RESUMO
Objective: To analyze the intervention effects of targeted nursing based on goal management theory on pregnancy outcomes, blood pressure, postpartum self-efficacy, and quality of life in patients with preeclampsia. Methods: We retrospectively analyzed 90 cases of preeclampsia admitted to Huzhou Maternity & Child Health Care Hospital from January 2022 to June 2023. All patients met the complete inclusion criteria. They were divided into 2 groups based on different nursing interventions: the control group (n = 45) received routine nursing interventions, and the observation group (n = 45) received targeted nursing based on goal management theory. Pregnancy outcomes, blood pressure, postpartum self-efficacy, and quality of life were compared between the 2 groups. Results: The incidence of adverse pregnancy outcomes was 28.89% in the control group and was significantly lower in the observation group at 11.11% (P < .001). Before intervention, there were no significant differences in systolic blood pressure and diastolic blood pressure between the 2 groups (P > .05). After intervention, the systolic blood pressure and diastolic blood pressure were significantly lower in the observation group than in the control group (P < .001). Before intervention, there was no significant difference in Breastfeeding Self-Efficacy Scale scores between the 2 groups (P > .05). After intervention, the Breastfeeding Self-Efficacy Scale scores were significantly higher in the observation group than in the control group (P < .001). Before intervention, there was no significant difference in the Short Form 36 Health Survey scores between the 2 groups (P > .05). After intervention, the Short Form 36 Health Survey scores were significantly higher in the observation group than in the control group (P < .001). Conclusion: Compared with routine nursing, targeted nursing based on goal management theory had superior intervention effects on preeclampsia. It can further alleviate patients' blood pressure, promote postpartum self-efficacy, improve quality of life, and reduce the risk of adverse pregnancy outcomes. It is worthy of clinical application and promotion.
RESUMO
Histone deacetylase 2 (HDAC2) has been demonstrated to regulate trophoblast behaviors. However, its role in trophoblast pyroptosis remains unknown. This study sought to analyze the molecular mechanism of HDAC2 in trophoblast pyroptosis in PE. Expression levels of HDAC2, forkhead box O3 (FOXO3), and protein kinase R-like endoplasmic reticulum kinase (PERK) in placenta tissues and HTR8/SVneo cells and H3K27ac levels in cells were determined. Levels of IL-1ß and IL-18 in placenta tissues were determined, and their correlation with HDAC2 was analyzed. Cell proliferation, migration, and invasion were evaluated, and levels of pyroptosis-associated proteins and cytokines were determined. The enrichments of H3K27 acetylation (H3K27ac) and FOXO3 in the FOXO3/PERK promoter region were determined. HDAC2 was downregulated, and FOXO3, PERK, IL-1ß, and IL-18 levels were elevated in PE placenta tissues. In HTR8/SVneo cells, HDAC2 downregulation suppressed cell proliferation, migration, and invasion and increased pyroptosis. HDAC2 erased H3K27ac in the FOXO3 promoter region and repressed FOXO3, and FOXO3 bound to the PERK promoter and increased PERK transcription. Functional rescue experiments revealed that silencing FOXO3 or PERK counteracted HDAC2 downregulation-induced cell pyroptosis. Overall, HDAC2 downregulation enhanced H3K27ac to activate FOXO3 and PERK, leading to the occurrence of trophoblast pyroptosis in PE.
