RESUMO
The transforming growth factor ß (TGF-ß) superfamily plays critical roles in tumor suppression, cell proliferation and differentiation, tissue morphogenesis, lineage determination, cell migration and apoptosis. Recently, TGF-ß1, one important member of TGF-ß superfamily, is suggested as an immune regulator in the teleost. In this study, we cloned the cDNAs of TGF-ß1 and its receptors, TßR I and TßR II (including three isoforms) from tilapia (Genbank accession numbers: KP754231- KP754235). A tissue distribution profile analysis indicated that TGF-ß1 was highly expressed in the head kidney, gill, spleen, kidney and PBLs (peripheral blood leukocytes); TßR I only showed considerable expression in the liver; and TßR II-2 was highly expressed in the kidney, gill, liver, head kidney and heart. We determined that the mRNA expressions of TGF-ß and TßR I /TßR II-2 were significantly increased in tilapia head kidney and spleen leukocytes by the stimulation of Lipopolysaccharide (LPS) or Poly I: C. We also examined their expressions in the spleen and head kidney of tilapia after IP injection of streptococcus agalactiae. The results showed that the mRNA expressions of these three genes all increased in the head kidney as early as 6 h post infection, and in the spleen 3 d post infection. In addition, the protein level of TGF-ß1 was also up-regulated in the head kidney and the spleen after infection. Taken together, our data indicate that the TGF-ß1-TßR I /TßR II-2 system functions potentially in tilapia immune system.
