Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
J Environ Sci Health B ; 55(11): 951-958, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32698655

RESUMO

In this study, new polymers containing amides (TrisPS-Ntaa, and TrisPS-Ntaa-Fc) were synthesized by condensation reaction for qualitative identification of insecticides. The synthesized polymers, including amides were investigated by infrared spectroscopy (IR), scanning electron microscopy-energy dispersion X- ray spectrometry (SEM-EDX), and gel permeation chromatography (GPC). Then, acetylcholinesterase enzyme (AChE) was covalently immobilized on these polymers to improve properties (including activity, reusability, and storage stability). Accordingly, organophosphate (malathion, acephate, chlorpyrifos methyl) and carbamate (carbofuran, methiocarb, methomyl), which are used to prevent harmful organisms in some agricultural products were enzymatically determined based on their inhibitory activity on AChE.


Assuntos
Carbamatos/análise , Inseticidas/análise , Organofosfatos/análise , Polímeros/química , Acetilcolinesterase/química , Acetilcolinesterase/metabolismo , Amidas/química , Carbofurano/análise , Clorpirifos/análogos & derivados , Clorpirifos/análise , Inibidores da Colinesterase/análise , Inibidores da Colinesterase/farmacologia , Enzimas Imobilizadas/química , Enzimas Imobilizadas/metabolismo , Inseticidas/farmacologia , Malation/análise , Metomil/análise , Compostos Organotiofosforados/análise , Fosforamidas , Espectrometria por Raios X , Espectrofotometria Infravermelho
2.
Int J Mol Sci ; 13(9): 11870-11880, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23109888

RESUMO

Modified polystyrene with Pt(IV)-azomethine (APS-Sch-Pt) was synthesized by means of condensation and demonstrated to be a promising enzyme support by studying the enzymatic properties of glucose oxidase enzyme (GOx) immobilized on it. The characteristics of the immobilized glucose oxidase (APS-Sch-Pt-GOx) enzyme showed two optimum pH values that were pH = 4.0 and pH = 7. The insertion of stable Pt(IV)-azomethine spacers between the polystyrene backbone and the immobilized GOx, (APS-Sch-Pt-GOx), increases the enzymes' activity and improves their affinity towards the substrate even at pH = 4. The influence of temperature, reusability and storage capacity on the free and immobilized glucose oxidase enzyme was investigated. The storage stability of the immobilized glucose oxidase was shown to be eleven months in dry conditions at +4 °C.


Assuntos
Aspergillus niger/enzimologia , Compostos Azo/química , Enzimas Imobilizadas/química , Proteínas Fúngicas/química , Glucose Oxidase/química , Poliestirenos , Tiossemicarbazonas/química , Poliestirenos/síntese química , Poliestirenos/química
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...