Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 11 de 11
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Mol Plant ; 17(4): 509-512, 2024 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-38327053

RESUMO

As the master regulators of the ET signaling pathway, EIL transcription factors directly activate the expression of CYP94C1 to inactivate bioactive JA-Ile, thereby attenuating JA-mediated defense during fruit ripening. Knockout of CYP94C1 improves tomato fruit resistance to necrotrophs without compromising fruit quality.


Assuntos
Isoleucina/análogos & derivados , Solanum lycopersicum , Solanum lycopersicum/genética , Frutas/genética , Frutas/metabolismo , Oxilipinas/metabolismo , Ciclopentanos/metabolismo , Regulação da Expressão Gênica de Plantas
2.
Plant Cell Rep ; 43(3): 75, 2024 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-38381195

RESUMO

KEY MESSAGE: Single-cell transcriptomic techniques have emerged as powerful tools in plant biology, offering high-resolution insights into gene expression at the individual cell level. This review highlights the rapid expansion of single-cell technologies in plants, their potential in understanding plant development, and their role in advancing plant biotechnology research. Single-cell techniques have emerged as powerful tools to enhance our understanding of biological systems, providing high-resolution transcriptomic analysis at the single-cell level. In plant biology, the adoption of single-cell transcriptomics has seen rapid expansion of available technologies and applications. This review article focuses on the latest advancements in the field of single-cell transcriptomic in plants and discusses the potential role of these approaches in plant development and expediting plant biotechnology research in the near future. Furthermore, inherent challenges and limitations of single-cell technology are critically examined to overcome them and enhance our knowledge and understanding.


Assuntos
Biotecnologia , Transcriptoma , Transcriptoma/genética , Perfilação da Expressão Gênica , Desenvolvimento Vegetal
4.
Nat Plants ; 9(10): 1659-1674, 2023 10.
Artigo em Inglês | MEDLINE | ID: mdl-37723204

RESUMO

Crop breeding for mechanized harvesting has driven modern agriculture. In tomato, machine harvesting for industrial processing varieties became the norm in the 1970s. However, fresh-market varieties whose fruits are suitable for mechanical harvesting are difficult to breed because of associated reduction in flavour and nutritional qualities. Here we report the cloning and functional characterization of fs8.1, which controls the elongated fruit shape and crush resistance of machine-harvestable processing tomatoes. FS8.1 encodes a non-canonical GT-2 factor that activates the expression of cell-cycle inhibitor genes through the formation of a transcriptional module with the canonical GT-2 factor SlGT-16. The fs8.1 mutation results in a lower inhibitory effect on the cell proliferation of the ovary wall, leading to elongated fruits with enhanced compression resistance. Our study provides a potential route for introducing the beneficial allele into fresh-market tomatoes without reducing quality, thereby facilitating mechanical harvesting.


Assuntos
Solanum lycopersicum , Solanum lycopersicum/genética , Frutas/genética , Frutas/metabolismo , Melhoramento Vegetal , Agricultura
5.
Hortic Res ; 10(1): uhac214, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36643741

RESUMO

Fruit color is an important horticultural trait, which greatly affects consumer preferences. In tomato, fruit color is determined by the accumulation of different pigments, such as carotenoids in the pericarp and flavonoids in the peel, along with the degradation of chlorophyll during fruit ripening. Since fruit color is a multigenic trait, it takes years to introgress all color-related genes in a single genetic background via traditional crossbreeding, and the avoidance of linkage drag during this process is difficult. Here, we proposed a rapid breeding strategy to generate tomato lines with different colored fruits from red-fruited materials by CRISPR/Cas9-mediated multiplex gene editing of three fruit color-related genes (PSY1, MYB12, and SGR1). Using this strategy, the red-fruited cultivar 'Ailsa Craig' has been engineered to a series of tomato genotypes with different fruit colors, including yellow, brown, pink, light-yellow, pink-brown, yellow-green, and light green. Compared with traditional crossbreeding, this strategy requires less time and can obtain transgene-free plants with different colored fruits in less than 1 year. Most importantly, it does not alter other important agronomic traits, like yield and fruit quality. Our strategy has great practical potential for tomato breeding and serves as a reference for improving multigene-controlled traits of horticultural crops.

