DOCK8 Serves as a Prognostic Biomarker and Is Related to Immune Infiltration in Patients With HPV Positive Head and Neck Squamous Cell Carcinoma.

PURPOSE: Dedicator of cytokinesis 8 (DOCK8) was reported to have a vital link to immunoregulation. However, the mechanisms by which it drives immune infiltration in cancer remain uncertain. We tried to assess the role of DOCK8 in patients with cancer, especially human papillomavirus (HPV)-positive head and neck squamous cell carcinoma (HNSCC). METHODS: Data on the expression and survival of DOCK8 in patients with various cancers were analyzed using the Oncomine and TIMER databases. The TIMER database assessed the relationship of DOCK8 with immune infiltration levels and various markers of multiple immune cells. Gene set enrichment analysis revealed tumor-associated biological processes related to DOCK8. ENCODE database was used to explore relevant transcription factors of DOCK8, and a PPI network was constructed using GENEMINIA. The expression and survival role of DOCK8 was confirmed in patients from independent GEO datasets. RESULTS: We determined that DOCK8 expression was upregulated or downregulated in various cancers unlike in healthy tissues. A high expression of DOCK8 was significantly correlated with a favorable prognosis in HPV-positive HNSCC and lung adenocarcinoma (LUAD). Furthermore, multivariate Cox regression analysis revealed that DOCK8 was an independent prognostic factor of HPV-positive HNSCC. Additionally, elevated DOCK8 expression was positively correlated with multiple immune cell infiltration levels and immune marker expression associated with particular immune cell subsets. Also, 14 pathways involved in immune activities and carcinogenesis, 22 potential TFs, and co-expression proteins of DOCK8 indicated DOCK8 to be related to tumor-associated biological processes. Ultimately, we verified that DOCK8 is upregulated and confers a favorable overall survival and progression-free survival status in patients with HPV-positive HNSCC. CONCLUSION: These results elucidate that high expression of DOCK8 indicates a favorable prognosis in patients with HPV-positive HNSCC as well as increased microenvironmental immune infiltration levels. It would provide new insights into the prognosis predicting and clinical regimen decision making in patients with HPV-positive HNSCC.

Agreement of young adults and orthodontists on dental aesthetics & influencing factors of self-perceived aesthetics.

BACKGROUND: The aim of this study is to assess the agreement between orthodontist's and Chinese young adult's self-perceived aesthetics, the normative treatment need based on the Index of Orthodontic Treatment Need (IOTN), and the main factors affecting the self-perceived aesthetics. METHODS: A random sample of 348 Chinese young adults (116 males and 232 females) aged 17-24 years were recruited in this study. Two orthodontists were involved in rating the cases. Orthodontic treatment need was assessed according to the Index of Orthodontic Treatment Need, including Aesthetic components and Dental Health Components (AC and DHC). Personality traits were assessed according to Eysenck Personality Questionnaire (EPQ). Cohen's kappa test was used to assess the agreement, and spearman's correlation coefficient was used to analyze the association among all variables. RESULTS: A statistically significant level of agreement was observed between young adult's perception and orthodontist's perception in IOTN (kappa = 0.14). A positive relationship (p < 0.001)existed between the young adult's AC and the orthodontist' s AC (r = 0.275), and between the young adult' s AC and the normative need DHC (r = 0.195). The orthodontist's AC was strongly related to the normative need (r = 0.743, p < 0.001). Association between the young adult's AC and gender and EPQ-E were also observed. CONCLUSIONS: Young adults tend to be less critical in assessing orthodontic treatment needs than orthodontists. The orthodontist's AC reflecting subjective treatment need is strongly connected to the normative need. The adult's perception of aesthetic component is affected by factors such as gender and personality traits.

Effect of personality on oral health-related quality of life in undergraduates.

OBJECTIVES: To evaluate the effects of personality traits and other variables on oral health-related quality of life (OHRQoL) of Chinese undergraduates aged 17 to 24 years with no history of orthodontic treatment. MATERIALS AND METHODS: A total of 443 undergraduate students aged 17 to 24 years were selected and completed a two-section questionnaire: the Eysenck Personality Questionnaire Short Scale Chinese version and the Oral Health Impact Profile-14 Chinese version (OHIP-14). Malocclusion severity was assessed via clinical examination using the dental health component (DHC) and esthetic component of the Index of Orthodontic Treatment Need (IOTN). Severe dentofacial deformities that required orthognathic surgery were excluded. RESULTS: The total and all dichotomized OHIP-14 dimensions correlated closely with the neuroticism personality traits ( P < .001). The total and some subscales of OHIP-14 were related with psychoticism. Women had higher OHIP-14 scores than men, especially in the normal occlusion group. Nevertheless, men with more severe malocclusions had higher scores that were similar to those of women. OHRQoL was significantly different between the major of dentistry and other majors. CONCLUSIONS: Personality affects comprehensive OHRQoL, and the most significant predictor is neuroticism. Female young adults have worse OHRQoL than male young adults, especially among those with mild malocclusion.