MiR-17-5p Inhibits the Proliferation and Metastasis of Gastric Cancer Cells by Targeting PTEN Protein.

Context: Effectively inhibiting gastric-cancer metastasis and decreasing the recurrence of gastric cancer after surgery are urgent problems. In recent years, abnormal microRNA (miRNA) expression has been found in gastric cancer, and miRNA-17-5p has been found to regulate the occurrence and progression of various cancers. It's necessary to analyze miRNA-17-5p's action mechanism in gastric cancer. Objective: The study intended to investigate: (1) miR-17-5p expression in gastric-cancer tissues and adjacent normal tissues in clinical patients, (2) to explore the regulatory effects of miR-17-5p on the biological behavior of gastric cancer at the cellular level, by constructing a gastric-cancer cell model that uses overexpressing miR-17-5p, and (3) to discuss preliminarily its action mechanism. Design: The research team designed a laboratory study. Setting: The study took place at the First Affiliated Hospital of Jinzhou Medical University in Jinzhou, Liaoning Province, China. Participants: Clinical specimens of gastric-cancer tissues and adjacent normal tissues were obtained from 20 patients who had undergone surgical resection for gastric cancer at the hospital. Outcome Measures: Quantitative reverse transcription (qRT)-polymerase chain reaction (PCR) was employed to detect miR-17-5p expression in the gastric-cancer tissues and cells. The si-miR-17-5p was transfected into gastric-cancer cell lines to establish a cell model, with the si-miR-17-5p becoming the intervention group and a negative control group, the si-NC group also being created. The cultured SGC-7901 cells were divided into miR-17-5p mimic group (si-miR-17-5p) and negative control group (si-NC) by transfecting si-miR-17-5p and transfection reagent Lipo2000 for research. Cell proliferation was detected using the cell counting kit-8 (CCK-8) assay; cell invasion and migration were measured using a Transwell assay; and cell migration was also measured using a wound-healing assay. A bioinformatics prediction, luciferase reporter gene assay, and western blot assay were applied to verify the downstream target protein of miR-17-5p. Results: The miR-17-5p levels were significantly higher in gastric-cancer tissues and cell lines than in adjacent normal tissues or gastric epithelial cells. Gastric-cancer cells that were transfected with si-miR-17-5p were found to have significantly inhibited cell proliferation. The si-miR-17-5p could significantly suppress the invasion and metastasis of gastric-cancer cells. A bioinformatics prediction concluded that phosphatase and tensin homolog (PTEN) was the downstream target protein of miR-17-5p, which was confirmed by the Luciferase reporter gene assay and Western blot assay. Conclusions: The miR-17-5p was upregulated in gastric-cancer patients. Simultaneously, si-miR-17-5p dramatically inhibited the proliferation, invasion, and metastasis of gastric-cancer cells, and PTEN protein was its downstream target protein. The study provides a new approach to the treatment of gastric cancer.

The progress in diagnosis and treatment of oligometastatic prostate cancer / 中华泌尿外科杂志

Previous studies believe that oligometastasis has unique biological characteristics. Early active treatment for patients with oligometastatic prostate cancer can delay disease progression and improve survival. However, the current definition of oligometastasis is still unclear, and its optimal treatment is still a major concern of the medical community. This article reviewed recent research progresses in term of the definition and comprehensive treatment strategy of oligometastatic prostate cancer.

Noninvasive evaluation of esophageal and gastric varices in liver cirrhosis by shear-wave ultrasound elastography / 临床肝胆病杂志

Esophagogastric variceal bleeding is one of the main causes of death in patients with liver cirrhosis, and therefore, early monitoring of esophageal and gastric varices may help to improve the prognosis of patients; however at present, hepatic venous pressure gradient and upper gastrointestinal endoscopy used as the gold standard for diagnosis are invasive examinations, which may not help with long-term follow-up. This article introduces the noninvasive evaluation of esophageal and gastric varices in cirrhotic patients by shear-wave ultrasound elastography.

Precision of digital impressions with TRIOS under simulated intraoral impression taking conditions / 北京大学学报(医学版)

Objective:To evaluate the precision of digital impressions taken under simulated clinical impression taking conditions with TRIOS and to compare with the precision of extraoral digitalizations . Methods:Six #14 -#17 epoxy resin dentitions with extracted #16 tooth preparations embedded were made.For each artificial dentition , ( 1 ) a silicone rubber impression was taken with individual tray , poured with type IV plaster ,and digitalized with 3Shape D700 model scanner for 10 times;(2) fastened to a dental simulator , 10 digital impressions for each were taken with 3Shape TRIOS intraoral scanner .To assess the precision , best-fit algorithm and 3 D comparison were conducted between repeated scan models pairwise by Geomagic Qualify 12.0, exported as averaged errors (AE) and color-coded diagrams.Non-parametric analysis was performed to compare the precisions of digital impressions and model images .The color-coded diagrams were used to show the deviations distributions .Results:The mean of AE for digital impressions was 7.058 281 μm, which was greater than that of 4.092 363 μm for the model images (P<0.05).However, the means and medians of AE for digital impressions were no more than 10 μm, which meant that the consistency between the digital impressions was good .The deviations distribution was uniform in the model images ,while nonuniform in the digital impressions with greater deviations lay mainly around the shoulders and interproximal surfaces .Conclusion:Digital impressions with TRIOS are of good precision and up to the clinical standard .Shoulders and interproximal surfaces scanning are more difficult.

The correlation of 18F-fluoroestradiol uptake in patients with breast cancer to in vitro immunohistochemical assay of ER status / 中国癌症杂志

Background and purpose:16α-[18F]lfuoroestradiol (18F-FES) is an in vivo speciifc imaging agent for estrogen receptor (ER). We investigated the concordance between tumor ER status as determined by FES-PET and in vitro immunohistochemical assays. Methods: 18F-FES was prepared by ourselves. Twenty-six patients were enrolled (17 primary and 9 metastatic/recurrent). Patients underwent both 18F-FES and 18F-FDG PET/CT. Results:We found good overall agreement (96.15%) between in vitro ER assays and FES-PET. The ER status diagnosis sensitivity of 18F-FES was 93.33%and the speciifcity was 100%when using cut-off value of SUVmax≥1.5. There was a positive correlation between in vitro ER, PR assays and the SUVmax of 18F-FES while in vitro HER-2/neu assays correlatived negatively with 18F-FES SUVmax. Conclusion:These results suggested 18F-FES may be useful for studying the ER expression of all malignant lesions in patients with breast cancer and guiding individual therapy.