Application of Bayes Probability Model in Differentiation of Yin and Yang Jaundice Syndromes in Neonates / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To study the application of Bayes probability model in differentiating yin and yang jaundice syndromes in neonates.</p><p><b>METHODS</b>Totally 107 jaundice neonates who admitted to hospital within 10 days after birth were assigned to two groups according to syndrome differentiation, 68 in the yang jaundice syndrome group and 39 in the yin jaundice syndrome group. Data collected for neonates were factors related to jaundice before, during and after birth. Blood routines, liver and renal functions, and myocardial enzymes were tested on the admission day or the next day. Logistic regression model and Bayes discriminating analysis were used to screen factors important for yin and yang jaundice syndrome differentiation. Finally, Bayes probability model for yin and yang jaundice syndromes was established and assessed.</p><p><b>RESULTS</b>Factors important for yin and yang jaundice syndrome differentiation screened by Logistic regression model and Bayes discriminating analysis included mothers' age, mother with gestational diabetes mellitus (GDM), gestational age, asphyxia, or ABO hemolytic diseases, red blood cell distribution width (RDW-SD), platelet-large cell ratio (P-LCR), serum direct bilirubin (DBIL), alkaline phosphatase (ALP), cholinesterase (CHE). Bayes discriminating analysis was performed by SPSS to obtain Bayes discriminant function coefficient. Bayes discriminant function was established according to discriminant function coefficients. Yang jaundice syndrome: y1= -21. 701 +2. 589 x mother's age + 1. 037 x GDM-17. 175 x asphyxia + 13. 876 x gestational age + 6. 303 x ABO hemolytic disease + 2.116 x RDW-SD + 0. 831 x DBIL + 0. 012 x ALP + 1. 697 x LCR + 0. 001 x CHE; Yin jaundice syndrome: y2= -33. 511 + 2.991 x mother's age + 3.960 x GDM-12. 877 x asphyxia + 11. 848 x gestational age + 1. 820 x ABO hemolytic disease +2. 231 x RDW-SD +0. 999 x DBIL +0. 023 x ALP +1. 916 x LCR +0. 002 x CHE. Bayes discriminant function was hypothesis tested and got Wilks' λ =0. 393 (P =0. 000). So Bayes discriminant function was proved to be with statistical difference. To check Bayes probability model in discriminating yin and yang jaundice syndromes, coincidence rates for yin and yang jaundice syndromes were both 90% plus.</p><p><b>CONCLUSION</b>Yin and yang jaundice syndromes in neonates could be accurately judged by Bayesian discriminating functions.</p>

Photolyses of mammalian oxy-hemoglobin studied by nanosecond photoacoustic calorimetry.

Enthalpy and conformational volume changes in photolyses of oxy-hemoglobin (HbO(2)) of human, bovine, pig, horse and rabbit are investigated by photoacoustic calorimetry. In the experiment, a pulsed Nd:YAG laser is used as an exciting source, and a PVDF film transducer and a PZT transducer are used to detect the photoacoustic signals. Based on the time scales of the excitation and detection systems as well as the photolysis processes of HbO(2), it can be indicated that the measured enthalpy and conformational volume changes are related to slow geminate recombination and tertiary relaxation in photolyses of HbO(2), which are with the time scale of 30-40 ns and 100-150 ns, respectively. The results show that the enthalpy and conformational volume changes are different for both photolysis processes of HbO(2) and also for various mammals. The different results among the five mammals are analyzed and discussed briefly.

Photo-dissociation quantum yields of mammalian oxyhemoglobin investigated by a nanosecond laser technique.

The photo-dissociations of oxyhemoglobin of several mammals, such as human, bovine, pig, horse, and rabbit, have been studied. By means of optical pump-probe technique, the quantum yields for photo-dissociation of these oxyhemoglobin have been determined at pH 7 and 20 degrees C. A nanosecond laser at 532 nm is used as the pumping source, and a xenon lamp through a monochrometer provides a probe light at 432 nm. The experimental results show that the quantum yields of these mammalian oxyhemoglobin are different from each other, especially for that of rabbit. By analyzing the amino acid sequences and tetramer structures as well as the flexibility and hydrophobicity of the different hemoglobin, possible explanations for the differences are proposed.

Enthalpy and conformational volume changes of mammalian oxy-hemoglobins investigated by pulsed photoacoustic calorimetry.

Enthalpy and conformational volume changes induced by laser photo-dissociation reactions of mammalian oxy-hemoglobin, such as human, bovine, pig, horse and rabbit oxy-hemoglobins, are investigated by pulsed photoacoustic calorimetry. Generally, the response time of the photoacoustic calorimetry is restricted by the width of the laser pulse and the bandwidth of the acoustic detector. Considering the time window of the experimental system, the enthalpy and conformational volume changes detected should be caused by the tertiary relaxation of the heme proteins. In order to calculate the enthalpy and conformational volume changes, the quantum yields of the photo-dissociation products of oxy-hemoglobins must be measured and taken into account. Finally, the enthalpy and conformational volume changes of the oxy-hemoglobins connected with the tertiary relaxation are obtained, which show that for all measured mammalian oxy-hemoglobins the values of enthalpy changes are in the range of 30.0-46.8 kcal/mol and volume changes are of 2.3-7.8 ml/mol although the quantum yields for the different species may have much bigger differences. A possible explanation of the results is presented.