Identification of potential anti-tumor targets and mechanisms of HuaChanSu injection using network pharmacology and cytological experiments in Breast cancer.

HuaChanSu (HCS) or Cinobufacini injection is an aqueous extract of the dried skin of Bufo bufo gargarigans, and has anti-tumor effects. The aim of this study was to evaluate the possible therapeutic effect of HCS against breast cancer (BRCA) using cytology, network pharmacology, and molecular biology approaches. The half-inhibitory concentration (IC50) of HCS in the BRCA cells was determined by cytotoxicity assay, and were accordingly treated with high and low doses HCS in the TUNEL and scratch assays. The potential targets of HCS in the BRCA cells were identified through functional enrichment analysis and protein-protein interaction (PPI) networks, and verified by molecular docking. The expression levels of key signaling pathways-related proteins in HCS-treated BRCA cells by western blotting. HCS inhibited the proliferation and migration of MCF-7 and MDA-MB-231 cells, and induced apoptosis in a dose-dependent manner. Furthermore, we screened 289 core HCS targets against BRCA, which were primarily enriched in the PI3K-AKT, MAPK chemokines, and other. signaling pathways. In addition, PIK3CA, PIK3CD, and MTOR were confirmed as HCS targets by molecular docking. Consistent with this, we observed a reduction in the expression levels of phosphorylated PI3K, AKT, and MTOR in the HCS-treated BRCA cells. Taken together, our findings suggest that HCS inhibits the growth of BRCA cells by targeting the PI3K-AKT pathway, and warrants further investigation as a therapeutic agent for treating patients with BRCA.

Green- and Blue-Emitting Tb3+-Activated Linde Type A Zeolite-Derived Boro-Aluminosilicate Glass for Deep UV Detection/Imaging.

Tb3+-activated LTA zeolite-derived boro-aluminosilicate glass samples with a composition of xTb2O3-68(Na2O-Al2O3-SiO2)-32B2O3 (x = 0.2, 1.0 and 10 extra wt%) were prepared using the melt-quenching method. The emission spectra recorded upon ultraviolet (UV) excitation with two different wavelengths of 193 and 378 nm showed blue light (5D3 to 7FJ=6,5,4 and 5D4 to 7F6 transitions of Tb3+) and green light (5D4 to 7F5 transition of Tb3+) emissions with comparable intensities up to a Tb3+ concentration of 10 extra wt%. Of note, the mean decay times of the green luminescence of the glass samples were relatively fast (<20 µs). The synthesized glass has potential in applications concerning UV imaging, UV detection, and plasma display panels.

Capacitance-soaring phenomenon induced by CuO electrode reconstruction with metastable Cu(OH)2 nanowires.

Metastable Cu(OH)2 nanowire precursors are reconstructed to interconnected CuO nanosheets through cyclic voltammetry. The capacitance retention rate of the electrode increases to 320% of its original value at a current density of 60 mA cm-2 after 10 000 charge and discharge cycles, and a strange capacitance-soaring phenomenon occurs. Thus, it can be inferred that the structural reconstruction and phase transition of the metastable phase of the electrode during the electrochemical process may also improve the electrochemical performance, which necessitates the reconsideration of the non-stable state of the electrode. Furthermore, it should not be uniformly labeled as unacceptable.

Robust superhydrophobic diamond microspheres for no-loss transport of corrosive liquid microdroplets.

Superhydrophobic surfaces usually lose their characteristics when exposed to a corrosive environment. To solve this issue, we synthesized superhydrophobic diamond microspheres by microwave-plasma-assisted chemical vapor deposition. Commercial epoxy glue was used to bond the microspheres to various substrates. The thus-synthesized composite films exhibited robust superhydrophobicity and an ultrahigh adhesive force.

[Effects of Chansu injection on transplanting-tumor models S180 in mice and human colon cancer HT-29 in nude mice].

OBJECTIVE: To study the antitumor effects of Chansu injection on transplanting- tumor of S(180 ) in mice and human colon cancer HT-29 in nude mice. METHODS: Using transplanting- tumor models of S(180 ) in mice and human colon cancer HT-29 in nude mice,the tumor inhibitive ratio(IR) of Chansu injection was determined and apoptosis was microscopically observed. RESULTS: Compared with tumor-negative control groups, IR at different dosage of Chansu in models of S(180) and HT-29 was 19.1% - 38.2% and 9.5% - 15.8% respectively,there was a dose-dependent relationship in models of S ( 180) (P< 0.05) and HT- 29 (P> 0.05). The tumor growth was markedly inhibited by cyclophosphamide (CTX) in model of S( 180) with IR of 70.7% and in model of HT-29 with IR of 67.1%, compared with control groups, both P< 0.01; apoptosis induced by CTX was markedly observed by in microscope examination. No significant side effects were shown in the study group. CONCLUSIONS: Chansu injection can significantly inhibit tumor growth in model of S(180), but not in model of HT- 29. Different type of tumor has different drug-sensitivity.