Safety Assessment: a Comparative Analysis of Quantitative Content of Bacterial Endotoxins and Evaluation of Pyrogenicity of the Kazakhstan Vaccine QazCovid-in® against COVID-19.

We measured the levels of bacterial endotoxins in the bulk vaccine product (BVP) and finished vaccine QazCovid-in® and evaluated the effect of aluminum hydroxide (adjuvant) on the results of LAL test and pyrogenicity of samples in vivo (in rabbits receiving intravenous injection into the marginal ear vein). Administration of BVP with LPS resulted in a dose-dependent increase in body temperature in rabbits similar to that caused by LPS alone, which suggests that aluminum hydroxide in the vaccine did not affect the pyrogenic response in rabbits. Moreover, the LAL test showed that the aluminum hydroxide did not hinder LPS activity after serial dilution of samples.

Biochemical Analysis of the Blood of Cattle after Vaccination with a Heterologous Vaccine for Lumpy Skin Disease.

We studied the biochemical parameters of the blood of cattle after immunization against lumpy skin disease with a vaccine made from an attenuated heterologous goat pox virus (strain G20-LKV). Blood samples were obtained from animals on days 7, 14, and 21 after vaccination. The vaccine did not affect biochemical parameters of the blood. A slight increase in total protein and AST on day 14 indicates the expected reactions to the stimulation of the immune system after vaccination. The levels of direct and total bilirubin, ALT, urea, glucose, cholesterol, and creatinine in vaccinated animals remained within the physiological limits.

Research progress on the material basis and pharmacological effects of Pien Tze Huang / 药学学报

The medicinal history of Pien Tze Huang is long, and it is the only "double top secret" variety of technology and formula at present. It has the effects of clearing heat and detoxifying, detumescence and pain, cooling blood and removing blood stasis. At present, researchers have analyzed and identified some compounds in Pien Tze Huang and its precious medicinal materials, such as Panax notoginseng, calculus bovis, snake gall and musk, and conducted activity screening, pharmacokinetics and pharmacological related studies on these chemical components. It was found that Pien Tze Huang had a significant effect on the treatment of acute and chronic hepatitis, ulcer, colon cancer, liver cancer and other diseases. The purpose of this paper is to systematically discuss the research achievements of researchers in recent years on the material basis, pharmacological effects and clinical application of Pien Tze Huang, with a view to providing ideas for the further research of Pien Tze Huang.

[Clinicopathological and molecular features of multinodular and vacuolating neuronal tumors of the cerebrum].

Objective: To investigate clinicopathological features of multinodular and vacuolar neurodegenerative tumor (MVNT) of the cerebrum, and to investigate its immunophenotype, molecular characteristics and prognosis. Methods: Four cases were collected at the General Hospital of Southern Theater Command, Guangzhou, China and one case was collected at the First People's Hospital of Huizhou, China from 2013 to 2021. Clinical, histological, immunohistochemical and molecular characteristics of these five cases were analyzed. Follow-up was carried out to evaluate their prognoses. Results: There were four females and one male, with an average age of 42 years (range, 17 to 51 years). Four patients presented with seizures, while one presented with discomfort on the head. Pre-operative imaging demonstrated non-enhancing, T2-hyperintense multinodular lesions in the deep cortex and superficial white matter of the frontal (n=1) or temporal lobes (n=4). Microscopically, the tumor cells were mostly arranged in discrete and coalescent nodules primarily within the deep cortical ribbon and superficial subcortical white matter. The tumors were composed of large cells with ganglionic morphology, vesicular nuclei, prominent nucleoli and amphophilic or lightly basophilic cytoplasm. They exhibited varying degrees of matrix vacuolization. Vacuolated tumor cells did not show overt cellular atypia or any mitotic activities. Immunohistochemically, tumor cells exhibited widespread nuclear staining for the HuC/HuD neuronal antigens, SOX10 and Olig2. Expression of other neuronal markers, including synaptophysin, neurofilament and MAP2, was patchy to absent. The tumor cells were negative for NeuN, GFAP, p53, H3K27M, IDH1 R132H, ATRX, BRG1, INI1 and BRAF V600E. No aberrant molecular changes were identified in case 3 and case 5 using next-generation sequencing (including 131 genes related to diagnosis and prognosis of central nervous system tumors). All patients underwent complete or substantial tumor excision without adjuvant chemoradiotherapy. Post-operative follow-up information over intervals of 6 months to 8 years was available for five patients. All patients were free of recurrence. Conclusions: MVNT is an indolent tumor, mostly affecting adults, which supports classifying MVNT as WHO grade 1. There is no tumor recurrence even in the patients treated with subtotal surgical excision. MVNTs may be considered for observation or non-surgical treatments if they are asymptomatic.

