Nuclear factors NF-YC3 and NF-YBs positively regulate arbuscular mycorrhizal symbiosis in tomato.

The involvement of nuclear factor Y (NF-Y) in transcriptional reprogramming during arbuscular mycorrhizal symbiosis has been demonstrated in several plant species. However, a comprehensive picture is lacking. We showed that the spatial expression of NF-YC3 was observed in cortical cells containing arbuscules via the cis-regulatory element GCC boxes. Moreover, the NF-YC3 promoter was transactivated by the combination of CYCLOPS and autoactive calcium and calmodulin-dependent kinase (CCaMK) via GCC boxes. Knockdown of NF-YC3 significantly reduced the abundance of all intraradical fungal structures and affected arbuscule size. BCP1, SbtM1, and WRI5a, whose expression associated with NF-YC3 levels, might be downstream of NF-YC3. NF-YC3 interacted with NF-YB3a, NF-YB5c, or NF-YB3b, in yeast (Saccharomyces cerevisiae) and in planta, and interacted with NF-YA3a in yeast. Spatial expression of three NF-YBs was observed in all cell layers of roots under both mock and mycorrhizal conditions. Simultaneous knockdown of three NF-YBs, but not individually, reduced the fungal colonization level, suggesting that there might be functional redundancy of NF-YBs to regulate AM symbiosis. Collectively, our data suggest that NF-YC3 and NF-YBs positively regulate AM symbiosis in tomato, and arbuscule-related NF-YC3 may be an important downstream gene of the common symbiosis signaling pathway.

FRAX486, a PAK inhibitor, overcomes ABCB1-mediated multidrug resistance in breast cancer cells.

The overexpression of P-glycoprotein (P-gp/ABCB1) is a leading cause of multidrug resistance (MDR). Hence, it is crucial to discover effective pharmaceuticals that counteract ABCB1-mediated multidrug resistance. FRAX486 is a p21-activated kinase (PAK) inhibitor. The objective of this study was to investigate whether FRAX486 can reverse ABCB1-mediated multidrug resistance, while also exploring its mechanism of action. The CCK8 assay demonstrated that FRAX486 significantly reversed ABCB1-mediated multidrug resistance. Furthermore, western blotting and immunofluorescence experiments revealed that FRAX486 had no impact on expression level and intracellular localization of ABCB1. Notably, FRAX486 was found to enhance intracellular drug accumulation and reduce efflux, resulting in the reversal of multidrug resistance. Docking analysis also indicated a strong affinity between FRAX486 and ABCB1. This study highlights the ability of FRAX486 to reverse ABCB1-mediated multidrug resistance and provides valuable insights for its clinical application.

Metals at the Host-Fungal Pathogen Battleground.

Fungal infections continue to represent a major threat to public health, particularly with the emergence of multidrug-resistant fungal pathogens. As part of the innate immune response, the host modulates the availability of metals as armament against pathogenic microbes, including fungi. The transition metals Fe, Cu, Zn, and Mn are essential micronutrients for all life forms, but when present in excess, these same metals are potent toxins. The host exploits the double-edged sword of these metals, and will either withhold metal micronutrients from pathogenic fungi or attack them with toxic doses. In response to these attacks, fungal pathogens cleverly adapt by modulating metal transport, metal storage, and usage of metals as cofactors for enzymes. Here we review the current state of understanding on Fe, Cu, Zn, and Mn at the host-fungal pathogen battleground and provide perspectives for future research, including a hope for new antifungals based on metals.

Alginate-based adsorbents with adjustable slit-shaped pore structure for selective removal of copper ions.

