstrated higher activity and obtained larger abundance of exoelectrogens (68 ±â€¯0.38%). The increased capacitance and biocompatibility mainly resulted in the good performance of SPF-9. The carbonized phenolic foam anode material was worth considering for the future application of MFCs due to its superior electrochemical performance and large-quantity fabrication capability.

Tetracycline and sulfamethazine alter dissimilatory nitrate reduction processes and increase N2O release in rice fields.

Effects of antibiotics on the transformation of nitrate and the associated N2O release in paddy fields are obscure. Using soil slurry experiments combined with 15N tracer techniques, the influence of tetracycline and sulfamethazine (applied alone and in combination) on the denitrification, anaerobic ammonium oxidation (anammox), dissimilatory nitrate reduction to ammonium (DNRA) and N2O release rates in the paddy soil were investigated, while genes related to nitrate reduction and antibiotic resistance were quantified to explore the microbial mechanisms behind the antibiotics' effects. The potential rates of denitrification, anammox, and DNRA were significantly (p < 0.05) reduced, which were mainly attributed to the inhibitory effects of the antibiotics on nitrate-reducing microbes. However, the N2O release rates were significantly (p < 0.05) stimulated by the antibiotic treatments (0.6-6000 µg kg-1 soil dry weight), which were caused by the different inhibition effects of antibiotics on N2O production and N2O reduction as suggest by the changes in abundance of nirS (nitrite reduction step) and nosZ (N2O reduction to N2 step) genes. Antibiotic resistance gene (tetA, tetG, sulI, and sulIII) abundances were significantly (p < 0.05) increased under high antibiotic exposure concentrations (>600 µg kg-1 soil dry weight). Our results suggest that the widespread occurrence of antibiotics in paddy soils may pose significant eco-environmental risks (nitrate accumulation and greenhouse effects) by altering nitrate transformation processes.

Effects of long-term pig manure application on antibiotics, abundance of antibiotic resistance genes (ARGs), anammox and denitrification rates in paddy soils.

Previous studies of long-term manure applications in paddy soil mostly focused on the effects on denitrification, occurrence of antibiotics and antibiotic resistance genes (ARGs) without considering the effects on anaerobic ammonium oxidation (anammox). Here, we investigated the potential rates of anammox and denitrification, occurrence of antibiotics and AGRs in response to three fertilization regimes (C, no fertilizer; N, mineral fertilizer; and NM, N plus pig manure) in six long-term paddy experiment sites across China. The potential rates of anammox (0.11-3.64 nmol N g-1 h-1) and denitrification (1.5-29.05 nmol N g-1 h-1) were correlated with the abundance of anammox genes (hzsB) and denitrification functional genes (narG, nirK, nirS and nosZ), respectively. The anammox and denitrification rates were affected by soil organic carbon (SOC) and significantly (p < 0.05) increased in NM treatments relative to those in N treatments. Although pig manure application increased antibiotic concentrations and abundance of ARGs compared with N treatments, the increased antibiotics did not directly affect the anammox and denitrification rates. Our results suggested that long-term pig manure application significantly increased antibiotic concentrations, abundance of ARGs, and rates of anammox and denitrification, and that the effects of pig manure-derived antibiotics on anammox and denitrification were marginal. This is the first report that investigates the effects of long-term pig manure application on anammox in paddy soils. More attention should be paid to the potential ecological risk of increased ARGs caused by pig manure application in paddy soils.