[Carbon Emission Reduction Effect of Spatial Correlation Network Structure Characteristics: From the Perspective of Urban Agglomeration].

Based on social network and regression analyses, this study conducted research with regard to the spatial correlation and effect of carbon emissions in urban agglomerations of the Pearl River Delta (PRD) with the data of prefecture-level cities in Pearl River Delta urban agglomerations from 2001 to 2019. As the results suggested, ① the carbon emission links among cities in PRD urban agglomerations were relatively loose, the subordinate structure changed frequently, and the carbon emission exchanges and cooperation among cities need to be strengthened. ② The core-periphery structure of the spatial correlation network of carbon emissions was loose to a certain extent, the control ability of the central city to the overall network structure was weakened, and the marginal cities gradually changed from being controlled to adopting a controlling role. ③ The improvement in the overall network tightness and stability and the breaking of the network hierarchical structure significantly reduced regional carbon emissions, whereas the improvement in individual network centrality significantly promoted urban carbon emission reduction. The above analysis will help to provide reference for coordinating carbon dioxide emission reduction and low-carbon city development policies.

Effect of transition metal cations on the local structure and lithium transport in disordered rock-salt oxides.

Lithium-excess oxides Li1.2Ti0.4Mn0.4O2 and Li1.3Nb0.3Mn0.4O2 with a disordered rock-salt structure and Mn3+/Mn4+ as a redox couple were compared to analyze the effect of different d0 metal ions on the local structure and Li+ ion migration. These cathode materials were obtained by mechanochemically assisted solid-state synthesis. Using XRD, 7Li NMR and EPR spectroscopy and transmission electron microscopy it was shown that the Mn ions are prone to form clusters, while d0 metal ions are evenly distributed in the crystal lattice. The presence of Nb5+ ions contributes to the formation of noticeably larger Mn clusters and larger gaps in the Li+ migration maps as compared to Ti4+. These results were confirmed by the geometrical-topological method, BVSE simulation and DFT calculations, and are in good agreement with the Li diffusion coefficient determined by GITT, which is 1.5 orders of magnitude higher in Li1.2Ti0.4Mn0.4O2 than that in Li1.3Nb0.3Mn0.4O2.

Extravasation of chemotherapeutic drug from an implantable intravenous infusion port in a child: A case report.

BACKGROUND: Drug extravasation is a complication of totally implantable access port (TIAP) use and could cause tissue injury and sustained organ dysfunction. Therefore, the clinical management of children with TIAP is challenging. CASE SUMMARY: This was a case of extravasation of a chemotherapeutic drug (paclitaxel) from an implantable infusion port in a 23-mo old child. After fully evaluating the skin at the site of extravasation, the nurse continued to use the infusion port to complete the follow-up chemotherapeutic course. The skin around the infusion port was red, and showed no ulceration, swelling, or induration at discharge. CONCLUSION: Since children are more active and often noncompliant, it is necessary to appropriately train pediatric nurses caring for individuals with TIAPs, and any abnormal situation should be timely addressed.

[Analysis of the diagnosis of occupational radiogenic neoplasms in three cases of leukemia].

Objective: To analyze the diagnosis of 3 cases of leukemia applying for the diagnosis of occupational radiogenic neoplasms. Methods: Retrospective analysis the occupational history, the disease history and the probability of causation (PC value) information of 3 radiological workers. Results: Two cases' PC value of 95% confidence limit of were >50%, and they were diagnosed as radiogenic neoplasms. One case was <50% and diagnosed as nonoccupational radiogenic neoplasms. Conclusion: The probability of causation analysis has important guiding significance for the diagnosis of occupational radiogenic neoplasms. Radiological workers should improve their awareness of self-protection and reduce the occurrence of occupational diseases.

Evaluation of microstructure and conductivity of two-phase materials by the scanning spreading resistance microscopy (the case of shungite).

