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1.
Front Plant Sci ; 15: 1392924, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39006959

RESUMO

Roads are an increasingly prevalent form of human activity that drives the decrease in plant community functions and threatens global biodiversity. However, few studies have focused on the changes in the function and diversity of alpine meadows caused by road infrastructure in the Tibetan Plateau. In this study, the changes in species diversity, functional diversity, and community stability were examined at different distances from the Qinghai-Tibet highway. The results showed that the road intensified the degradation of vegetation, which significantly altered species diversity and community structure. This effect gradually decreased from near to far from the highway. Plant community cover and species diversity were highest at intermediate distances (50-100 m) from the roadway; species diversity and stability were lowest in the grassland most disturbed by the road (0 m), and species diversity and functional diversity tended to stabilize farther away from the road (250 m). Our findings indicate that changes in species diversity are synchronized with changes in functional diversity, which largely determines the outcome of degraded grassland community diversity and stability. Our results provide a reference point for restoring degraded alpine areas and mitigating the ecological impacts of roads.

2.
Artigo em Inglês | MEDLINE | ID: mdl-34948747

RESUMO

In recent years, highway construction in the Qinghai-Tibet Plateau (QTP) has developed rapidly. When the highway passes through grassland, the soil, vegetation, and ecological environment along the line are disturbed. However, the impact on soil bacteria is still unclear. Soil bacteria play an important role in the ecological environment. The Qinghai-Tibet Highway (QTH) was selected as the research object to explore the changes in bacterial community structure, vegetation, soil, and other indicators. The results showed that the highway-related activities increased the degradation of vegetation along the road, significantly changed the physical and chemical properties of soil, and caused heavy metal pollution. These environmental factors affected the diversity and community structure of soil bacteria. This kind of disturbance shows a trend of gradually increasing from near to far from the highway. Gemmatimonas, Terrimonas, Nitrospira and Bacillus are more tolerant to environmental changes along the highway, while Barnesiella, and Blastococcus are more sensitive. The content of nitrate decreased and the content of ammonium nitrogen increased in the disturbed area, increasing the abundance of nitrifying bacteria. Therefore, the main factor of the disturbance of the QTH on the grassland is the decline of soil nutrient content, and the supplement of soil nutrients such as carbon and nitrogen should be taken into account in the process of ecological restoration of grassland along the line.


Assuntos
Carbono , Solo , Bactérias , Carbono/análise , Pradaria , Nitrogênio/análise , Microbiologia do Solo , Tibet
3.
Appl Opt ; 59(17): E9-E16, 2020 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-32543507

RESUMO

Infrared spectrum analysis technology can perform fast and nondestructive detection of gas and has been widely used in many fields. This work studies the quantitative analysis technology of the infrared spectrum based on deep learning. The experimental results show that the quantitative analysis model of logging gas established here can reach 100% recognition accuracy for elemental gas; further, the accuracy rate of spectral of mixed gas recognition reached 98%, indicating that the infrared spectrum logging gas detection model based on deep learning can quickly and accurately perform quantitative analysis of logging gas.

4.
Sensors (Basel) ; 19(2)2019 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-30669310

RESUMO

A new solution to the high-quality 3D reverse modeling problem of complex surfaces for fine workpieces is presented using a laser line-scanning sensor. Due to registration errors, measurement errors, deformations, etc., a fast and accurate method is important in machine vision measurement. This paper builds a convenient and economic multi-view stereo (MVS) measurement system based on a linear stage and a rotary stage to reconstruct the measured object surface completely and accurately. In the proposed technique, the linear stage is used to generate the trigger signal and synchronize the laser sensor scanning; the rotary stage is used to rotate the object and obtain multi-view point cloud data, and then the multi-view point cloud data are registered and integrated into a 3D model. The measurement results show a measurement accuracy of 0.075 mm for a 360° reconstruction in 34 s, and some evaluation experiments were carried out to demonstrate the validity and practicability of the proposed technique.

5.
Sensors (Basel) ; 18(11)2018 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-30384497

RESUMO

This paper proposes a deep convolutional neural network (CNN) -based technique for the detection of micro defects on metal screw surfaces. The defects we consider include surface damage, surface dirt, and stripped screws. Images of metal screws with different types of defects are collected using industrial cameras, which are then employed to train the designed deep CNN. To enable efficient detection, we first locate screw surfaces in the pictures captured by the cameras, so that the images of screw surfaces can be extracted, which are then input to the CNN-based defect detector. Experiment results show that the proposed technique can achieve a detection accuracy of 98%; the average detection time per picture is 1.2 s. Comparisons with traditional machine vision techniques, e.g., template matching-based techniques, demonstrate the superiority of the proposed deep CNN-based one.

