Comparing the effects of magnetite-mediated direct interspecies electron transfer with biogas mixing-driven interspecies hydrogen transfer on anaerobic digestion.

Although the promotive effect of direct interspecies electron transfer (DIET) on methane production has been well-documented, the practical applicability of DIET in different scenarios have not yet been systematically studied. This study compared the effects of magnetite-mediated DIET with conventional biogas mixing-driven interspecies hydrogen transfer (IHT) on anaerobic digestion (AD) of swine manure (SM). Compared with control, magnetite supplementation, biogas circulation, and their integration enhanced the CH4 yield by 19.3%, 25.9%, and 26.2%, respectively. Magnetite mainly enriched DIET-related syntrophic bacteria (Anaerolineae and Synergistia) and methanogens (Methanosarcina) to accelerate acidification and establish DIET, while biogas circulation mainly enriched hydrolytic bacteria (Clostridia) and hydrogenotrophic methanogens (Methanolinea and Methanobacterium) to promote hydrolysis and accelerate IHT. Coupling magnetite addition with biogas circulation led to the enrichment of the above six microorganisms to different extents. The effectiveness of the strategies for lowering the H2 pressure followed: magnetite + biogas circulation ≈ biogas circulation > magnetite. Under stress-free environment, the enhancement effect of magnetite-induced DIET was not even as pronounced as biogas circulation-a simple and common mixing strategy in commercial AD plants, and the promotion effect of magnetite was insignificant in the well-mixed digesters. In short, the magnetite-mediated DIET is not always effective in improving AD of SM.

Coupling Iron Sludge Addition and Intermittent Aeration for Achieving Simultaneous Methanogenesis, Feammox, and Denitrification in a Single Reactor Treating Fish Sludge.

An integrated anaerobic digestion system for the simultaneous removal of carbon and nitrogen from fish sludge was developed by coupling iron sludge supplementation with intermittent aeration. In terms of nitrogen removal, Fe(III) in iron sludge could trigger Feammox reactions and intermittent aeration could drive the Fe(II)/Fe(III) cycle to sustain continuous ammonia removal. Mass balance analysis suggested that nitrate was the main product of Feammox, which was subsequently removed through heterotrophic denitrification. In terms of carbon removal, the Fe(III)-induced dissimilatory iron reduction (DIR) process significantly promoted fish sludge hydrolysis and provided more simple organics for methanogens and denitrifiers, but aeration showed a negative impact on methanogenesis. To promote nitrogen removal and avoid serious methanogenesis inhibition, different aeration intensities were studied. Results showed that compared with the control without aeration or iron sludge addition, aeration for 5 min every 3 days (150 mL/min) contributed to a 29.0% lower NH4+-N concentration and a 12.1% lower total chemical oxygen demand level on day 28, and the decline in methane yield was acceptable (only 13.5% lower). Simultaneous methanogenesis, Feammox, and denitrification in a single reactor treating fish sludge were achieved, which provides a simple and low-cost strategy for the treatment of organic wastewater.

Synergistic effect of chlorhexidine and azoles on candida biofilm on titanium surface.

BACKGROUND: Candida infections of orthopedic implants are one of the most detrimental orthopedic implant-related complications with unsuccessful treatment and a poor prognosis. Most orthopedic Candida infections form biofilms and have resistance to the commonly used antifungal agents. This study aimed to develop a novel combination of normally prescribed drugs against Candida biofilm on orthopedic implants. METHODS: We cultured 26 clinical isolates of Candida strains to form biofilm without titanium sheets or on titanium sheets, which are the most commonly used materials for permanent or orthopedic implants. The checkerboard method was used to evaluate the synergistic effects of chlorhexidine (CHL) and azoles on these Candida biofilms. For the evaluation of synergistic effects, we constructed the cell viability assay by fluorescence staining and CFU reduction hot map of Candida. RESULTS: Twenty-six clinical isolates of Candida strains formed biofilm in 96-well plates without titanium sheets, and we selected 9 of them to form biofilm on titanium sheets in 24-well plates. In Candida biofilm formed in 96-wells, the synergistic rates of CHL with fluconazole, itraconazole, and voriconazole were 61% (16/26), 65% (17/26), and 23% (6/26), respectively. When compared to the blank control group, CHL monotherapy significantly inhibited biofilm formation on titanium sheets (P < 0.05). We demonstrated 100% synergistic rates of the CHL and fluconazole combination against Candida biofilm formation on titanium sheets, and the minimum inhibitory concentration of CHL and FLU decreased four- to eight-fold. CONCLUSIONS: We concluded that CHL combined with azoles inhibited the Candida biofilm formation 96-wells or on titanium sheets and has the potential to control the infections of orthopedic implants.