The determination of the content of the conducting phase and the assessment of conductivity by microscopic images are interesting for rapid and non-destructive analysis of the electrophysical properties of two-phase (conductor/dielectric) samples during the atomic force microscopy. In this paper we summarized results of the analysis of the conductivity maps of the shungite surface by the method of discretization by applying a square grid with subsequent binary digital processing. Microstructure and conductivity were evaluated by measuring the average length of continuous conductive circuits isolated on the grid. A model was considered that established a unique correspondence (up to normalizing coefficients) between the length of the conductive circuits on the conductivity maps and the integral conductivity of the sample as a whole. An analytical equation was obtained that described such dependence with an accuracy of units of percent. We proposed a method for measuring the integral conductivity of a shungite samples based on an analysis of its binary conductivity map obtained by spreading resistance microscopy. This method can be used to determine the conductivity by surface conductivity mapping for shungite-like two-phase conductor/dielectric systems, and in general, for any two-phase substances where the phases differ in AFM-determined properties.

High-effective biosynthesis of baccatin â ¢ by using the alternative acetyl substrate, N-acetyl-d-glucosamine.

AIMS: Paclitaxel is a type of broad-spectrum anticancer drug in short supply. The price of acetyl-CoA (17 709 677·4 USD mol-1 ), which is the acetyl group donor for the enzymatic synthesis of the intermediate, baccatin Ⅲ, is still the bottleneck of the mass production of paclitaxel. This study reports a novel acetyl group donor, which could substantially reduce the cost of production. METHODS AND RESULTS: In this study, a substrate spectrum with 14 kinds of representative acetyl-donor substitutes predicted by computer-aided methods was tested in a 10-deacetylbaccatin Ⅲ-10-O-acetyltransferase (DBAT) heterogeneous-expressed open-whole-cell catalytic system. The results of computer prediction and experimental analysis revealed the rule of the acetyl-donor compounds based on this substrate spectrum. N-acetyl-d-glucosamine (30·95 USD mol-1 , about 572 202-fold cheaper than acetyl-CoA) is selected as a suitable substitute under the rule. The yield when using N-acetyl-d-glucosamine as acetyl donor in open-whole-cell catalytic system was 2·13-fold of that when using acetyl-CoA. In the in vivo system, the yield increased 24·17%, which may indicate its cooperation with acetyl-CoA. CONCLUSION: The success of open-whole-cell synthesis and in vivo synthesis of baccatin Ⅲ by adding N-acetyl-d-glucosamine as acetyl substrate demonstrates that it is a useful substrate to improve the yield of baccatin Ⅲ. SIGNIFICANCE AND IMPACT OF THE STUDY: All these findings provided a potential acetyl-donor substitute for acetyl-CoA, as well as a low cost and efficient method of preparing paclitaxel through baccatin Ⅲ semi-synthesis.

[The new approach in the treatment of walking disorders in Parkinson's disease]. / Novyi podkhod k lecheniiu narushenii khod'by pri bolezni Parkinsona.

AIM: To evaluate the efficacy of a proprietary method based on stimulation of the hindfoot when lifting the foot from the surface of the support and bringing it forward in the treatment of walking disorders in patients with Parkinson's disease (PD). MATERIAL AND METHODS: The study included 19 patients with walking disorders, akinetic-rigid and mixed form of PD. The stage of the disease on the Hoehn-Yahr scale at the time of the study was 2.56±0,41. During the treatment of walking disorders using the proprietary method, correction of anti-parkinsonian therapy was not carried out. Correction of walking disorders was carried out by using 'activating platforms', which allow mechanical stimulation of the hindfoot at the moment of lifting the foot from the surface of the support. Neurological examination, computer stabilometry (CS), gait function evaluation by means of the LA-1 laser analyzer of kinematic parameters of gait, Berg Balance Scale (BBS), Dynamic Gait Index (DGI), Unified Parkinson's Disease Rating Scale (UPDRS) were used before and after treatment. RESULTS: A significant decrease in core values of LA-1 and positive dynamics on BBS, DGI, UPDRS was revealed after treatment. CONCLUSION: The original method based on stimulation of foot lifting from the surface and moving the foot forward, effectively improves the pattern of walking in patients with PD and can be recommended as a non-pharmacological method of treatment of impaired walking at the third stage of PD.

[Impact of L-carnitine and phosphatidylcholine containing products on the proatherogenic metabolite TMAO production and gut microbiome changes in patients with coronary artery disease].

