Adiponectin exerts antiproliferative effect on high glucose-induced BeWo cell proliferation in vitro / 中华妇产科杂志

Objective To determine the effects of adiponectin on high glucose induced BeWo cell proliferation in vitro. Methods BeWo cells were seeded in 96-well plates at the appropriate density. After treatments with high glucose (25 mmol/L), western blot analysis of cyclin D1 and a colorimetric assay (cell counting kit-8, CCK-8) were used to analyse BeWo cells′proliferation, and western blot was used to detect the expression of adiponectin. Moreover, we added adiponectin (20μg/ml) in the culture medium and three methods were utilized for cell proliferation analysis: CCK-8, cell cycle analysis (by flow cytometry) and proliferating cell nuclear antigen (PCNA) immunocytochemical staining. Results Compared to BeWo cells cultured by normal glucose and high mannitol, the proliferation of BeWo cells treated by high glucose increased (P<0.05). Compared with BeWo cells cultured by high mannitol, the expression of adiponectin in BeWo cells treated by high glucose decreased. After added adiponectin in the culture medium, the proliferation of BeWo cells treated by adiponectin+high glucose decreased than that of cells treated by high glucose (0.770±0.050 versus 0.990±0.070, P<0.05);the proportion of G2+S phases of BeWo cells treated by adiponectin+high glucose decreased than that of cells treated by high glucose [(40.7±2.1)%versus (44.9± 3.9)%, P<0.05];the rate of PCNA positive cell in BeWo cells treated by adiponectin+high glucose decreased than that of cells treated by high glucose [(28 ± 5)% versus (44 ± 5)%, P<0.05]. Conclusion Adiponectin could inhibit proliferation of high glucose induced BeWo cells in vitro.

Diabetes Insipidus in childhood Analysis of 74 cases / 实用儿科临床杂志

74 cases of diabetes insipidus were reported in this paper. There were polydipsia,polyuris and low specific gravity of urine in all patients. Classification was determinedas follows: pituidary 64 cases (including idiopathic 44 cases, secondary 20 cases),nephritic 8 cases and unclassified 2 cases. Intracranial tumors should be paid altentionto rule out. 12 patients responded very satisfactorily to oral administration of singlechlorpropamide. In order to avoid hypoglycemia, an initjal dose of 10 mg/kg/24hr wasrecommended.