Biological nitrogen removal mechanisms during anaerobic digestion of swine manure: Effects of biogas circulation and activated carbon addition.

This study investigated the biological nitrogen removal mechanisms during the anaerobic digestion of swine manure and the effects of biogas circulation and activated carbon (AC) addition. Biogas circulation, AC addition, and their combination increased the methane yield by 25.9%, 22.3%, and 44.1%, respectively, when compared to the control. Nitrogen species analysis and metagenomic results indicated that nitrification-denitrification was the dominant ammonia removal pathway in all digesters with little oxygen, while anammox did not occur. Biogas circulation could promote mass transfer and induce air infiltration to enrich nitrification- and denitrification-related bacteria and functional genes. And AC might act as an electron shuttle to facilitate ammonia removal. The combined strategies showed a synergetic effect on the enrichment of nitrification and denitrification bacteria and functional genes, significantly lowering the total ammonia nitrogen by 23.6%. A single digester with biogas circulation and AC addition could enhance methanogenesis and ammonia removal via nitrification and denitrification.

Dissimilatory manganese reduction facilitates synergistic cooperation of hydrolysis, acidogenesis, acetogenesis and methanogenesis via promoting microbial interaction during anaerobic digestion of waste activated sludge.

Anaerobic digestion (AD) of waste activated sludge (WAS) is commonly limited to poor synergistic cooperation of four stages including hydrolysis, acidogenesis, acetogenesis and methanogenesis. Dissimilatory metal reduction that induced by metal-based conductive materials is promising strategy to regulate anaerobic metabolism with the higher metabolic driving force. In this study, MnO2 as inducer of dissimilatory manganese reduction (DMnR) was added into WAS-feeding AD system for mediating complicated anaerobic metabolism. The results demonstrated that main operational performances including volatile solid (VS) degradation efficiency and cumulative CH4 production with MnO2 dosage of 60 mg/g·VS reached up to maximum 53.6 ± 3.4% and 248.2 ± 10.1 mL/g·VS while the lowest operational performances in control group (38.5 ± 2.8% and 183.5 ± 8.5 mL/g·VS) was originated from abnormal operation of four stages. Furthermore, high-throughput 16 S rRNA pyrosequencing revealed that enrichment of dissimilatory manganese-reducing contributors and methanogens such as Thermovirga, Christensenellaceae_R_7_group and Methanosaeta performed the crucial role in short-chain fatty acids (SCFAs) oxidation and final methanogenesis, which greatly optimized operational environment of hydrolysis, acidogenesis and acetogenesis. More importantly, analysis of functional genes expression proved that abundances of genes encoding enzymes participated in acetate oxidation, direct interspecies electron transfer (DIET) and CO2 reduction pathway were simultaneously up-regulated with the optimum MnO2 dosage, suggesting that DMnR with SCFAs oxidation as electron sink could benefit stable operation of four stages via triggering effective DIET-based microbial interaction mode.

Removal Efficiency of Sulfapyridine from Contaminated Surface Water by Carboxylated Graphene Oxide Blended PVDF Composite Ultrafiltration Membrane with Activated Carbon.

