Robustness of Latent Profile Analysis to Measurement Noninvariance Between Profiles.

Latent profile analysis (LPA) identifies heterogeneous subgroups based on continuous indicators that represent different dimensions. It is a common practice to measure each dimension using items, create composite or factor scores for each dimension, and use these scores as indicators of profiles in LPA. In this case, measurement models for dimensions are not included and potential noninvariance across latent profiles is not modeled in LPA. This simulation study examined the robustness of LPA in terms of class enumeration and parameter recovery when the noninvariance was unmodeled by using composite or factor scores as profile indicators. Results showed that correct class enumeration rates of LPA were relatively high with small degree of noninvariance, large class separation, large sample size, and equal proportions. Severe bias in profile indicator mean difference was observed with intercept and loading noninvariance, respectively. Implications for applied researchers are discussed.

Comparing Chinese youth with American youth on adjustment: An illustration of measurement issues in cross-cultural research.

In this article, we used results from two studies to show the need to go beyond linguistic equivalence to establish construct validity and measurement invariance in cross-cultural research. Study 1 examined Rosenberg Self-Esteem (RSE; 10 items) data from 156 Mainland Chinese youth (M = 13.8 years, SD = .53) and 213 Chinese-American youth (M = 13.6 years, SD = 2.1) from high socioeconomic status (SES) families. The Chinese translation of the RSE has been widely used. Study 2 included 1060 Mainland Chinese youth (M = 15.6 years, SD = 2.3) and 412 racially diverse American youth (M = 16.0, SD = 2.9) from all SES backgrounds. Data were collected with the third and newest edition of the Behavioural Assessment System for Children-Self Report of Personality (BASC-3-SRP; 189 items). We translated and back-translated the BASC-3-SRP between English and Chinese to establish linguistic equivalence. All participants were females. Study 1 showed that the RSE had acceptable internal consistency but lacked construct validity. Study 2 showed that the original and the translated BASC-3-SRP had good internal consistency and construct validity, but nine of its 16 subscales lacked measurement invariance. These results highlight measurement issues facing international and cross-cultural research.

[CircRNA_005647 inhibits expressions of fibrosis-related genes in mouse cardiac fibroblasts via sponging miR-27b-3p].

OBJECTIVE: To investigate the effect of circRNA_005647 on fibrotic phenotype of mouse cardiac fibroblasts (CFs) and explore its mechanism. METHODS: We used an angiotensin Ⅱ (Ang-Ⅱ) capsule pump (daily dose of 1.46 mg/kg for 2 weeks) to establish a mouse model of myocardial fibrosis in C57BL/6 mice. Masson staining was used to detect myocardial fibrosis in the myocardium. The expression of circRNA_005647 in the myocardium of Ang-Ⅱ-infused mice and in Ang-Ⅱ-treated CFs were detected with real-time PCR. Actinomycin D and RNase R exonuclease digestion were used to test the stability of circRNA_005647 in mouse CFs. Over- expression of circRNA_005647 was achieved in the CFs by infecting the cells with a recombinant circRNA_005647 adenovirus (rAd-circRNA_005647), and the expressions of Col1a1, Col3a1 and Acta2 were detected in the cells with real-time PCR and Western blotting. Dual luciferase reporter assay, RNA antisense purification and RNA Pull down assay were performed to identify the interaction between circRNA_005647 and miR-27b-3p. RESULTS: CircRNA_005647 was up- regulated in the myocardium of Ang-Ⅱ- infused mice and in Ang-Ⅱ-treated mouse CFs (P < 0.01). CircRNA_005647 was more stable than its host gene Myosin IXA (Myo9a) in response to actinomycin D (P < 0.01) and RNase R exonuclease treatment. The expressions of fibrosis-associated genes was down-regulated in the CFs over-expressing circRNA_ 005647 (P < 0.05). Dual luciferase reporter assay, RNA antisense purification and RNA Pull down assay revealed the interaction between miR-27b-3p and circRNA_005647. MiR-27b-3p obviously enhanced the fibrotic phenotype but reversed the inhibitory effect of circRNA_005647 on the expression of fibrosis associated genes in the CFs (P < 0.05). CONCLUSIONS: CircRNA_005647 is upregulated in cardiac fibrosis and inhibits the expression of fibrosis-related genes through sponging miR-27b-3p in mouse CFs.