6.
Sensors (Basel) ; 18(9)2018 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-30200665

RESUMO

The non-contact three-dimensional measurement and reconstruction techniques have played a significant role in the packaging and transportation of precious cultural relics. This paper develops a structured light based three-dimensional measurement system, with a low-cost for cultural relics packaging. The structured light based system performs rapid measurements and generates 3D point cloud data, which is then denoised, registered and merged to achieve accurate 3D reconstruction for cultural relics. The multi-frequency heterodyne method and the method in this paper are compared. It is shown that the relative accuracy of the proposed low-cost system can reach a level of 1/1000. The high efficiency of the system is demonstrated through experimental results.

7.
Anal Chem ; 86(9): 4416-22, 2014 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-24708044

RESUMO

The oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) has great utility in bioanalysis such as peroxidase/peroxidase mimetic-based biosensing. In this paper, the behaviors of TMB oxidation intermediates/products in liquid/liquid biphasic systems have been investigated for the first time. The free radical, charge transfer complex, and diimine species generated by TMB oxidation are all positively charged under acidic and near-neutral conditions. Electron paramagnetic resonance and visible absorbance spectroscopy data demonstrate that these cationic species can be effectively transferred from an aqueous phase into a water-immiscible liquid phase functionalized by an appropriate cation exchanger. Accordingly, sensitive potential responses of TMB oxidation have been obtained on a cation exchanger-doped polymeric liquid membrane electrode under mildly acidic and near-neutral conditions. By using the membrane electrode responsive to TMB oxidations, two sensitive potentiometric biosensing schemes including the peroxidase-labeled sandwich immunoassay and G-quadruplex DNAzyme-based DNA hybridization assay have been developed. The obtained detection limits for the target antigen and DNA are 0.02 ng/mL and 0.1 nM, respectively. Coupled with other advantages such as low cost, high reliability, and ease of miniaturization and integration, the proposed polymeric liquid membrane electrode holds great promise as a facile and efficient transducer for TMB oxidation and related biosensing applications.


Assuntos
Benzidinas/metabolismo , Técnicas Biossensoriais , Eletrodos , Peroxidases/metabolismo , Polímeros/química , Potenciometria , Membranas Artificiais , Oxirredução
8.
Langmuir ; 26(18): 14879-84, 2010 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-20795663

RESUMO

Ordered two-dimensional (2D) arrays of ß-HgS nanocrystal aggregates were prepared successfully at the air/water interface through the interfacial reaction between Hg(2+) ions in the subphase and H(2)S in the gaseous phase under the direction of liquid-expanded monolayers of arachidic acid (AA). These 2D arrays are composed of hexagonal or quasi-hexagonal aggregates with the size of several hundreds of nanometers that consist of several tens of HgS nanocrystals with the size of several nanometers. The formed HgS nanocrystals together with AA molecules self-assembled into round aggregates due to the interactions between the species, and the aggregates self-assembled into 2D arrays further due to the attractions between them. During the self-assembly process, the soft round aggregates transformed into hexagonal or quasi-hexagonal ones. The experimental conditions, especially the phase states of the AA monolayers and temperature, have great influences on the formation of the 2D arrays. To the best of our knowledge, this is the first case to get 2D ordered arrays at the air/water interface through a one-step synthesis and assembly process.

9.
Ying Yong Sheng Tai Xue Bao ; 20(6): 1271-7, 2009 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-19795632

RESUMO

The stem radius growth (SRG) of six samples of Picea meyeri and five samples of Larix principis-rupprechtii nearby the tree-line of Luya Mountain in North China was measured by the dendrometer in the summer phase from July 15 to August 7 and the autumn phase from September 5 to October 9, 2007, with the environmental factors measured simultaneously. In the summer phase, there was no significant difference in the sensitivity of SRG to the environmental factors fluctuation between the two species; but in the relatively cold and dry autumn phase, the SRG of P. meyeri was more sensitive to the fluctuation of environmental factors. The accumulated SRG of the two species increased linearly in summer phase, but decreased first and kept stable then in autumn phase, with the growth increment of P. meyeri fluctuated more than that of L. principis-rupprechtii. The SRG of the two species correlated positively with hydrological factors and negatively with thermal factors, and the SRG of P. meyeri was strongly affected by air humidity and temperature, while that of L. principis-rupprechtii was mainly affected by soil moisture.


Assuntos
Ecossistema , Larix/crescimento & desenvolvimento , Picea/crescimento & desenvolvimento , Caules de Planta/crescimento & desenvolvimento , Altitude , China , Umidade , Larix/classificação , Picea/classificação , Solo/análise , Temperatura , Água/análise
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