Effect of Microvascular Invasion on the Prognosis in Hepatocellular Carcinoma and Analysis of Related Risk Factors: A Two-Center Study.

Objective: Microvascular invasion is considered to initiate intrahepatic metastasis and postoperative recurrence of hepatocellular carcinoma (HCC). We aimed to analyze the effect of MVI on the prognosis in HCC and identify related risk factors for microvascular invasion (MVI). Methods: The clinical data of 553 HCC patients who underwent liver surgery at Qingdao University from January 2014 to December 2018 and 89 patients at Beijing Tsinghua Changgung Hospital treated between October 2014 and October 2019 were collected retrospectively. We explored the impact of MVI on the prognosis of patients with HCC using Kaplan-Meier analysis. We conducted logistic regression analysis to identify variables significantly related to MVI. Results: Pathological examination confirmed the presence of MVI in 265 patients (41.3%). Six factors independently correlated with MVI were incorporated into the multivariate logistic regression analysis: Edmondson-Steiner grade [odds ratio (OR) = 3.244, 95%CI: 2.243-4.692; p < 0.001], liver capsule invasion (OR = 1.755; 95%CI: 1.215-2.535; p = 0.003), bile duct tumor thrombi (OR = 20.926; 95%CI: 2.552-171.553; p = 0.005), α-fetoprotein (> 400 vs. < 400 ng/ml; OR = 1.530; 95%CI: 1.017-2.303; p = 0.041), tumor size (OR = 1.095; 95%CI: 1.027-1.166; p = 0.005), and neutrophil-lymphocyte ratio (OR = 1.086; 95%CI: 1.016-1.162; p = 0.015). The area under the receiver operating characteristic curve (AUC) was 0.743 (95%CI: 0.704-0.781; p < 0.001), indicating that our logistic regression model had significant clinical usefulness. Conclusions: We analyzed the effect of MVI on the prognosis in HCC and evaluated the risk factors for MVI, which could be helpful in making decisions regarding patients with a high risk of recurrence.

Chemical constituents from Lagotis brevituba / 中国中药杂志

<p><b>OBJECTIVE</b>To study on the chemical consitituents of Lagotis brevituba.</p><p><b>METHOD</b>The chemical consitituents were isolated by silica gel column chromatography, polyamide column chromatography and semi-preparative HPLC, and their structures were identified by spectroscopic methods.</p><p><b>RESULT</b>Eight compounds were isolated and they were identified as beta-sitosterol (1), succinic acid (2), luteolin-7-O-beta-D-glucoside (3), uracil (4), apigenin (5), chrysoeriol (6), chrysoeriol-7-O-beta-D-glucoside (7), and apigenin-7-O-beta-D-glucoside (8).</p><p><b>CONCLUSION</b>Compound 4-8 were isolated from L. brevituba for the first time, and among them, compound 7 and 8 were isolated from genus Lagotis for the first time.</p>

A new phenylethanoid glucoside from Lagotis brevituba / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of Lagotis brevituba.</p><p><b>METHOD</b>The chemical constituents were isolated and purified by silica gel column chromatography, polyamide column chromatography, and semi-preparative HPLC, and their structures were elucidated on the basis of analysis of IR,NMR, 2D-NMR, and MS spectra.</p><p><b>RESULT</b>Two compounds were obtained and were identified as phenylethanoid glucosides, lagotiside A (1) and acteoside (2), respectively.</p><p><b>CONCLUSION</b>Compound 1 is a new compound and named as lagotiside A.</p>

Determination of lignans of Schisandra rubriflora by HPLC / 中草药

Object To determine the contents of schisantherin A, deoxyschizandrin, schisandrin B and schisandrin C in the stems and fruits of Schisandra rudriflora (Franch ) Rehd. et Wils , which were collected from different areas Methods An HPLC method was set up: Column, Sphereclone, ODS (250 mm?4 6 mm, 5 ?m); mobil phase, A: H 2O, B: MeOH; gradient elution was with 70% B from 0-4 min, 70%-100% B from 4-54 min; the flow rate was 0 4 mL/min; the column temperature was 25 ℃ and the DAD detector was used at 254 nm Results The contents of schisantherin A, deoxyschizandrin, schisandrin B and schisandrin C in S. rubriflora were 0.026%-0.083%, 0.007%-0.945%, 0.002%-0.121%, 0.010%-0.038%, respectively. Deoxyschizandrin exists widely in S. rubriflora and its content is higher in fruits than that in stems Conclusion Deoxyschizandrin is the main active lignan in the fruits of S rubriflora

Pharmacognostical identification of Chinese crude drug Fructus Schisandrae Sphenantherae / 中草药

Object To provide a basis for the identification of the crude drug of Fructus Schisandrae Sphenantherae Methods Morpholgical characters and microstructural features of the seed surface of Schisandra sphenanthera Rehd. et Wils , S. rubriflora (Franch ) Rehd. et Wils., S. viridis A. C. Smith, and S. henryi Clarke were observed under light microscope and scanning electron microscope. Identification of them was also completed by TLC qualitative analysis for deoxyschisandrin and schisantherin A Results The fruits of S. sphenanthera, S. rubriflora, S. viridis and S. henryi could be classified to three types with the characters of micromorphological features of the seed surface. The results of TLC showed that deoxyschisandrin and schisantherin A were present in the fruits of S. sphenanthera from Pingli, Shaanxi, Luanchuan, Henan, Yangcheng, Shanxi, S. rubriflora and S.viridis, Hengshan Hunan Provinces The fruit of S. henryi contained a little quantity of schisantherin A, but the fruits of S. sphenanthera from Liuba Shaanxi and Xiaolongshan Gansu Provinces did not have deoxyschisandrin and schisantherin A. Conclusion The fruits of S. sphenanthera from different geographical origin, S. rubriflora, S. viridis and S. henryi can be identified by the characters of micromorphological featrues of the seed surface and the TLC qualitative analysis for deoxyschisandrin and schisantherin A