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1.
ACS Omega ; 9(17): 18973-18983, 2024 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-38708250

RESUMO

In coal pillar fires, the fire source is hard to be detected and the adjacent goaf is extremely likely to be affected. Such fires would give rise to thermal and dynamic disasters in mines, further causing casualties and environmental disruption. In this study, with the coal pillar spontaneous combustion (CPSC) accident in Chahasu Coal Mine taken as the research object, the oxygen uptake and limit of oxygen concentration of CPSC were explored. Based on the research results, a similar model was constructed, where a control group was used to simulate the hazardous area of CPSC in a bid to investigate the law of CPSC. Moreover, the polymer colloid perfusion system was constructed and the drilling parameters and perfusion process parameters were determined, and practices concerning spontaneous fire control were carried out. Here are the conclusions: First, air leakage in the coal pillar may lead to coal spontaneous combustion due to the impact of rib spalling, threatening 2-6 m above the middle of the coal pillar at a shallow position. Second, coal pillar grouting, injecting polymer colloids for cooling, and coal pillar cement reinforcement prove to be effective ways to prevent CPSC fires and combat recombustion.

2.
Bioresour Technol ; 190: 381-7, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25974352

RESUMO

In this work, spent mushroom substrates were utilized for screening a microbial consortium with highly simultaneous degradation of lignocellulose and chlorophenols. The desired microbial consortium OEM1 was gained through successive cultivation for about 50 generations and its stability of composition was verified by denaturing gradient gel electrophoresis (DGGE) during screening process. It could degrade lignocellulose and chlorophenols at around 50% and 100%, respectively, within 7days. The diversity analysis and the growth characteristics of OEM1 during degradation process were investigated by PCR-DGGE combined with clone and sequence. The results indicated that OEM1 consisted of 31 strains. Proteobacteria and Bacteroidetes were the predominant bacterial groups. The dynamic change of OEM1 illustrated that consortium community structure was effected by pH and substrate alteration and tended to be stable after 6days' cultivation. Furthermore, bacteria (11 strains) and actinomycetes (2 strains) were obtained based on plate isolation and identified via 16S rDNA sequence.


Assuntos
Agaricales/isolamento & purificação , Bactérias/isolamento & purificação , Bactérias/metabolismo , Clorofenóis/metabolismo , Lignina/metabolismo , Consórcios Microbianos/fisiologia , Bactérias/classificação , Técnicas de Tipagem Bacteriana , Biodegradação Ambiental , Resíduos Industriais/prevenção & controle , Especificidade da Espécie
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