In this study, sulfapyridine (SPY), an antibiotic that is less commonly treated by membrane filtration techniques but is frequently detected in the aqueous environment and at higher concentrations than other detected antibiotics, was selected for investigation. A composite ultrafiltration membrane for the removal of sulfapyridine (SPY) antibiotics from water was fabricated using polyvinylidene fluoride (PVDF), polyvinylpyrrolidone (PVP), and carboxyl-functionalized graphene oxide (CFGO) as additives. The changes in retention rate and pure water flux of sulfapyridine by the composite ultrafiltration membrane were investigated by changing the ratios of the prepared ultrafiltration membrane materials under the conditions of low-pressure operation to explore the optimal experimental conditions. The results showed that the addition of PVP and CFGO significantly increased the number of membrane pores and their pore size. The addition of CFGO in the membrane significantly improved the hydrophilicity of the membrane. The contact angle decreased from 83.7 to 31.6°. Compared to ordinary PVDF ultrafiltration membranes, the membrane's pure water flux increased nearly three times to 2612.95 L/(m2·h). The removal rate of SPY was 56.26% under the optimal conditions. When the composite ultrafiltration membrane was combined with activated carbon, the removal rate of SPY was 92.67%, which was nine times higher than that of activated carbon alone. At this time, the flux of the composite membrane was 2610.23 L/(m2·h). This study proposes a simple, efficient, and low production cost solution for the removal of sulfapyridine from water.

Comparison of pharmaceutical removal in two membrane bioreactors with/without powdered activated carbon addition.

The present study investigates the removal of six selected pharmaceuticals from municipal wastewater in two membrane bioreactors (MBRs) with and without powdered activated carbon (PAC) addition. Two approaches were carried out for obtaining different carbon dosages related to the influent: (1) with a fixed solids retention time (SRT) and varying PAC concentrations; (2) with varying SRTs and a fixed PAC concentration. The results reveal that a PAC dosage related to influent of 21 mg L-1 and SRT of 20 d are optimal. The first approach achieved a better removal performance than the second. The removal of amidotrizoic acid (up to 46%), bezafibrate (>92%) and iopromide (around 85%) were mainly caused by biological process, but were also enhanced by PAC addition. Efficient removal (>95%) of sulfamethoxazole, carbamazepine and diclofenac were highly dependent on the PAC dosage. However, carbamazepine shows re-metabolization properties during biological processing. Decreasing the SRT as done in the second approach, not only increased the PAC amount, but also decreased the mass of activated sludge and reduced the capability to degrade complex organic matter. Consequently, biodegradability and adsorbability played decisive roles in the removal of each compound.

Aerobic granular sludge cultivated from Fe-loaded activated carbon as carrier working low-strength wastewater conditions by bioreactor.

This study proposes a new method to promote the granulation process while accelerating the degradation efficiency of nutrients. The new strategy could involve preparing Fe-loaded activated carbon (FAC) before start-up of granular cultivation and then cultivating the process of aerobic granular sludge (AGS) with such materials. In addition, this experiment could further comprehend how the preparation and characteristics of FAC affect the formation and properties of AGS. The conclusions showed that compared with the control, FAC enhanced the sedimentation performance and significant removal efficiency. Meanwhile, the values of protein (PN) and polysaccharide (PS) also increased significantly in the addition of FAC, indicating the production of substances were induced by FAC. Molecular biology methods indicated that the rapid production of granulation and removal of nutrients were considered as the abundance of various microbes and denitrifying bacteria at the addition of FAC. This research showed that the presence of FAC is a useful strategy for the initiation of sludge particle formation to promote the treatment of wastewater, containing COD and NH4+ at about 150-100 and 30 mg L-1.

Biocathode regulates enrofloxacin degradation by coupling with different co-metabolism conditions.

