RESUMO
OBJECTIVES: This study describes a novel carrier, the ethosome-based system, which is composed of non-ionic surfactants, ethanol, and water. METHODS: Brij(®) 52 (non-ionic surfactants), soya phosphatidylcholine (PC), cholesterol, and the major compounds (caffeine and gallic acid) of black tea extracts were dissolved in the ethanolic phase. The aqueous phase containing Paragon III was heated to 60 °C and mixed with the previous solution. Finally, 3.4 ml NaOH (6.5 N) was added to adjust the pH level to 4.05. The mixture was centrifuged at 2000 g for two minutes, and the precipitate was taken as the end product. Black tea extracts were applied in ethosome-based formulations, and the efficacy of these formulations in penetrating nude mouse skin and in dyeing white hairs was investigated. RESULTS: Compared with an ethanolic solution and black tea extracts, the non-ionic ethosomal delivery system dramatically enhanced the adsorption of black tea extracts onto hair surfaces in vitro. The non-ionic ethosomal system was much more efficient in delivering and facilitating the adsorption of black tea extracts to the hair surface than hydroalcoholic black tea extracts. CONCLUSIONS: This formulation may have potential for development as a hair dye and protective agent.
Assuntos
Cafeína/farmacocinética , Portadores de Fármacos/farmacocinética , Ácido Gálico/farmacocinética , Tinturas para Cabelo/farmacocinética , Cabelo/metabolismo , Extratos Vegetais/farmacocinética , Pele/metabolismo , Adsorção , Animais , Cafeína/química , Cetomacrogol/química , Química Farmacêutica , Colesterol/química , Portadores de Fármacos/química , Etanol/química , Ácido Gálico/química , Tinturas para Cabelo/química , Camundongos , Camundongos Nus , Permeabilidade , Fosfatidilcolinas/química , Extratos Vegetais/química , Absorção Cutânea , Tensoativos/química , Chá , Lã/metabolismoRESUMO
OBJECTIVES: The primary objective of this study was to investigate the feasibility of using niosomes as a delivery vehicle for the dermal administration in vitro of black tea extract (BTE) as a sunscreen. METHODS: Multi-lamellar niosomes were obtained by means of a previously reported method of lipid hydration films. In vitro penetration experiments through nude mouse skin were carried out to evaluate the potential of niosomes as a dermal formulation. The nude mouse skin membrane allowed the effects of penetration with a niosome formulation to be evaluated. Penetration rates of caffeine- and gallic acid-loaded niosomes in a steady state were higher than dispersion in aqueous solutions. RESULTS: For skin permeation, higher transdermal absorption rates were seen with solutions of caffeine and gallic acid. CONCLUSIONS: In the near future, BTE as a sunscreen agent will be dermally delivered by niosomes.