Adopting effective and efficient techniques for the treatment of heavy metal pollution in water bodies plays an important role in guaranteeing the quality of water and the sustainable development of water resources. In this study, GO, MMT and SA were used as raw materials to compare the adsorption behaviors of three alginate-based adsorbents crosslinked with different valence metal ions (Ca2+, Fe3+ and Zr4+) on Cu(II). The aerogels were based on sodium alginate as the matrix material with unique slit-shaped pore structures formed by stacking effect of sheets and chemical bonding. It was found that the pore structures of the aerogels were denser and more orderly with the increase of the valence states of the crosslinked ions, and the affinity for Cu(II) in planar configuration was stronger. The Zr4+-GMSA aerogel had the maximum adsorption capacity of 126.68 mg/g and the Kd of Cu(II) was up to 50.80 L/g, which exhibited good preferential adsorption performance. The adsorption mechanism of Mn+-GMSA aerogels on Cu(II) was mainly ionic exchange, surface complexation and physical adsorption, which was explored by combining XPS and EDS characterizations of Mn+-GMSA before and after adsorption. This scheme can provide valuable and meaningful contribution to realize the selective recovery of Cu(II).

Transition-state defect structure: A new strategy for TiO2-based porous materials to enhance photodegradation of pollutants.

The aim of this paper is to investigate the derived structure and properties of Zeolitic Imidazolate Framework-8 (ZIF-8), and the effect of residual structural on the catalytic properties after loading with Titanium Dioxide (TiO2). For this purpose, we ingeniously prepare C-ZIF-8@TiO2 with a transition-state defect structure and apply it for efficiently degrading organic dye wastewater represented by Rhodamine B (Rh-B). Thanks to the transition-state defect structure loaded with TiO2 and ZIF-8 self-derived Carbon (C) and Zinc Oxide (ZnO), the catalytic performance of C-ZIF-8@TiO2 is superior to that of TiO2 and normal TiO2/ZIF-8 composites, and it is effective in degrading a variety of antibiotics and dyes. The related characterization also shows good photovoltaic properties and long-term durability for C-ZIF-8@TiO2. The mechanism on free radical action is elucidated and the possible degradation pathway for Rh-B is speculated. Therefore, C-ZIF-8@TiO2 provides a new strategy for the degradation of organic pollutants in water bodies.

Milestones in understanding transport, sensing, and signaling of the plant nutrient phosphorus.

As an essential nutrient element, phosphorus (P) is primarily acquired and translocated as inorganic phosphate (Pi) by plant roots. Pi is often sequestered in the soil and becomes limited for plant growth. Plants have developed a sophisticated array of adaptive responses, termed P starvation responses, to cope with P deficiency by improving its external acquisition and internal utilization. Over the past 2 to 3 decades, remarkable progress has been made toward understanding how plants sense and respond to changing environmental P. This review provides an overview of the molecular mechanisms that regulate or coordinate P starvation responses, emphasizing P transport, sensing, and signaling. We present the major players and regulators responsible for Pi uptake and translocation. We then introduce how P is perceived at the root tip, how systemic P signaling is operated, and the mechanisms by which the intracellular P status is sensed and conveyed. Additionally, the recent exciting findings about the influence of P on plant-microbe interactions are highlighted. Finally, the challenges and prospects concerning the interplay between P and other nutrients and strategies to enhance P utilization efficiency are discussed. Insights obtained from this knowledge may guide future research endeavors in sustainable agriculture.

Preparation of Mn modified waste dander biochar and its effect on soil carbon sequestration.

In order to reduce the mineralization of soil organic carbon (SOC) and enhance the ability of soil carbon sequestration. Mn-modified waste dander biochar (Mn-BC) was successfully prepared via impregnation and pyrolysis, and MnSO4 was formed on its surface. Mn-BC increases the carbon retention and reduces the emissions of CO2 and SO2 in way of forming CO, Mn-O-C bond and MnSO4. At the same time, the stability of the original biochar was reserved due to forming a conjugated structure (CC and pyridine-N bond), and the carbon sequestration content was increased to 25.63%. Importantly, the application of Mn-BC can directly regulate the transformation of microbial bacterial community and lead to create stable carbon dominant bacteria (Firmicutes). And the mineralization rate of SOC is reduced to 0.48 mg CO2/(g·d), together with an increased content of TOC (48.16%), thus the purpose of efficient carbon sequestration is achieved in soil.