In this study, biocathode system coupled with different co-metabolism conditions (NaAc, glucose and NaHCO3) were developed to degrade quinolones enrofloxacin (ENR) due to its poorly metabolization, easily accumulation and potential toxicity. Simultaneously, ENR reduction kinetic rate constant in NaAc-fed, glucose-fed and NaHCO3-fed biocathodes, and sole biocathode were increased by 343.62%, 320.46%, 189.19% and 130.88% when compared with that of abiotic cathode when the operational time and ENR concentration were set to 48 h and 25 mg/L. In addition, transformation pathways of ENR revealed pathway II were dominantly occurred in NaAc- and glucose-fed biocathode while pathway IV acting as key metabolic process were shown in NaHCO3-fed biocathode. Moreover, 16S rRNA high-throughput sequencing analysis indicated that biocathodic communities were sensitive to switch-over of carbon source, namely Delftia and Bosea as organohalide-respiring bacteria (OHRB) were abundant in NaAc- and glucose-fed biocathodes while Mesotoga and Syntrophorhabdus that responsible for benzoyl-CoA metabolic process were enriched in NaHCO3-fed biocathode. Overall, this study could unravel the underlying relationship between biocathode degradation pattern of ENR and different co-metabolism conditions, and further offer valuable scientific information on treating refractory quinolones antibiotics via green bioelectrochemical method.

Effect of different factors on treatment of oily wastewater by TiO2/Al2O3-PVDF ultrafiltration membrane.

An ultrafiltration membrane developed by our research group was applied to treat simulated emulsified oil wastewater. ATR-FTIR, SEM, TEM, and Zeta potential analyzes demonstrated that the modified ultrafiltration membrane (MM) has excellent stability and anti-fouling capacity than origin membrane (OM), which possesses a pure water flux of 260 L·m-2·h-1 and oil/water (o/w) rejection of 98.5 ± 0.33%. Inorganic salt CaCl2 has more considerable influence than MgSO4 and NaCl under the same mass concentration in the two membranes UF process. Along with concentration increasing, flux sharply reduces; meanwhile, the rejection has an opposite trend. Moreover, permeation flux has a maximum value, and the rejection also gets its optimal state under neutral conditions during the pH value of 2-12. The membrane also exhibits excellent anti-fouling performance and anti- o/w adsorption properties with an adsorption rate below 0.8% compared with OM, which has an adsorption rate of nearly 2.1%, respectively. A kind of new UF membrane developed by our research group was applied to treat simulated o/w. ATR-FTIR, SEM, TEM, and Zeta potential analyzes demonstrated that PVDF-Al2O3/TiO2 material has excellent stability and anti-fouling capacity. CaCl2 has the greatest influence than MgSO4 and NaCl under the same mass concentration. Moreover, permeation flux has maximum value and the rejection also gets its optimal state under neutral conditions during pH 2-12. The membrane also exhibits excellent anti-fouling performance and anti-O/W adsorption properties with adsorption rate below 0.8% compared with OM which has an adsorption rate nearly 2.1%, respectively.

Development of a Prognostic Model Based on the Identification of EMT-Related lncRNAs in Triple-Negative Breast Cancer.

BACKGROUND: Triple-negative breast cancer (TNBC) remains the most incurable subtype of breast cancer owing to high heterogeneity, aggressive nature, and lack of treatment options. It is generally acknowledged that epithelial-mesenchymal transition (EMT) is the key step in tumor metastasis. METHODS: With the application of TCGA and GEO databases, we identified EMT-related lncRNAs by the Cox univariate regression analysis. Optimum risk scores were calculated and used to divide TNBC patients into high-/low-risk subgroups by the median value using the Lasso regression analysis. The Kaplan-Meier and ROC curve analyses were applied for model validation. Then, we assessed the risk model from multi-omic aspects including immune infiltration, drug sensitivity, mutability spectrum, signaling pathways, and clinical indicators. We also analyzed the expression pattern of lncRNAs involved in the model using qRT-PCR in TNBC cell lines and constructed the ceRNA network. RESULTS: The risk model was composed of EMT-related long noncoding RNAs (lncRNAs), which seemed to be valuable in the prognostic prediction of TNBC patients. The model could act as an independent prognostic factor of TNBC and showed a robust prognostic ability in the stratification analysis. Further investigation demonstrated that the expression of lncRNAs was different between high aggressive and low aggressive TNBC cell lines, as well as TNBC patients. CONCLUSIONS: Together, our study successfully established a risk model with great accuracy and efficacy in the prognostic prediction of TNBC patients.