The aim of the study was to assess the impact of L-carnitine and phosphatidylcholine containing products on the production of the proatherogenic metabolite TMAO and gut microbiome changes in patients with coronary artery disease (CAD). Material and methods. The study consisted of 2 parts. In the first part, a comparison was made between the diet of patients with CAD (n=29) and healthy volunteers (n=30) over the age of 50 with respect to the frequency of intake of L-carnitine and phosphatidylcholine containing products. All participants underwent blood sampling and stool tests to assess the concentration of TMAO and the composition of fecal microflora. The second part of the study was dedicated to assessing the correlation between TMAO blood concentration in patients with CAD (n=89) and the frequency of intake of L-carnitine and phosphatidylcholine containing products. Results and discussion. Patients with CAD comparing to healthy people among the predecessor products of TMAO consumed red meat, dairy products more often, eggs and fish less often. TMAO concentration in patients with CAD was higher than in healthy volunteers (1036.4±748.2 vs 376.5±147.9 ng/ml, p=0.0001). Analysis of fecal microflora in patients with CAD revealed an increase number of bacteria from Verrucomicrobiaceae family (p<0.05) and Enterobacteriaceae family (p<0.05), of the Escherichia/Shigella genera (p<0.05), there was a trend to increased number of Ruminococcus (р=0.065), Clostridium XlV (b) genera (р=0.10). Correlation between TMAO concentration and frequency of red meat, eggs, and dairy products consumption was estimated in patients with CAD (r>0.525, р<0.05). Conclusion. Patients with CAD consume more precursors of TMAO, have higher blood TMAO concentrations compared to healthy volunteers. Fecal microflora of patients with CAD contains a greater number of gut bacteria related to trimethylamine producers compared to healthy volunteers. Reducing the number of L-carnitine and phosphatidylcholine containing products in the diet of patients with CAD may affect the decrease in the proatherogenic metabolite TMAO concentration.

Discovery of a Lead Triphenylethanamine Cholesterol Ester Transfer Protein (CETP) Inhibitor.

Lead optimization of the diphenylpyridylethanamine (DPPE) and triphenylethanamine (TPE) series of CETP inhibitors to improve their pharmaceutical profile is described. Polar groups at the N-terminus position in the DPPE series resulted in further improvement in potency and pharmaceutical properties concomitant with retaining the safety, efficacy, and pharmacokinetic (PK) profile. A structure-activity relationship observed in the DPPE series was extended to the corresponding analogs in the more potent TPE series, and further optimization resulted in the identification of 2-amino-N-((R)-1-(3-cyclopropoxy-4-fluorophenyl)-1-(3-fluoro-5-(1,1,2,2-tetrafluoroethoxy)phenyl)-2-phenylethyl)-4,4,4-trifluoro-3-hydroxy-3-(trifluoromethyl)butanamide (13). Compound 13 demonstrated no significant changes in either mean arterial blood pressure or heart rate in telemetry rats, had an excellent PK profile, and demonstrated robust efficacy in human CETP/apo-B-100 dual transgenic mice and in hamsters.

Processing of household waste in the BTLSS using the wet combustion method.

The present study discusses physicochemical methods of organic waste processing in closed biotechnical life support systems (BTLSS). Sanitary and household cotton wastes were processed by the method of wet combustion in hydrogen peroxide using an alternating current electric field - a promising physicochemical method for organic waste processing in the BTLSS. The highest efficiency of the process (in terms of power consumption, duration of the process, and oxidation rate) was achieved in experiments with oxidation of a combination of cotton fabrics and urea-containing wastes such as human urine and feces. The reason for this must be that urea is a reactive aqueous solvent of cellulose.

[Costanzo Varolio (1543-1575) - a significant personality in the development of neuroanatomy]. / Kostantso Varolio (1543-1575) - znachimaia lichnost' v istorii neiroanatomii.

The biography of outstanding physician and anatomist Costanzo Varolio (1543-1575) is considered. His contribution to the study of the structure of the nervous system is characterized. The authors have translated fragments of his works concerning a method of brain dissection and pons later named in his honor. They point out that eponym 'pons Varolii' had been coined by Thomas Bartholin in his 'Historiarum anatomicarum rariorum centuria' in 1654.

Feasibility of incorporating all products of human waste processing into material cycling in the BTLSS.

