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1.
PLoS One ; 13(1): e0191226, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29338047

RESUMO

The influenza virus RNA genome is transcribed and replicated in the context of the viral ribonucleoprotein (vRNP) complex by the viral RNA polymerase. The nucleoprotein (NP) is the structural component of the vRNP providing a scaffold for the viral RNA. In the vRNP as well as during transcription and replication the viral polymerase interacts with NP but it is unclear which parts of the polymerase and NP mediate these interactions. Previously the C-terminal '627' domain (amino acids 538-693) of PB2 was shown to interact with NP. Here we report that a fragment encompassing amino acids 146-185 of NP is sufficient to mediate this interaction. Using NMR chemical shift perturbation assays we show that amino acid region 601 to 607 of the PB2 '627' domain interacts with this fragment of NP. Substitutions of these PB2 amino acids resulted in diminished RNP activity and surface plasmon resonance assays showed that amino acids D605 was essential for the interaction with NP and V606 may also play a partial role in the interaction. Collectively these results reveal a possible interaction surface between NP and the PB2 subunit of the RNA polymerase complex.


Assuntos
Virus da Influenza A Subtipo H5N1/química , Virus da Influenza A Subtipo H5N1/genética , Proteínas de Ligação a RNA/química , Proteínas de Ligação a RNA/genética , RNA Polimerase Dependente de RNA/química , RNA Polimerase Dependente de RNA/genética , Proteínas do Core Viral/química , Proteínas do Core Viral/genética , Proteínas Virais/química , Proteínas Virais/genética , Substituição de Aminoácidos , Ácido Aspártico/química , Genoma Viral , Células HEK293 , Humanos , Virus da Influenza A Subtipo H5N1/fisiologia , Influenza Humana/virologia , Modelos Moleculares , Ressonância Magnética Nuclear Biomolecular , Proteínas do Nucleocapsídeo , Domínios e Motivos de Interação entre Proteínas , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Ressonância de Plasmônio de Superfície , Valina/química , Replicação Viral
2.
Int J Biol Macromol ; 51(4): 597-603, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22750578

RESUMO

A protein-bound polysaccharide (GSP-4) with a molecular weight of 8.3 × 105 Da, was isolated from the water extract of the fruiting bodies of Ganoderma sinense. Chemical study revealed that this fraction was composed of mannose, glucose and galactose in the molar ratio of 4.7:27.1:1.0, with the sugar residues of t-, 1,3-, 1,4-, 1,6-, 1,3,4- and 1,3,6-linked Glcp, t-linked Galp, and 1,6-linked Manp. The immnomodulatory effects of GSP-4 were assessed using human peripheral blood mononuclear cells (PBMCs) and murine monocyte/macrophage cell line RAW 264.7. We found that GSP-4 could significantly stimulate the production of the immunomodulatory markers tumor necrosis factor α (TNF-α), interleukin (IL)-1ß, IL-12, and granulocyte-macrophage colony-stimulating factor (GM-CSF) in PBMCs. This observation was further substantiated in RAW 264.7 cells, as indicated by the increase of nitric oxide (NO), TNF-α and IL-6 production. GSP-4 also enhanced the expression of inducible NO synthase mRNA in dose-dependent manner. Our current finding gives the first piece of evidence to support that GSP-4 possesses some promising immunomodulating effects and it could be a potential candidate to be further used in related cancer immunotherapy.


Assuntos
Ganoderma/química , Fatores Imunológicos/química , Fatores Imunológicos/farmacologia , Polissacarídeos/química , Polissacarídeos/farmacologia , Animais , Linhagem Celular , Humanos , Fatores Imunológicos/isolamento & purificação , Leucócitos Mononucleares/efeitos dos fármacos , Leucócitos Mononucleares/imunologia , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Camundongos , Óxido Nítrico/biossíntese , Polissacarídeos/isolamento & purificação
3.
J Agric Food Chem ; 60(17): 4276-81, 2012 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-22500548

RESUMO

A polysaccharide (GSP-6B) with a molecular mass of 1.86 × 106 Da was isolated from the fruiting bodies of Ganoderma sinense . Chemical composition analysis, methylation analysis, infrared spectroscopy, and nuclear magnetic resonance spectroscopy were conducted to elucidate its structure. GSP-6B contains a backbone of (1→6)-linked-ß-D-glucopyranosyl residues, bearing branches at the O-3 position of every two sugar residues along the backbone. The side chains contain (1→4)-linked-ß-D-glucopyranosyl residues, (1→3)-linked-ß-D-glucopyranosyl residues, and nonreducing end ß-D-glucopyranosyl residues. An in vitro immunomodulating activity assay revealed that GSP-6B could significantly induce the release of IL-1ß and TNF-α in human peripheral blood mononuclear cell (PBMC) and showed no toxicity to either PBMC or a human macrophage cell line THP-1. GSP-6B could also activate dendritic cells (DC) by stimulating the secretion of IL-12 and IL-10 from DC.