Simultaneously autotrophic denitrification and organics degradation in low-strength coal gasification wastewater (LSCGW) treatment via microelectrolysis-triggered Fe(II)/Fe(III) cycle.

The autotrophic iron-depended denitrification (AIDD), triggered by microelectrolysis, was established in the microelectrolysis-assistant up-flow anaerobic sludge blanket (MEA-UASB) with the purpose of low-strength coal gasification wastewater (LSCGW) treatment while control UASB operated in parallel. The results revealed that chemical oxygen demand (COD) removal efficiency and total nitrogen (TN) removal load at optimum current (2.5 A/m3) in MEA-UASB (83.2 ± 2.6% and 0.220 ± 0.010 kg N/m3·d) were 1.42-fold and 1.57-fold higher than those (58.5 ± 2.1% and 0.139 ± 0.011 kg N/m3·d) in UASB, verifying that AIDD and following dissimilatory iron reduction (DIR) process could offer the novel pathway to solve the electron donor-deficient and traditionally denitrification-infeasible problems. High-throughput 16S rRNA gene pyrosequencing shown that iron-oxidizing denitrifiers (Thiobacillus and Acidovorax species) and iron reducing bacteria (Geothrix and Ignavibacterium speices), acted as microbial iron cycle of contributors, were specially enriched at optimum operating condition. Additionally, the activities of microbial electron transfer chain, electron transporters (complex I, II, III and cytochrome c) and abundance of genes encoding important enzymes (narG, nirK/S, norB and nosZ) were remarkably promoted, suggesting that electron transport and consumption capacities were stimulated during denitrification process. This study could shed light on better understanding about microelectrolysis-triggered AIDD for treatment of refractory LSCGW and further widen its application potential in the future.

Ecological treatment technology for agricultural non-point source pollution in remote rural areas of China.

Agricultural non-point source pollution is one of the important reasons for rural water pollution, and it is also an important source of water eutrophication. In recent years, with the rapid economic growth and social changes in rural areas, large amounts of untreated domestic sewage and agricultural wastewater entering farmland require high efficiency, low operating costs, and minimal maintenance of treatment systems in rural and remote areas to minimize their impact on water and biodiversity. Since there is little research on the ecological treatment technology of agricultural non-point source pollution in China, from the perspective of controlling agricultural non-point source pollution, some ecological treatment technologies suitable for rural areas at home and abroad are summarized. This paper introduces the practical application of ecological treatment technology, the type of process, advantages and disadvantages, and the influencing factors of ecological treatment technology in the purification of sewage engineering and summarizes the removal mechanism of pollutants in ecological treatment technology. Eco-processing technologies are cost-effective in terms of their construction, maintenance, and energy needs and can be considered a sustainable wastewater treatment method, especially in remote areas and developing countries. It provides basic ideas for the construction of rural ecological treatment technology in China and puts forward suggestions and ideas for the future development trend of ecological treatment process sewage.

The underlying mechanism in gel formation and its mathematical simulation during anionic polyacrylamide solution ultrafiltration process.

A dead-end ultrafiltration cup was continuously operated to investigate the underlying mechanisms of membrane fouling caused by gel layer in this paper. Anionic polyacrylamide was used as a model foulant for gel formation process in various ultrafiltration processes by two kinds of ultrafiltration membrane, e.g., polyvinylidene fluoride (PVDF) membrane (OM) and TiO2/Al2O3-PVDF membrane (MM); then, a gel formation model was established and systematically assessed. The results show that the gel formation process in ultrafiltration can be divided into three stages: "slow-rapid-slow" flux decay curve. The R2 value of the simulation curve was still higher than 0.90 for both OM and MM. Based on the current cognition, the proposed gel layer formation mechanism and mathematical model were feasible.

Biological purification of acidic Fenton effluent by a fungal consortium without pre-neutralization upon base addition: Microbial screening and performance.