The present study addresses the ways to increase the closure of biotechnical life support systems (BTLSS) for space applications. A promising method of organic waste processing based on "wet combustion" in hydrogen peroxide developed at the IBP SB RAS to produce fertilizers for higher plants is discussed. The method is relatively compact, energy efficient, productive, and eco-friendly. However, about 4-6 g/L of recalcitrant sediment containing such essential nutrients as Ca, Mg, P, Fe, Cu, Mn, and Zn precipitates after the initial process. These elements are unavailable to plants grown hydroponically and, thus, drop out of the cycling as dead-end products. Possible methods of dissolving that sediment have been studied. Results of experiments show that the most promising method is additional oxidation of the sediment in HNO3 + H2O2. By using the new technological process, which only involves substances synthesized inside the BTLSS material flows, more than 90% of each nutrient can be converted into the form available to plants in irrigation solutions, thus returning them into the material cycling. The results obtained in this study show the efficacy of supplementing the irrigation solutions with the mineral nutrients after sediment dissolution. Lettuce plants grown as the test object on the newly prepared irrigation solutions produced the yield that was more than twice higher than the yield produced on the nutrient solutions prepared without the sediment conversion into a soluble form. Composition of the gases emitted during this process has been analyzed. Dynamics of oxidation of the small fractions of a wax-like sediment remaining after the initial sediment dissolution in HNO3 + H2O2 in the BTLSS soil-like substrate has been studied. The entire technological scheme aimed at the full inclusion of all human wastes into the BTLSS cycling has been suggested and discussed. A process scheme of including products of human waste processing in the biotic cycle of the BTLSS is discussed in the conclusion.

PK/PD Disconnect Observed with a Reversible Endothelial Lipase Inhibitor.

Screening of a small set of nonselective lipase inhibitors against endothelial lipase (EL) identified a potent and reversible inhibitor, N-(3-(3,4-dichlorophenyl)propyl)-3-hydroxy-1-methyl-2-oxo-1,2-dihydropyridine-4-carboxamide (5; EL IC50 = 61 nM, ELHDL IC50 = 454 nM). Deck mining identified a related hit, N-(3-(3,4-dichlorophenyl)propyl)-4-hydroxy-1-methyl-5-oxo-2,5-dihydro-1H-pyrrole-3-carboxamide (6a; EL IC50 = 41 nM, ELHDL IC50 = 1760 nM). Both compounds were selective against lipoprotein lipase (LPL) but nonselective versus hepatic lipase (HL). Optimization of compound 6a for EL inhibition using HDL as substrate led to N-(4-(3,4-dichlorophenyl)butan-2-yl)-1-ethyl-4-hydroxy-5-oxo-2,5-dihydro-1H-pyrrole-3-carboxamide (7c; EL IC50 = 148 nM, ELHDL IC50 = 218 nM) having improved PK over compound 6a, providing a tool molecule to test for the ability to increase HDL-cholesterol (HDL-C) levels in vivo using a reversible EL inhibitor. Compound 7c did not increase HDL-C in vivo despite achieving plasma exposures targeted on the basis of enzyme activity and protein binding demonstrating the need to develop more physiologically relevant in vitro assays to guide compound progression for in vivo evaluation.

Blood hammer phenomenon in human aorta: Theory and modeling.

This paper contains the results of computing of a blood pressure and a flow speed in a human aorta in a diastolic phase of a heart cycle. The model is based on the one-dimensional flow approach. The blood hammer effect means abrupt increasing of pressure in a blood vessels due to the sharp changes in flow speed. The closing of aortic valve at the proto-diastole phase causes such blood hammer. We consider an aorta as a simple cylindrical conduit with elastic walls. The aortic valve and the bifurcation were located in the opposite ends of the conduit. The analysis of possible types of blood hammer effect in the "conduit-blood" system was performed. The lifelike initial and boundary conditions for the problem were proposed. We found a strong peak of pressure during the first third of diastole at the normal closure of the aortic valve. We observed the minor fluctuations of pressure in the later part of diastole too. Blood flow speed also has minor oscillations during the diastole. Such results are typical under the complete blood hammer effect condition. An abnormal long valve closure causes an incomplete blood hammer effect. In that case the calculated oscillations of the flow speed had higher intensity without strong pressure peak. The Fourier spectra of pressure fluctuations are located in the range of 16-87 Hz, that is nearby to known frequencies of the second heart sound produced by aortic valve.

