Neurotoxic and immunotoxic effects of Indole-3-butyric acid on rats at subacute and subchronic exposure.

This study was carried out to investigate the neurotoxic and immunotoxic effects of Indole-3-butyric acid (IBA), a plant growth regulator (PGR), on rats at subacute and subchronic exposure. The neurotoxic effects of IBA were evaluated by measuring the activities of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Biomarkers selected for immunotoxic monitoring were the activities of adenosine deaminase (ADA) and myeloperoxidase (MPO) in various tissues of rats exposed to 25 and 50 ppm dosages of IBA for 20 and 45 days. Results showed that the administrations of IBA decreased AChE and BChE activities in some tissues of the rats treated with both dosages and periods of IBA. With regard to the immunotoxic effects, ADA activity significantly decreased whereas MPO activity increased after subacute and subchronic exposure with both dosages in most of the tissues of rats compared with controls. The observations presented led us to conclude that the administrations of IBA at subacute and subchronic exposure decreased AChE, BChE and ADA activities whereas increased MPO activity in various tissues of rats. This may reflect the potential role of these parameters as useful biomarkers for toxicity of IBA.

Hematotoxic and hepatotoxic effects of dichlorvos at sublethal dosages in rats.

The present study was designed to understand the effects of sublethal concentrations of dichlorvos (DIC) on hematological constituent [red blood corpuscles, white blood corpuscles (WBC), mean cell volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, platelet counts, hemoglobin and hematocrite levels] and serum damage marker enzymes (aspartate aminotransferase, alanin aminotransferase, alkaline phosphatase, and lactate dehydrogenase) in rats at subacute period under laboratory conditions. DIC at dosages of 5 and 10 ppm was administered orally to six male rats ad libitum during the tests for 4 weeks consecutively. According to the results, DIC treatments increased significantly the levels of serum marker enzyme activities, whereas they did not change hematologic constituent except for WBC number treated with both dosages of DIC. The observations presented led us to conclude that the administrations of subacute DIC induced the levels of damage marker enzymes and leukocytosis.