Great progresses have been made to carry out Fenton oxidation under neutral or alkaline pH in which, nevertheless, organic acids and other acidic intermediates usually result in acidic Fenton effluent. To eliminate the classical neutralization step prior to biological treatment, acid-tolerant microbes were here screened and used for purification of acidic Fenton effluent to achieve pH increase and further COD (chemical oxygen demand) removal. The bacterial and fungal community diversity was analyzed before and after screening for acid-tolerant microbes. After screening the bacterial diversity sharply decreased while the fungal diversity at the genus level became richer, mainly including Phialemoniopsis (relative abundance 38.69%), Vanrija (20.08%), Hypocreaceae (18.44%) and Candida (14.74%). Acidic pH and residual H2O2 are the features of Fenton effluent; hence, effects of pH and H2O2 on the screened acid-tolerant microbes were investigated in the aspects of growth rate and oxygen uptake rate. The kinetic parameters, including YH-biomass yield coefficient; Kd-biomass decay coefficient; µm-specific maximum COD removal rate; Ks-half saturation constant for COD removal, of the acid-tolerant microbes using 1/5 YM (yeast extract and malt extract culture medium) as substrate at 25 °C were measured by respirometric methodology. In BAC (biological activated carbon) inoculated with acid-tolerant microbes to treat actual Fenton effluent, the average COD removal efficiency was 72% at HRT (hydraulic retention time) of 3 h and the effluent pH was above 6 after removing the dissolved CO2 by air stripping. This study will provide a basis for developing a new combined process including Fenton and biological oxidation without pH adjustment.

Fate of antibiotics, antibiotic-resistant bacteria, and cell-free antibiotic-resistant genes in full-scale membrane bioreactor wastewater treatment plants.

The removal of antibiotics, antibiotic-resistant bacteria (ARB), and cell-free antibiotic-resistant genes (ARGs) and the microbial community of ARB were investigated in detail to understand their fate and provide valuable information on the feasibility of full-scale membrane bioreactor (MBR). The potential risks of cell-free ARGs to the receiving environment were discovered. High influent antibiotic concentration could inhibit the microbial activity of MBR sludge, whereas good antibiotic removal could be maintained because of relatively long solid retention time and high biomass retention. Approximately 61.8%-77.5% of the total antibiotics were degraded, and 22.5%-38.2% of the total antibiotics were adsorbed by MBR sludge on average. The individual antibiotic removal presented intense discrepancy because of the chemical construction and distribution coefficient of antibiotics. Aeromonas exhibited specific antibiotic resistance to ampicillin and erythromycin, Escherichia became the predominant genera in kanamycin-ARB and tetracycline-ARB, and Klebsiella and Bacteroides were the particular genera that exhibited distinct antibiotic resistance to ciprofloxacin. A significant correlation was found between cell-free ARG abundance and ARB content, and relatively high effluent cell-free ARG abundance facilitated the proliferation and transmission of ARB. The impacts of the receiving environment to eliminate the ecological risks and severe threats to human health should be investigated because of the low decay ratio and long-term persistence of cell-free ARGs.

Direct current triggering enhanced anaerobic treatment of acetyl pyrimidine-containing wastewater in up-flow anaerobic sludge blanket coupled with bioelectrocatalytic system.

In this study, the novel up-flow anaerobic sludge blanket coupled with bioelectrocatalytic system (UASB-BEC) was developed with the attempt to enhance treatment of acetyl pyrimidine-containing wastewater. The results revealed that higher current applied had a positive effect on acetyl pyrimidine (AP) degradation but a negative impact could be followed by the overhigh current (>1.26 A m-3). Removal efficiencies of AP and total organic carbon (TOC) were as high as 96.3 ±â€¯2.6% and 92.9 ±â€¯3.2% while methane production reached up to 0.70 ±â€¯0.03 NL-CH4 L-1-reactor d-1 at applied current of 1.26 A m-3, which were significantly higher those in control system. Moreover, high-throughput 16S rRNA gene pyrosequencing further indicated that Desulfovibrio and Methanimicrococcus species were specially enriched in suspended sludge and cathodic biofilm with current involvement. It could be reasonably speculated that enrichment of Desulfovibrio and Methanimicrococcus species could promote biotransformation of AP and final H2-depended methylotrophic methanogenesis. This study could shed light on better understanding of AP transformation in bioelectrocatalytic system and provide a valuable reference to practical application of anaerobic AP-containing wastewater treatment.