Preconception antiphospholipid antibodies and risk of subsequent early pregnancy loss.

Objectives To prospectively estimate the association of preconception antiphospholipid antibodies (aPL) with subsequent pregnancy loss using a cohort design. aPL have been associated with recurrent early pregnancy loss (EPL) prior to 10 weeks in previous case-control studies. Prospective ascertainment of pregnancy loss is challenging, as most women do not seek care prior to EPL. Methods Secondary analysis of the Effects of Aspirin in Gestation and Reproduction trial of preconception low-dose aspirin. Preconception anticardiolipin (aCL) and anti-ß2-glycoprotein-I (a-ß2-I) were assessed in 1208 women with one or two prior pregnancy losses and no more than two prior live births. Comparison cohorts were defined by positive aPL (+aPL) or negative aPL (-aPL) status. All women were followed for six menstrual cycles while trying to conceive; if successful, they underwent an ultrasound at 6-7 weeks' gestation. EPL was defined as loss prior to 10 weeks' gestation; embryonic loss was loss after visualization of an embryo but prior to 10 weeks; clinical loss was any loss after visualization of an embryo (with or without fetal cardiac activity detected). Results In total, 14/1208 (1%) tested positive for +aPL. 786/1208 (65%) women had positive human chorionic gonadotropin during the study period, of which 9/786 (1%) had +aPL. Of the 786 pregnant women, 589 (75%) had live births and 24% had pregnancy losses. Women with +aPL experienced EPL at similar rates as women with -aPL, 44% vs 21% (aRR 2.4, 95% confidence interval (CI) 0.5-10.9). Embryonic loss was more common in women with +aCL IgM (aRR 4.8, 95% CI 1.0-23.0) and in women with two positive aPL. Clinical pregnancy loss was more common in women with positive a-ß2-I IgM (50% vs 16.5%, aRR 3.7, 95% CI 1.3-10.8). Conclusion Positive levels of aPL are rare in women with one or two prior pregnancy losses and are not clearly associated with an increased rate of subsequent loss. Clinical trial registration The original source study was registered at ClinicalTrials.gov (#NCT00467363).

[Infection Status of Human Papilloma Virus,Ureaplasma Urealyticum, Chlamydia Trachomatis,and Neisseria Gonorrhoeae].

Objective To analyze the infection status of human papilloma virus (HPV),Ureaplasma urealyticum (UU),Chlamydia trachomatis (CT),and Neisseria gonorrhoeae (NG) in clinical patients.Methods The laboratory specimens including urine,urethral swabs,and cervical swabs from 870 patients from January 1st 2014 to December 31st 2017 were retrospectively analyzed. HPV-DNA was detected by multiplex fluorescent PCR,and the UU-RNA,CT-RNA,and NG-RNA were determined by isothermal nucleic acid amplification. The positive rate of each pathogen and the distribution of positive rate between male and female patients were calculated. The samples were further divided into HPV-positive group and HPV-negative group,and the positive rates of UU-RNA,CT-RNA,and NG-RNA in these two groups were compared.Results The highest positive rate was 53.68%(467/870) for UU-RNA,followed by HPV-DNA [32.41%(282/870) ]and NG-RNA [2.18%(19/870)]. The total positive rate of high-risk (HR)-HPV(subtypes:16,18,31,33,35,39,45,51,52,56,58,59,and 68) [31.52%(209/663)]and UU in female patients [60.93%(404/663)] was significantly higher than that in male patients [17.39%(36/207),30.34%(63/207)](both P<0.001). The male patients had significantly higher CT positive rate in HR-HPV-positive group than in HR-HPV-negative group [22.58%(7/31) vs. 4.54%(8/176)](P<0.001). The female patients had significantly higher CT positive rate in HR-HPV-positive group than in HR-HPV-negative group [10.5%(21/200) vs. 5.61%(26/463)](P=0.024). The UU-RNA positive rate of females in the low-risk (LR)-HPV (subtypes:6 and 11) positive group was significantly higher than that in LR-HPV negative group [70.83%(34/48) vs.2.11%(13/615)](P<0.001).Conclusions Women are more susceptible to HR-HPV and UU infections. HR-HPV-positive patients are more likely to experience CT infection. In contrast,co-infection with UU is more common in LR-HPV-positive females.