[Genetic characterization analysis on the first imported measles virus of genotype D8 in Chinese mainland].

This study analyzed the genetic characterization on first imported measles virus of genotype D8 in Chinese mainland. Serums were collected from the suspicious MV patients to detect IgM antibody in ELISA. Throat swabs were cultured in Vero/SLAM cell line to get measles virus isolates. Part of the nucleotide sequence of the 3' terminus of nucleoprotein (N) gene of these isolates were amplified by RT-PCR, and the amplicons were directly sequenced. The phylogenetic analysis was based on the nucleotide sequence about 456 base pairs of the 3' terminus of nucleoprotein (N) gene. Results showed that it reported 1 105 suspicious measles cases in shanghai, 2012, including 590 confirmed cases and 2 clinical case. The reported morbidity was 2.52 per one hundred thousand. 247 measles viruses were isolated from 984 throat swabs specimen. Most of them belonged to sub-genotype H1a except Shanghai12-239 was genotype D8. The homology of nucleotide and amino acid sequences were 97.8% and 98.6% respectively between Shanghai12-239 and WHO reference strain (Manchester. UNK30.94(D8)AF280803). Those were 89.6%-94.5% and 88.7%-95.3% between Shanghai12-239 and WHO reference strains of other genotypes.

[Study on the quality of life among floating population in a community of Shanghai].

OBJECTIVE: To discover the current condition of the floating population's quality of life (QOL) in a community of Shanghai and find out the possible influencing factors. METHODS: 480 people identified as 'floating', were chosen by systematic sampling method, whose QOL was evaluated by WHOQOL-BREF (Chinese Version). A multiple linear regression model was used to analyze the relationship between the factors and the scores of the four domains. RESULTS: The physical and psychological scores of the floating population were 14.09 +/- 2.09 and 11.28 +/- 2.18 which were lower than the average levels of the Chinese population which were 15.8 +/- 2.9 and 14.3 +/- 2.5. Women showed higher score in the social relationship domain than men (P = 0.017). With the increase of age, the scores of physical and psychological domain were descending (P(Phys.) = 0.030, P(Phych.) = 0.023). The score in every domain was rising when the appetite was better or the family conflicts had a lighter impact on life (P < 0.01). CONCLUSION: The QOL of the young females was better than other groups. Considering the whole QOL of the floating population, their physical and psychological demands should be highly concerned.