NaH2PO4 synergizes with organic matter to stabilize chromium in tannery sludge.

Heavy metal stabilization is an effective method to treat chromium in tannery sludge. Here we show that mainly investigated NaH2PO4 (MSP) and organic matter (OM) to stabilize chromium in tannery sludge. The experimental investigation revealed that the addition of montmorillonite (MMT) and MSP samples showed a significant increase in the percentage of reducible and oxidizable Cr in the former compared to the samples with the addition of MMT. This is attributed to the formation of Cr-O bond, which allows the MSP to undergo an inner-sphere complexation reaction with the metal oxide of Cr via ligand exchange. Significantly, the MSP moiety adsorbs on the surface of OM through monodentate, which increases the adsorption sites of OM for Cr6+ and promotes the reduction of Cr6+ to Cr3+. Moreover, PO43- reacts with Cr3+ to produce CrPO4 precipitation, thus reducing the free Cr3+ content. Finally, DFT calculations confirmed that a ternary system is formed between PO43-, OM, and Cr, and the binding energy is negative, which indicated that PO43- could co-stabilize Cr with OM.

Design and oxidative desulfurization of Ag/Ti heterometallic clusters based on Hard-Soft Acid-Base principle.

Hard-Soft Acid-Base (HSAB) principle plays an important guiding role in the design and synthesis of novel clusters and coordination compounds, in which "soft acids prefer to react with soft bases, while hard acids have an affinity for hard bases". Based on HSAB principle, four Ag/Ti heterometallic clusters, including Ag2Ti10, Ag2Ti11 with "Ti-encapsulated Ag" configurations, and two "Ag-encapsulated Ti" structures Ag2Ti2 and Ag2Ti12, were synthesized under solvothermal conditions. In addition, Ag2Ti12 exhibited an efficient and stable catalytic activity for sulfide oxidation. This work provides not only a new structural model for the modulation of the catalytic oxidative desulfurization properties of Ag/Ti heterometallic clusters but also a new insight of the utilization of phosphine-containing ligands to regulate the structure of Ag/Ti heterometallic clusters.

Preparation and Density Functional Theory Studies of Aluminosilicate-Based Ceramic Solidified Products for Sr Immobilization.

Strontium is a common radionuclide in radioactive waste, and its release into the environment can cause enormous damage to the ecosystem environment. In this study, the natural mineral allophane was selected as the substrate to prepare solidified ceramic products by cold pressing/sintering to solve the problem of the final disposal of radioactive strontium. Ceramic solidified products with various crystal structures were successfully prepared, and the microscopic morphology and energy-dispersive spectroscopy images of the samples showed a uniform distribution of Sr in the solidified products. Sr2Al2SiO7 and SrAl2Si2O8, which can stably solidify strontium, were formed in the solidified products, and the structural characteristics and stability of the above-mentioned substances were analyzed from the perspective of quantum chemical calculations using density functional theory. The calculation results showed that the overall deformation resistance of Sr2Al2SiO7 was higher than that of SrAl2Si2O8. Considering the isomorphic substitution effect of CaO impurities, we inferred that a mixed-crystalline structure of Ca2-xSrxAl2SiO7 may be present in the solidified products.

Index of consciousness monitoring during general anesthesia may effectively enhance rehabilitation in elderly patients undergoing laparoscopic urological surgery: a randomized controlled clinical trial.