Reactivation and pilot-scale application of long-term storage denitrification biofilm based on flow cytometry.

The work provides a method on the basis of flow cytometry to evaluate the performance of denitrification biofilm during the preservation, reactivation and pilot-scale operation process. The viable cell ratio of denitrification biofilm significantly reduced and further led to the decrease of denitrification capacity after long-term preservation for 5 months. Protein component in tightly bound extracellular polymeric substances (TB-EPS) could serve to enhance microbial adhesion and promote denitrification biofilm formation. With the significant correlation of viable cell ratio and microbial characteristics, 4 °C was more appropriate for preserving denitrification biofilm and conducive to maintain the relatively high denitrification capacity. A maximum denitrification rate of 5.80 gNO3--N/m2·d was obtained in pilot-scale anoxic-oxic (AO) process and Dechloromonas became greater prevalence in denitrification suspended carriers. Furthermore, the enrichment of Pseudomonas, Parcubacteria, Acidovorax, Aquabacterium and Unclassified_Flavobacteriaceae enhanced biofilm formation and nutrient conservation. The significantly positive correlation between viable cell ratio and the ratio of nitrate reduction to COD consumption was discovered, and the indices of Chao, ACE, Shannon and Simpson of denitrification biofilm were positively correlated with viable cell ratio, meaning that flow cytometry analysis was reasonable and suitable to evaluate the performances of denitrification biofilm.

Adsorption Mechanism of Oil-in-Water on a TiO2/Al2O3-Polyvinylidene Fluoride (PVDF) Ultrafiltration Membrane.

For the sake of gaining a clear idea of the adsorption mechanism involved with an oil emulsion-membrane system, Daqing crude oil emulsion and two types of polyvinylidene fluoride (PVDF) ultrafiltration membranes made in our laboratory were used as the objects to pursue the adsorption characteristics in this system. Several isotherm and kinetics models were used here to simulate the adsorption process; the effect of variables such as time, initial concentration, temperature, as well as scanning electron microscopy (SEM) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectra were investigated to assist in understanding the mechanism. The results show that the Redlich-Peterson model and the pseudo-first-order kinetic model are the best fitting models, with all of the models exhibiting correlation coefficient ( R2) values of >0.98, suggesting an endothermic adsorption process that involves a combination of chemical and physical mechanisms. Moreover, the thermodynamic parameters, such as Δr Gmθ, Δr Hmθ, and Δr Smθ were also calculated from the temperature dependence, indicating a nonspontaneous process, and increases in temperature had a negative effect on the oil-in-water (o/w) adsorption. Ultimately, further evidence is obtained from the microstructural and infrared spectral analyses.

Critical flux investigation in treating o/w emulsion by TiO2/Al2O3-PVDF UF membrane.

A standard transmembrane pressure (TMP) step method has been used in membrane fouling assessment in tube ultrafiltration (UF) membrane system treating oil water (o/w) emulsion operated at constant TMP. Three flux reduction curve with different o/w concentration based on TMP variation were concluded by experiment, then, to describe fouling behavior and identify the occurrence of fouling in the so-called critical flux. Furthermore, sub-critical and super-critical flux experiment with a long time was determined, and zero rate of flux reduction (dF/dt) was never found during the whole trial period, indicating that critical flux in o/w UF process with its strict definition could not be defined in this paper. However, quasi-critical flux exists, under which the pollution rate was very slow. Moreover, a high-efficiency four steps cleaning method: mechanic scraping, pure water wash, pure water reverse wash, and dosing cleaning, was explored. It concluded that critical flux in real o/w UF system determined by TMP-step method can be used to predict long-term critical behavior with useful data on fouling propensity.