Assuntos
Carpóforos/química , Ganoderma/química , Fatores Imunológicos/farmacologia , Polissacarídeos/química , Polissacarídeos/farmacologia , Configuração de Carboidratos , Linhagem Celular , Células Dendríticas/efeitos dos fármacos , Células Dendríticas/imunologia , Humanos , Interleucina-10/metabolismo , Interleucina-12/metabolismo , Interleucina-1beta/metabolismo , Leucócitos Mononucleares/efeitos dos fármacos , Leucócitos Mononucleares/imunologia , Estrutura Molecular , Fator de Necrose Tumoral alfa/metabolismo
4.
Chemosphere ; 80(4): 366-74, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20494398

RESUMO

In this work, the homogeneous and heterogeneous degradations of diphenamid (DPA) in aqueous solution were conducted by direct photolysis with UVC (254nm) and by photocatalysis with TiO(2)/UVA (350nm), and the experimental results were compared. It was found that the homogeneous photolysis by UVC irradiation alone was quite efficient to degrade DPA up to 100% after 360min, but was very inefficient to mineralize its intermediates in terms of dissolved organic carbon reduction of only 8%. In contrast, the heterogeneous photocatalysis with TiO(2)/UVA showed relatively a lower degree of DPA degradation (51%), but a higher degree of its mineralization (11%) after 360min. These results reveal that the photocatalysis process has relatively poor selectivity to degrade different compounds including various intermediates from the DPA degradation, which is beneficial to its mineralization. In addition, over 20 intermediates were identified by LC-MS and (1)H NMR analyses. Based on the identified intermediates, the reaction mechanisms and the detailed pathways of the DPA degradation by photolysis and photocatalysis were proposed, and are presented in this paper.


Assuntos
Ácidos Difenilacéticos/química , Fotólise , Poluentes Químicos da Água/química , Catálise , Ácidos Difenilacéticos/efeitos da radiação , Recuperação e Remediação Ambiental , Titânio/química , Raios Ultravioleta , Água/química , Poluentes Químicos da Água/efeitos da radiação
5.
Chemosphere ; 73(5): 805-12, 2008 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-18640697

RESUMO

In this study, the highly-ordered TiO(2) nanotube (TNT) arrays on titanium sheets were prepared by an anodic oxidation method. Under UV illumination, the TNT films demonstrated the higher photocatalytic activity in terms of 2,3-dichlorophenol (2,3-DCP) degradation in aqueous solution than the conventional TiO(2) thin films prepared by a sol-gel method. The effects of dissolved oxygen (DO) and pH on the photocatalytic degradation of 2,3-DCP were investigated. The results showed that the role of DO in the 2,3-DCP degradation with the TNT film was significant. It was found that 2,3-DCP in alkaline solution was degraded and dechlorinated faster than that in acidic solution whereas dissolved organic carbon removal presented an opposite order in dependence of pH. In the meantime, some main intermediate products from 2,3-DCP degradation were identified by a (1)H NMR technique to explore a possible degradation pathway. A major intermediate, 2-chlororesorcinol, was identified from the 2,3-DCP decomposition as a new species compared to the findings in previous reports. Photocatalytic deactivation was also evaluated in the presence of individual anions (NO(3)(-), Cl(-), SO(4)(2-), and H(2)PO(4)(-)). The inhibition degree of photocatalytic degradation of 2,3-DCP caused by these anions can be ranked from high to low as SO(4)(2-)>Cl(-)>H(2)PO(4)(-)>NO(3)(-). The observed inhibition effect can be attributed to the competitive adsorption and the formation of less reactive radicals during the photocatalytic reaction.


Assuntos
Clorofenóis/química , Nanotubos/química , Oxigênio/química , Fármacos Fotossensibilizantes/química , Titânio/química , Ânions/química , Catálise , Concentração de Íons de Hidrogênio , Fotoquímica
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