BACKGROUND: Based on electroencephalogram (EEG) analysis, index of consciousness (IoC) monitoring is a new technique for monitoring anesthesia depth. IoC is divided into IoC1 (depth of sedation) and IoC2 (depth of analgesia). The potential for concurrent monitoring of IoC1 and IoC2 to expedite postoperative convalescence remains to be elucidated. We investigated whether combined monitoring of IoC1 and IoC2 can effectively enhances postoperative recovery compared with bispectral index (BIS) in elderly patients undergoing laparoscopic urological surgery under general anesthesia. METHODS: In this prospective, controlled, double-blinded trail, 120 patients aged 65 years or older were arbitrarily assigned to either the IoC group or the control group (BIS monitoring). All patients underwent blood gas analysis at T1 (before anesthesia induction) and T2 (the end of operation). The Mini-Mental State Examination (MMSE) and Montreal Cognitive Assessment (MoCA) were administered to all patients at T0 (1 day before surgery) and T4 (7 days after surgery). Serum concentrations of C-reactive protein (CRP) and glial fibrillary acid protein (GFAP) were assessed at T1, T2, and T3 (24 h after surgery). Postoperative complications and the duration of hospitalization were subjected to comparative evaluation. RESULTS: The incidence of postoperative cognitive dysfunction (POCD) was notably lower in the IoC group (10%) than in the control group (31.7%) (P = 0.003). Postoperative serum CRP and GFAP concentrations exhibited significant differences at time points T2 (CRP: P = 0.000; GFAP: P = 0.000) and T3 (CRP: P = 0.003; GFAP: P = 0.008). Postoperative blood glucose levels (P = 0.000) and the overall rate of complications (P = 0.037) were significantly lower in Group IoC than in Group control. CONCLUSION: The employment of IoC monitoring for the management of elderly surgical patients can accelerate postoperative convalescence by mitigating intraoperative stress and reducing peripheral and central inflammatory injury. TRIAL REGISTRATION: Chinese Clinical Trial Registry Identifier: ChiCTR1900025241 (17/08/2019).

Photocatalytic Co-Reduction of N2 and CO2 with CeO2 Catalyst for Urea Synthesis.

The effective conversion of carbon dioxide (CO2 ) and nitrogen (N2 ) into urea by photocatalytic reaction under mild conditions is considered to be a more environmentally friendly and promising alternative strategies. However, the weak adsorption and activation ability of inert gas on photocatalysts has become the main challenge that hinder the advancement of this technique. Herein, we have successfully established mesoporous CeO2-x nanorods with adjustable oxygen vacancy concentration by heat treatment in Ar/H2 (90 % : 10 %) atmosphere, enhancing the targeted adsorption and activation of N2 and CO2 by introducing oxygen vacancies. Particularly, CeO2 -500 (CeO2 nanorods heated treatment at 500 °C) revealed high photocatalytic activity toward the C-N coupling reaction for urea synthesis with a remarkable urea yield rate of 15.5 µg/h. Besides, both aberration corrected transmission electron microscopy (AC-TEM) and Fourier transform infrared (FT-IR) spectroscopy were used to research the atomic surface structure of CeO2 -500 at high resolution and to monitor the key intermediate precursors generated. The reaction mechanism of photocatalytic C-N coupling was studied in detail by combining Density Functional Theory (DFT) with specific experiments. We hope this work provides important inspiration and guiding significance towards highly efficient photocatalytic synthesis of urea.

Optimization and validation of voxel size-related radiomics variability by combatting batch effect harmonization in pulmonary nodules: a phantom and clinical study.

