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1.
Poult Sci ; 103(4): 103506, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38335672

RESUMO

Avian reovirus (ARV), which commonly induces viral arthritis or tenosynovitis and immunosuppression in chickens, is associated with the nonstructural protein p17 that plays a crucial role in viral replication and regulates cellular signaling pathways through its interaction with cellular proteins. In our previous study, we identified the host protein IFN-γ-inducible protein-16 (IFI16) as an interacting partner of ARV p17 through yeast two-hybrid screening. In the current study, we further confirmed the interaction between IFI16 and p17 protein using coimmunoprecipitation, glutathione S-transferase (GST)-pulldown assay, and laser confocal microscopy techniques. Additionally, we found that the amino acid of p1761-119 is responsible for mediating the interaction with the HINa and HINb domains of IFI16. Interestingly, we observed a significant increase in IFI16 expression upon ARV infection or p17 protein exposure. Moreover, the replication of ARV was found to be largely influenced by the quantity of IFI16 protein. Overexpression of IFI16 led to a significant decrease in ARV replication, while knockdown of the IFI16 expression led to the contrary result. Additionally, our findings demonstrate that IFI16 plays a crucial role in the induction of inflammatory cytokines IFN-ß and IL-1ß during ARV infection as confirmed by qRT-PCR and ELISA analyses. In conclusion, our study provides novel insights into the functional role of p17 protein and the pathogenic mechanism underlying ARV infection, particularly its association with inflammatory response. Furthermore, it offers new perspectives for identifying potential therapeutic targets against ARV infection.


Assuntos
Orthoreovirus Aviário , Animais , Chlorocebus aethiops , Orthoreovirus Aviário/genética , Galinhas , Replicação Viral , Células Vero , Terapia de Imunossupressão/veterinária
2.
Virus Res ; 320: 198886, 2022 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-35948130

RESUMO

The classical swine fever virus (CSFV) is one of the most harmful pathogens of swine and causes considerable economic loss. Mitophagy is a selective form of autophagy that degrades damaged mitochondria by combining with lysosomes. Previous studies have been reported that CSFV infection can induce mitophagy, but which effector protein is responsible for this process remains unclear. Herein, we revealed here that the CSFV nonstructural protein 5A (NS5A) plays a critical role in inducing cellular mitophagy. Specifically, the expression of CSFV NS5A in the PK-15 cells induces membrane potential loss and mitochondrial fission, and the quantities of mitophagosomes, the expression of Parkin and PINK1 were significantly increased compared with mock cells. Intriguingly, we found that Parkin-overexpression promotes CSFV propagation. Furthermore, the expression level of reactive oxygen species (ROS) was increased by CSFV NS5A protein, while NS5A-induced mitophagy correlated with the quantity of ROS production. In summary, our results reveal a new function of NS5A in inducing cellular mitophagy and broaden our understanding of the mechanism of CSFV-induced mitophagy, which may provide a new way to develop an antiviral strategy.


Assuntos
Vírus da Febre Suína Clássica , Peste Suína Clássica , Animais , Mitofagia , Espécies Reativas de Oxigênio/metabolismo , Suínos , Ubiquitina-Proteína Ligases/metabolismo , Proteínas não Estruturais Virais/genética , Proteínas não Estruturais Virais/metabolismo , Replicação Viral
3.
Virology ; 541: 75-84, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-32056717

RESUMO

Classical swine fever is a world organization for animal health listed disease and is caused by classical swine fever virus (CSFV). CSFV can induced unfolded protein response (UPR) and whether NS5A protein plays a role in this process remains unknown. Here, we demonstrate that CSFV induced all the three signal pathways ATF6, IRE1 and PERK of UPR. Furthermore, this phenomenon may be mediated by the NS5A protein since expression of NS5A alone can achieve the same effect. In the current study, we show that NS5A can interact with GRP78 as measured by using the CO-IP and GST pulldown assays. This interaction plays a positive role in the promotion of CSFV replication. Overexpression or knockdown of GRP78 mediated by lentivirus can enhance or decrease viral replication, respectively. Our findings provide the evidence that CSFV infection can activate the cellular UPRs, in which NS5A and GRP78 play key roles in the process.


Assuntos
Vírus da Febre Suína Clássica/fisiologia , Resposta a Proteínas não Dobradas/fisiologia , Proteínas não Estruturais Virais/fisiologia , Replicação Viral , Chaperona BiP do Retículo Endoplasmático , Células HEK293 , Proteínas de Choque Térmico/fisiologia , Interações entre Hospedeiro e Microrganismos , Humanos , Transdução de Sinais/fisiologia
4.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-810414

RESUMO

Echinococcosis is an age-old disease that causes serious damage to the animal husbandry and the human health perennially. As a newly discovered species of Echinococus, E. shiquicus has the potential public health significance and could be a potential parasitic zoonosis. In this review, its etiology, life cycle, epidemiology, detection and diagnoses, public health etc. are discussed or summarized. Also, a series of comparisons among E. granulosus, E. multilocularis and E. shiquicus are made.

5.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-410525

RESUMO

Objective To evaluate adbomen surgery in patients who are found to have poorly controlled or untreated hypertension before operation.Methods From January 1997 to December 1998,the perioperative clinical data of 531 patients with hypertension and undergoing abdomen surgery in our hospital were retrospectively analyzed.Results The incidences of perioperative hypertensive events were not significantly different,between controlled and uncontrolled patients with grade one and grade two (P>0.05).In grade three and systolic hypertension,certain perioperative complications in patients with poorly controlled hypertension were higher than in those with well-controlled hypertension(P<0.05).Conclusion The patients with grade on e and grade two hypertension are not at increased operative risk.In Patients with grade three and systolic hypertension,perioperative complications are increased and elective surgery should be postponed until their blood pressure is brought under 180/110 mmHg over 1 to 2 weeks.

6.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-531112

RESUMO

Objective To investigate the effect of glucagon-like peptide-1(GLP-1) in a dose related manner on glucose metabolism after 65% hepatectomy in rats.Methods We determined the serum glucose levels of hepatomized rats at 0,5,10,20,and 30 minutes after an intravenous glucose load(IVGTT,0.5 g/kg glucose) on the first postoperative day,and the changes of blood glucose,serum insulin and glucagon concentrations of hepatomized rats that received the volum load with normal saline or 0.3 nmol/kg GLP-1,0.45 nmol/kg GLP-1 respectively.Blood was drawn for determination of glucose(glucose oxidase),insulin,glucagon,and GLP-1(radioimmunoassay).Results The peak glucose and 30-minute glucose levels and the area under the curve(AUC 0-30) were significantly higher in the hepatomized rats compared to the control rats,which had not undergone any operation and received a same intravenous glucose load(0.5 g/kg glucose with normal saline)(P0.05).Nevertheless the peak glucose and 30-minute glucose levels and AUC 0-30 of the hepatomized rats that received with 0.45 nmol/kg GLP-1 were significantly lower compared to the rats that received the same volum load with normal saline or 0.3 nmol/kg GLP-1 respectively after liver resection.There was an increasing postoperative serum concentration of glucose,insulin,glucagon on the first day,then,the serum glucose concentration was significantly lowered after infusion of GLP-1 in rats undergoing hepatectomy(P

7.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-529297

RESUMO

0.05).Conclusions After hepatectomy,in rats is associated with an obvious insulin resistance,mainly due to the level of serum insulin was obviously decreased(P

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