RESUMO
OBJECTIVE: To investigate the feasibility and technology for purifying Ganodermna lucidum polysaccharide (GLP) by ultrafiltration. METHODS: A spire roll ultrafiltration unit was selected to purify GLP. RESULTS: Purity of 73.5% and recovery ratio of 69.4% for GLP were obtained by using a membrane with cut-off molecular mass of 6000, while operating temperature, pressure and time were 45-50 degrees C, 0.6 MPa and 45 min, respectively. CONCLUSION: Ultrafiltration is one of the new feasible methods for the purification of GLP.
Assuntos
Antineoplásicos/isolamento & purificação , Polissacarídeos/isolamento & purificação , Reishi/química , Ultrafiltração/métodos , Antineoplásicos/química , Estudos de Viabilidade , Peso Molecular , Polissacarídeos/química , Pressão , Espectrofotometria Ultravioleta , Esporos Fúngicos/química , Temperatura , Fatores de Tempo , Ultrafiltração/instrumentaçãoRESUMO
OBJECTIVE: To isolate and purify Chlorogenic acid from Eucommia ulmoides leaves by ultrafiltration technique. METHODS: Chlorogenic acid transferring ratio was investigated by temperature, pressure, time, cut-off molecular mass. RESULTS: The optimal conditions for the ultrafiltration technique were finally determined. It was found that, for the membrane with a cut-off molecular mass of 20000, when the temperature, pressure, time were respectively 40 degrees C, 0.36 MPa, 20 minutes and adding 24L water, yield of Chlorogenic acid was 99.38%. CONCLUSION: High Chlorogenic acid transfering ratio can be gotten by ultrafiltration technique, which is helpful to the further isolation and purification of Chlorogenic acid in the subsequent process.
Assuntos
Ácido Clorogênico/isolamento & purificação , Medicamentos de Ervas Chinesas/isolamento & purificação , Eucommiaceae/química , Plantas Medicinais/química , Tecnologia Farmacêutica/métodos , Ácido Clorogênico/química , Medicamentos de Ervas Chinesas/química , Concentração de Íons de Hidrogênio , Peso Molecular , Folhas de Planta/química , Pressão , Temperatura , Ultrafiltração/métodosRESUMO
The extraction technique of ultrasonic wave on increasing the triterpeneclucoside was studied. Based on single factor experiments and the orthogonal design introduced,the optimized extracting conditions of triterpeneclucoside were investigated. The results showed that the best extraction technique conditons are 65% alcohol-water, the ratio of solid to liquid 1:30,dousing over 30 minutes,ultrasonic wave lasting for 40 minutes, ultrasonic frequency 26.8 KHZ, ultrasonic (output) power 1080 kw and extraction temperature 40 degrees C, respectively.