Background: Broad generalization of radiomics-assisted models may be impeded by concerns about variability. This study aimed to evaluate the merit of combatting batch effect (ComBat) harmonization in reducing the variability of voxel size-related radiomics in both phantom and clinical study in comparison with image resampling correction method. Methods: A pulmonary phantom with 22 different types of nodules was scanned by computed tomography (CT) with different voxel sizes. The variability of voxel size-related radiomics features was evaluated using concordance correlation coefficient (CCC), dynamic range (DR), and intraclass correlation coefficient (ICC). ComBat and image resampling compensation methods were used to reduce variability of voxel size-related radiomics. The percentage of robust radiomics features was compared before and after optimization. Pathologically differential diagnosis of invasive adenocarcinoma (IAC) from adenocarcinoma in situ (AIS) and minimally invasive adenocarcinoma (MIA) (AIS-MIA group) was used for clinical validation in 134 patients. Results: Before optimization, the number of excellent features in the phantom and clinical data was 26.12% and 32.31%, respectively. The excellent features were increased after image resampling and ComBat correction. For clinical optimization, the effect of the ComBat compensation method was significantly better than that of image resampling, with excellent features reaching 90.96% and poor features only amounting to 4.96%. In addition, the hierarchical clustering analysis showed that the first-order and shape features had better robustness than did texture features. In clinical validation, the area under the curve (AUC) of the testing set was 0.865 after ComBat correction. Conclusions: The ComBat harmonization can optimize voxel size-related CT radiomics variability in pulmonary nodules more efficiently than image resampling harmonization.

Risk factors for postoperative acute ischemic stroke in advanced-aged patients with previous stroke undergoing noncardiac surgery: a retrospective cohort study.

BACKGROUND: The current study aimed to investigate the incidence and risk factors for postoperative acute ischemic stroke (PAIS) in advanced-aged patients (≥ 75 years) with previous ischemic stroke undergoing noncardiac surgery. METHODS: In this single-center retrospective cohort study, all advanced-aged patients underwent noncardiac surgery from 1 January, 2019, to 30 April, 2022. Data were extracted from hospital electronic medical records. Multivariable logistic regression analysis was performed to determine predictors of PAIS. Multivariable linear or logistic regression analysis was performed to determine predictors of outcomes due to PAIS. RESULTS: Twenty-four patients (6.0%) of the 400 patients developed PAIS. Carotid endarterectomy (CEA), length of surgery and preoperative Modified Rankin scale (mRS) ≥ 3 were significant predictors of PAIS. CEA was associated with increased risk of PAIS (OR 4.14; 95%CI, 1.43-11.99). Each additional minute in length of surgery had slightly increased the risk of PAIS (OR, 1.01; 95%CI, 1.00-1.01). Compared with reference (mRS < 3), mRS ≥ 3 increased odds of PAIS (OR, 4.09;95%CI, 1.12-14.93). Surgery type and length of surgery were found to be significant predictors of in-hospital expense (P < 0.001) and hospital stays (P < 0.05). CONCLUSIONS: CEA, length of surgery and preoperative mRS ≥ 3 may increase the development of PAIS in advanced-aged patients (≥ 75 years) with previous stroke undergoing noncardiac surgery. PAIS increased in-hospital mortality and prolonged hospital stay.

Stabilizing Cr(â ¢) deriving from tannery sludge with kaolin and organic matter.

Stabilizing Cr(III) in tannery sludge (TS) via harmless method has always been the goal of environmental pollution treatment. In this study, a simple method to stabilize Cr(III) in TS is proposed via adding kaolin, based on the fact a large amount of organic matter contained in TS. Comprehensive characterizations confirm that kaolin can stabilize Cr(Ⅲ) via its abundant -OH and lamellar structure. Moreover, there are hydrogen bond interactions and ligand exchange-surface complexation between organic matter and kaolin, which is more conducive to form a stable ternary complex with Cr(III), in a state of organic matter-Cr(III)-kaolin. Simultaneously, the BCR sequential extraction experiment shows that the unstable water and acid soluble state of Cr(III) are reduced (from 0.61% to 0.35%), which further indicates that the stabilization of Cr(III) is successful.

Pre-incisional Preventive Precise Multimodal Analgesia May Enhance the Rehabilitation Process of Acute Postoperative Pain Following Total Laparoscopic Hysterectomy: A Randomized Controlled Trial.

