Assessment of UV radiation effects on airborne mucormycetes and bacterial populations in a hospital environment.

Infections, such as mucormycosis, often result from inhaling sporangiospore present in the environment. Surprisingly, the extent of airborne Mucormycetes sporangiospore concentrations remains inadequately explored. This study aimed to assess the influence of UV radiation on microbial populations and Mucormycetes spore levels within a hospital environment in northern Iran. A comprehensive dataset comprising 298 air samples collected from both indoor and outdoor settings was compiled. The culture was conducted using Blood Agar and Dichloran Rose Bengal Chloramphenicol (DRBC) culture media, with Chloramphenicol included for fungal agents and Blood Agar for bacterial. Before UV treatment, the average count of Mucormycetes ranged from 0 to 26.4 ± 25.28 CFU m-3, fungal agents from 2.24 ± 3.22 to 117.24 ± 27.6 CFU m-3, and bacterial agents from 29.03 ± 9.9 to 359.37 ± 68.50 CFU m-3. Following UV irradiation, the averages were as follows: Mucormycetes ranged from 0 to 7.85 ± 6.8 CFU m-3, fungal agents from 16.58 ± 4.79 to 154.98 ± 28.35 CFU m-3, and bacterial agents from 0.38 ± 0.65 to 43.92 ± 6.50 CFU m-3. This study, notably marks the pioneering use of UV light to mitigate Mucormycetes spore counts and bacterial agents in northeastern Iran, contributing to the advancement of environmental health and safety practices in hospital settings.

Plasma immobilization of azobenzene dye on polyamide 6 polymer.

Plasma treatment of polymeric materials is a cost-effective and efficient technique to modify the surface and change the constituent unit configuration. This research investigates the effects of argon DC glow discharge plasma on pure and DR1 dye-loaded polyamide 6 polymer films and stabilization of dye on the surface. Plasma breaks some bonds and activates the surface through creating reactive structures such as free radical sites on the surface and increases tertiary amides on the surface of polymer. Besides, this process alters surface topographical characteristics and conformation of azobenzene dye which are effective on the durability of the dye on the surface. Plasma causes interactions of the dye with the polymer and immobilizes the dye on the polymer. On the other hand, these interactions lead to changes in the dye's optical and geometric isomeric activity and stability. This work studies the chemical and morphological changes of polyamide 6 by plasma with AFM and spectroscopic methods. Furthermore, the aging of nylon 6 films loaded with DR1 dye is measured, and the conformational changes of the dye are investigated. Plasma stabilizes the dye on the polymer surface through making changes of chemical and physical properties on the surface components.

Glow discharge plasma stabilization of azo dye on PMMA polymer.

The effects of argon gas glow discharge plasma on the surface of DR1 dye-loaded PMMA polymer films are examined in this work. Plasma immobilizes the dye on the surface of polymer without using stabilizers. Argon plasma activates the surface through breaking some bonds and generation of radical sites. It affects the acrylate groups of PMMA leading to covalent bonds between dye and surface of polymer. In addition, plasma treatment and contact with ambient air may result in the creation of new polar components, such as carbonyl and carboxyl compounds and links that enhance the dye attachment to the polymer matrix. Besides, the dye adsorption on the polymer film is impacted by changes in surface topography. Furthermore, plasma modifies the dye conformation, which affects the adherence of the dye to the polymer surface through bringing the dye to the higher energy state. The chemical and topographical modification of dye-loaded PMMA films by plasma are investigated by spectroscopic and AFM methods. Furthermore, aging process was used to confirm dye retention on the polymer film after plasma modification as opposed to dye-loaded polymer film that was left untreated as a reference sample. Finally, investigated method suggests a novel and very affordable technique for fabrication of poly(MMA-co-DR1) copolymer in the form of a homogeneous surface layer.