6.
Plant Cell ; 35(3): 1038-1057, 2023 03 15.
Artigo em Inglês | MEDLINE | ID: mdl-36471914

RESUMO

Fruit ripening relies on the precise spatiotemporal control of RNA polymerase II (Pol II)-dependent gene transcription, and the evolutionarily conserved Mediator (MED) coactivator complex plays an essential role in this process. In tomato (Solanum lycopersicum), a model climacteric fruit, ripening is tightly coordinated by ethylene and several key transcription factors. However, the mechanism underlying the transmission of context-specific regulatory signals from these ripening-related transcription factors to the Pol II transcription machinery remains unknown. Here, we report the mechanistic function of MED25, a subunit of the plant Mediator transcriptional coactivator complex, in controlling the ethylene-mediated transcriptional program during fruit ripening. Multiple lines of evidence indicate that MED25 physically interacts with the master transcription factors of the ETHYLENE-INSENSITIVE 3 (EIN3)/EIN3-LIKE (EIL) family, thereby playing an essential role in pre-initiation complex formation during ethylene-induced gene transcription. We also show that MED25 forms a transcriptional module with EIL1 to regulate the expression of ripening-related regulatory as well as structural genes through promoter binding. Furthermore, the EIL1-MED25 module orchestrates both positive and negative feedback transcriptional circuits, along with its downstream regulators, to fine-tune ethylene homeostasis during fruit ripening.


Assuntos
Solanum lycopersicum , Fatores de Transcrição , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Solanum lycopersicum/genética , Frutas/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Etilenos/metabolismo , Regulação da Expressão Gênica de Plantas
8.
Plant Cell ; 29(9): 2249-2268, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28814642

RESUMO

Deciphering the mechanism of malate accumulation in plants would contribute to a greater understanding of plant chemistry, which has implications for improving flavor quality in crop species and enhancing human health benefits. However, the regulation of malate metabolism is poorly understood in crops such as tomato (Solanum lycopersicum). Here, we integrated a metabolite-based genome-wide association study with linkage mapping and gene functional studies to characterize the genetics of malate accumulation in a global collection of tomato accessions with broad genetic diversity. We report that TFM6 (tomato fruit malate 6), which corresponds to Al-ACTIVATED MALATE TRANSPORTER9 (Sl-ALMT9 in tomato), is the major quantitative trait locus responsible for variation in fruit malate accumulation among tomato genotypes. A 3-bp indel in the promoter region of Sl-ALMT9 was linked to high fruit malate content. Further analysis indicated that this indel disrupts a W-box binding site in the Sl-ALMT9 promoter, which prevents binding of the WRKY transcription repressor Sl-WRKY42, thereby alleviating the repression of Sl-ALMT9 expression and promoting high fruit malate accumulation. Evolutionary analysis revealed that this highly expressed Sl-ALMT9 allele was selected for during tomato domestication. Furthermore, vacuole membrane-localized Sl-ALMT9 increases in abundance following Al treatment, thereby elevating malate transport and enhancing Al resistance.


Assuntos
Alumínio/toxicidade , Domesticação , Frutas/metabolismo , Mutação INDEL/genética , Malatos/metabolismo , Proteínas de Plantas/genética , Regiões Promotoras Genéticas/genética , Solanum lycopersicum/genética , Adaptação Fisiológica/efeitos dos fármacos , Adaptação Fisiológica/genética , Sequência de Bases , Sistemas CRISPR-Cas/genética , Mapeamento Cromossômico , Segregação de Cromossomos , Frutas/genética , Regulação da Expressão Gênica de Plantas , Marcadores Genéticos , Variação Genética , Estudo de Associação Genômica Ampla , Técnicas de Genotipagem , Humanos , Solanum lycopersicum/efeitos dos fármacos , Proteínas de Plantas/metabolismo , Deleção de Sequência , Estresse Fisiológico/genética , Fatores de Transcrição/metabolismo
9.
Plant Cell ; 29(8): 1883-1906, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28733419

RESUMO

The hormone jasmonate (JA), which functions in plant immunity, regulates resistance to pathogen infection and insect attack through triggering genome-wide transcriptional reprogramming in plants. We show that the basic helix-loop-helix transcription factor (TF) MYC2 in tomato (Solanum lycopersicum) acts downstream of the JA receptor to orchestrate JA-mediated activation of both the wounding and pathogen responses. Using chromatin immunoprecipitation sequencing (ChIP-seq) coupled with RNA sequencing (RNA-seq) assays, we identified 655 MYC2-targeted JA-responsive genes. These genes are highly enriched in Gene Ontology categories related to TFs and the early response to JA, indicating that MYC2 functions at a high hierarchical level to regulate JA-mediated gene transcription. We also identified a group of MYC2-targeted TFs (MTFs) that may directly regulate the JA-induced transcription of late defense genes. Our findings suggest that MYC2 and its downstream MTFs form a hierarchical transcriptional cascade during JA-mediated plant immunity that initiates and amplifies transcriptional output. As proof of concept, we showed that during plant resistance to the necrotrophic pathogen Botrytis cinerea, MYC2 and the MTF JA2-Like form a transcription module that preferentially regulates wounding-responsive genes, whereas MYC2 and the MTF ETHYLENE RESPONSE FACTOR.C3 form a transcription module that preferentially regulates pathogen-responsive genes.