BACKGROUND: There has been limited research regarding the effect of preventive precise multimodal analgesia (PPMA) on the duration of acute postoperative pain after total laparoscopic hysterectomy (TLH). This randomized controlled trial aimed to evaluate how PPMA affects pain rehabilitation. OBJECTIVES: Our primary objective was to reduce the duration of acute postoperative pain after TLH, including incisional and visceral pain. STUDY DESIGN: A double blind randomized controlled clinical trial. SETTING: Department of Anesthesiology, Xuanwu Hospital, Capital Medical University, Beijing, People's Republic of China. METHODS: Seventy patients undergoing TLH were randomized to Group PPMA or Group Control (Group C) in a 1:1 ratio. Patients in Group PPMA were given PPMA through the pre-incisional administration of parecoxib sodium 40 mg (parecoxib is not approved for use in the US) and oxycodone 0.1 mg/kg as well as local anesthetic infiltration at the incision sites. In Group C, similar doses of parecoxib sodium and oxycodone were injected during uterine removal, and a local anesthetic infiltration procedure was performed immediately before skin closure. The index of consciousness 2 was utilized to titrate the remifentanil dosage in all patients to ensure sufficient analgesia. RESULTS: Compared with the Control, PPMA shortened the durations of incisional and visceral pain at rest (median, interquartile range [IQR]: 0, 0.0- 2.5) vs 2.0, 0.0-48.0 hours, P = 0.045; 24.0, 6.0-24.0 vs 48.0, 24.0-48.0 hours, P < 0.001; and during coughing 1.0, 0.0-3.0 vs 24.0, 0.3-48.0 hours, P = 0.001; 24.0, 24.0-48.0] vs 48.0, 48.0-72.0] hours, P < 0.001). The Visual Analog Scale (VAS) scores for incisional pain within 24 hours and visceral pain within 48 hours in Group PPMA were lower than those in Group C (P < 0.05). PPMA evidently decreased the VAS scores for incisional pain during coughing at 48 hours (P < 0.05). Pre-incisional PPMA significantly reduced postoperative opioid consumption (median, IQR: 3.0 [0.0-3.0] vs 3.0 [0.8-6.0] mg, P = 0.041) and the incidence of postoperative nausea and vomiting (25.0% vs 50.0%, P = 0.039). Postoperative recovery and hospital stay were similar between the 2 groups. LIMITATIONS: This research had some limitations, including that it was a single-center research with a limited sample size. Our study cohort did not represent the overall patient population in the People's Republic of China; therefore, the external validity of our findings remains limited. Furthermore, the prevalence of chronic pain was not tracked. CONCLUSION: Pre-incisional PPMA may enhance the rehabilitation process of acute postoperative pain after TLH.

In situ formed CaSO4 on waste dander biochar to inhibit the mineralization of soil organic carbon.

In order to reduce CO2 emissions, as well as realize the resource utilization of waste dander (WD) and the goal of international "peak carbon dioxide emissions" and "carbon neutrality", Biochar was prepared with WD via pyrolysis technology, achieving CaSO4 in situ generated on its surface, which could be used to inhibit soil organic carbon (SOC) from mineralizing and enhance soil carbon sequestration ability. The characterization results showed that the unstable carbon (C) structures as well as more conjugated structures were generated on Ca-BC, obtaining an increased C sequestration of Ca-BC to 21.70 %. With the application of Ca-BC, the mineralization rate of SOC was reduced to 0.451 mg CO2/(g·d), and the soil moisture content, pH and TOC content were increased to 45.48 %, 7.96 and 47.19 %. In addition, the bioinformatics analysis and redundancy analysis revealed that the application of Ca-BC promoted bacteria to convert into the stable C-dominant phyla (Firmicutes).

Resting-state functional connectivity alteration in elderly patients with knee osteoarthritis and declined cognition: An observational study.

