RESUMO
We have developed a prototype ocular imaging system that integrates optical-resolution photoacoustic microscopy and high-frequency ultrasound imaging. The system can perform high-resolution ocular imaging from the anterior region down to the fundus. It has successfully imaged murine eyes in vivo, including iris, lens, retina, and retinal pigment epithelium. Our results demonstrate that this system shows strong potential for the diagnosis of ophthalmic diseases.
Assuntos
Técnicas de Diagnóstico Oftalmológico/instrumentação , Microscopia Acústica/métodos , Técnicas Fotoacústicas/métodos , Animais , Desenho de Equipamento , Olho/anatomia & histologia , Olho/diagnóstico por imagem , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Microscopia Acústica/instrumentação , Modelos Animais , Dispositivos Ópticos , Fenômenos Ópticos , Técnicas Fotoacústicas/instrumentaçãoRESUMO
In this work, a nanoscale reduced graphene oxide-iron oxide nanoparticle (RGO-IONP) complex is noncovalently functionalized with polyethylene glycol (PEG), obtaining a RGO-IONP-PEG nanocomposite with excellent physiological stability, strong NIR optical absorbance, and superparamagnetic properties. Using this theranostic nanoprobe, in-vivo triple modal fluorescence, photoacoustic, and magnetic resonance imaging are carried out, uncovering high passive tumor targeting, which is further used for effective photothermal ablation of tumors in mice.
Assuntos
Grafite/química , Nanopartículas de Magnetita/química , Nanocompostos/química , Animais , Compostos Férricos/química , Imageamento por Ressonância Magnética , Nanopartículas de Magnetita/uso terapêutico , Camundongos , Nanocompostos/uso terapêutico , Neoplasias/diagnóstico , Neoplasias/terapia , Oxirredução , Óxidos/química , Fototerapia , Polietilenoglicóis/químicaRESUMO
Zebrafish play an important role in biological and biomedical research. Traditional in vivo imaging methods for studying zebrafish larvae primarily require fluorescence labeling. In this work, relying on tissue intrinsic optical absorption contrast, we acquired high resolution label-free 3D images of zebrafish larvae by using photoacoustic microscopy (PAM) in vivo. The spatial resolution reaches several microns, allowing the study of microstructures in various living organs. We demonstrated that our method has the potential to be a powerful non-invasive imaging method for studying various small animal models, including zebrafish larvae, Caenorhabditis elegans, frogs and drosophila larvae.