Assuntos
Ciclopentanos/farmacologia , Oxilipinas/farmacologia , Imunidade Vegetal/efeitos dos fármacos , Proteínas de Plantas/metabolismo , Solanum lycopersicum/genética , Solanum lycopersicum/imunologia , Transcrição Gênica/efeitos dos fármacos , Motivos de Aminoácidos , Sítios de Ligação , Botrytis/fisiologia , Resistência à Doença/efeitos dos fármacos , Resistência à Doença/genética , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Ontologia Genética , Genes de Plantas , Solanum lycopersicum/efeitos dos fármacos , Modelos Biológicos , Doenças das Plantas/microbiologia , Imunidade Vegetal/genética , Regiões Promotoras Genéticas/genética , Ligação Proteica/efeitos dos fármacos , Análise de Sequência de RNA , Transcriptoma/genética
10.
Oncotarget ; 8(1): 1788-1795, 2017 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-27705946

RESUMO

OBJECTIVES: To compare the proportion of stage I lung cancer and population mortality in China to those in U.S. and Europe where lung cancer screening by low-dose computed tomography (LDCT) has been already well practiced. METHODS: The proportions of stage I lung cancer in LDCT screening population in U.S. and Europe were retrieved from NLST and NELSON trials. The general proportion of stage I lung cancer in China was retrieved from a rapid meta-analysis, based on a literature search in the China National Knowledge Infrastructure database. The lung cancer mortality and prevalence of China, U.S. and Europe was retrieved from Globocan 2012 fact sheet. Mortality-to-prevalence ratio (MPR) was applied to compare the population survival outcome of lung cancer. RESULTS: The estimated proportion of stage I lung cancer in China is merely 20.8% among hospital-based cross-sectional population, with relative ratios (RRs) being 2.40 (95% CI 2.18-2.65) and 2.98 (95% CI 2.62-3.38) compared by LDCT-screening population in U.S. and Europe trials, respectively. MPR of lung cancer is as high as 58.9% in China, with RRs being 0.46 (95% CI 0.31-0.67) and 0.58 (95% CI 0.39-0.85) compared by U.S. and Europe, respectively. CONCLUSIONS: By the epidemiological inference, the LDCT mass screening might be associated with increasing stage I lung cancer and therefore improving population survival outcome. How to translate the experiences of lung cancer screening by LDCT from developed counties to China in a cost-effective manner needs to be further investigated.


Assuntos
Detecção Precoce de Câncer/métodos , Neoplasias Pulmonares/diagnóstico por imagem , Neoplasias Pulmonares/epidemiologia , Tomografia Computadorizada por Raios X/métodos , Carcinoma Pulmonar de Células não Pequenas/diagnóstico , Carcinoma Pulmonar de Células não Pequenas/diagnóstico por imagem , Carcinoma Pulmonar de Células não Pequenas/mortalidade , China/epidemiologia , Europa (Continente)/epidemiologia , Humanos , Neoplasias Pulmonares/diagnóstico , Neoplasias Pulmonares/mortalidade , Programas de Rastreamento/métodos , Estadiamento de Neoplasias , Prognóstico , Carcinoma de Pequenas Células do Pulmão/diagnóstico , Carcinoma de Pequenas Células do Pulmão/diagnóstico por imagem , Carcinoma de Pequenas Células do Pulmão/mortalidade , Estados Unidos/epidemiologia
11.
Clin Nucl Med ; 38(3): 219-20, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23412599

RESUMO

Earlier studies indicate that abnormal liver and spleen uptake on bone scan has been observed in association with intravenous injection of Gd-DTPA contrast for MRI. All of these patients, however, received Gd-DTPA injection prior to (99m)Tc MDP administration. We report herein 3 patients with hepatic and splenic uptake on bone scan; all were administered (99m)Tc MDP before Gd-DTPA. Thus, we can conclude that Gd-DTPA after (99m)Tc MDP administration may also induce increased hepatic and splenic radioactivity on bone scan.


Assuntos
Osso e Ossos/diagnóstico por imagem , Meios de Contraste , Gadolínio , Fígado/metabolismo , Baço/metabolismo , Medronato de Tecnécio Tc 99m/administração & dosagem , Medronato de Tecnécio Tc 99m/metabolismo , Administração Intravenosa , Idoso , Transporte Biológico , Humanos , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Cintilografia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...