Objective: This study is designed to investigate the brain function changed regions in elderly patients with knee osteoarthritis (KOA) and to explore the relationship between neuropsychological tests and resting-state functional magnetic resonance imaging (rs-fMRI) network to clarify the possible mechanism underlying cognitive changes in KOA patients. Materials and methods: Fifty-two patients aged ≥ 65 with KOA and twenty-two healthy-matched controls were recruited in this study. All participants were given rs-fMRI check. We used graph theory analysis to characterize functional connectivity (FC) and topological organization of the brain structural network. The relationship between FC values, topological properties, and the neuropsychological test scores was analyzed. Results: Compared with the controls, fourteen edges with lower functional connectivity were noted in the KOA group. Local efficiency and small-worldness of KOA patients decreased compared to the healthy controls. No significant alterations of nodal topological properties were found between the two groups. There was a significant positive correlation between the AVLT-H (L) and the internetwork of default mode network (DMN) (left/right orbitofrontal Superior cortex) and limbic/cortical areas (left/right caudate, right amygdala). AVLT-H(L) was positively correlated with small-worldness and local efficiency. Conclusion: The results indicated that for elderly KOA patients with declined cognition, topological properties, FC between DMN and subcortical limbic network related regions are significantly decreased compared to healthy controls. These alterations demonstrated a significant correlation with the neuropsychological test scores.

Sensitivity and specificity of magnetic resonance imaging in routine diagnosis of pulmonary lesions: a comparison with computed tomography.

Background: State-of-the-art thoracic magnetic resonance imaging (MRI) plays a complementary role in the assessment of pulmonary nodules/masses which potentially indicate to cancer. We aimed to evaluate the sensitivity and specificity of MRI in diagnosis of pulmonary nodules/masses. Methods: Sixty-eight patients with computed tomography (CT)-detected pulmonary nodules/masses underwent 3T MRI (T1-VIBE, T1-starVIBE, T2-fBLADE turbo spin-echo, and T2-SPACE). The detection rate was calculated for each of the different subgroups of pulmonary nodules according to lung imaging reporting and data system (Lung-RADS). The four MRI sequences were compared in terms of detection rate and image quality-signal to noise ratio (SNR), contrast to noise ratio (CNR) and 5-point scoring scale. Agreement of lesion size measurement between CT and MRI was assessed by intraclass correlation coefficient (ICC). The picture-SNR, lesion-SNR and CNR of each sequence were analyzed by Mann-Whitney U test. Results: In total, 232 pulmonary lesions were detected by CT. The CT showed 86 solid nodules (SNs) <6 mm, 15 SNs between 6-8 mm, 35 SNs between 8-15 mm, and 52 SNs between 15-30 mm. The T1-VIBE, T1-starVIBE, T2-fBLADE TSE and T2-SPACE sequences accurately detected 141 SNs (141/188, 75%/83.3%), 150 SNs (150/188, 79.8%/100%), 166 SNs (166/188, 88.3%/66.7%) and 169 SNs (169/188, 89.9%/53.3%), respectively. Four ground glass nodules (GGNs) (4/6) were detected by T2-fBLADE TSE. Twelve part-solid nodules (PSNs) (12/22) were detected by T1-VIBE and 20 PSNs (20/22) by T2-SPACE. A total of 100 lesions (2.2±1.4 cm, 0.8-7.3 cm) were accurately detected and measured by the four MRI sequences with ICC >0.96. The picture-SNR, lesion-SNR and CNR by T1-starVIBE were higher than those by T1-VIBE (P<0.001). The lesion-SNR and CNR by T2-fBLADE TSE were higher than those by T2-SPACE (P=0.006, 0.038). 86% of images by T1-starVIBE, 92% by T2-fBLADE TSE, 90% by T2-SPACE and 93% by T1-VIBE were scored 3 or more. Conclusions: MRI achieves high sensitivity and specificity for different type of pulmonary nodules detection and is an effective alternative to CT as a diagnostic tool for pulmonary nodules.