Nest box entrance hole size can influence nest site selection and nest defence behaviour in Japanese tits.

Birds have the ability to assess the risk of predation in their environment and adjust their antipredation strategies based on this risk information. However, whether nest site selection has effect on subsequent nest defence behaviour has not been studied. In this study, we aimed to determine whether the Japanese tit (Parus minor) exhibits a nest-box hole size preference and whether the entrance hole sizes of nest boxes influence the nest defence behaviour of tits. We hung nest boxes with three different entrance hole sizes (diameters: 6.5 cm, 4.5 cm and 2.8 cm) in our study sites and investigated which nest boxes were occupied by tits. In addition, by using dummy-presentation experiments, we observed the nest defence behaviours of tits that nested in boxes with 2.8 cm and 4.5 cm entrance holes towards common chipmunks (Tamias sibiricus, a small nest predator able to enter these holes) and Eurasian red squirrels (Sciurus vulgaris, a large nest predator unable to enter the 2.8 cm entrance hole). The tits that bred in nest boxes with 2.8 cm entrance holes exhibited more intense nest defence responses to chipmunks than to squirrels. In contrast, the tits that bred in nest boxes with 4.5 cm entrance holes exhibited similar nest defence responses to chipmunks and squirrels. Additionally, Japanese tits that bred in nest boxes with 2.8 cm entrance holes exhibited more intense behavioural responses to chipmunks than those that bred in nest boxes with 4.5 cm entrance holes. Our results suggested that Japanese tits prefer to occupy nest boxes with small holes for breeding and that nest-box characteristics influenced their nest defence behaviour.

A case report of an Eurasian jay (Garrulus glandarius) attacking an incubating adult and depredating the eggs of the Japanese tit (Parus minor).

In May 2021, we opportunistically observed one Eurasian jay (Garrulus glandarius) attacking an adult incubating Japanese tit (Parus minor) and depredating nine tit eggs at a nest box where a woodpecker had greatly enlarged the entrance. After the predation event, the Japanese tits abandoned the nest. We recommend that when using artificial nest boxes to protect hole-nesting birds, the appropriate entrance size should be proportional to the body size of the target species. This observation gives us a better understanding of the potential predators of secondary hole-nesting birds.

Geographical variation in nest defense among cinereous tit populations in China.

Behavioral divergence among populations is common across taxonomic groups, still we know very little about anti-predator behaviors. Animal exposure to predation risk is variable in different ecological contexts. In addition, reproduction value of animals in different geographical regions usually varies. In this study, we tested whether cinereous tits Parus cinereus in different populations exhibited nest defense behaviors similar to those of nest or adult predators and whether their nest defense behaviors showed geographical variation. By using field dummy experiments, we observed tits' nest defense behavior in nest predator common chipmunk Tamias sibiricus and red squirrel Sciurus vulgaris, adult predator Eurasian sparrowhawk Accipiter nisus and nonthreatening species Oriental turtle dove Streptopelia orientalis in the ZJ (44° N), DZ (31° N), and DLS (18° N) populations, respectively. The response scores varied significantly across the 4 dummies in ZJ-tits and DLS-tits but did not in DZ-tits. When facing the chipmunk, ZJ-tits showed the highest response score and DZ-tits showed the lowest response score. When facing the squirrel, ZJ-tits showed a higher response score than tits in the other 2 populations. However, tits among the 3 populations responded similarly to a sparrowhawk or dove with slight response behaviors. In addition, response scores to nest predators were positively correlated with brood size across the 3 populations, but no trend was found for sparrowhawks or doves. Our results indicated that the nest defense behaviors of cinereous tits have evolved in response to large-scale geographical variation in ecological contexts and reproduction value.

Geographic Variation in Note Types of Alarm Calls in Japanese Tits (Parus minor).

Geographic variability in acoustic signals has been documented in many bird species. However, geographic variations in alarm calls have been so far neglected despite their crucial role on reducing risk to group members and relatives. We analyzed the note types and acoustic parameters of Japanese tit (Parus minor) alarm calls to three types of intruders (a nest predator, an adult predator, and a harmless species) from three populations in China. Our results revealed that tits in the same population produce similar note types to different intruders, but the three populations only shared six note types and each population had unique note types. The frequency and duration parameters of three shared common note types were significantly different among populations. The three populations belong to the same species, thus they have shared note types. We suspect that the unique note types occurring in each population may be related to three potential reasons: founder effect, predation pressure, and vocal learning. The differences in acoustic parameters of common notes among populations may be a consequence of adaptations to their environments. We suggest that population differences in the note levels of bird alarm calls do exist.