RESUMO
OBJECTIVE: To investigate the expression of A-type atrial natriuretic peptide receptor (ANPR-A) in the kidneys of renovascular hypertension rats and evaluate the significance of the expression. METHODS: The rat model of renovascular hypertension was produced by constricting one lateral renal artery. After the renal artery being constricted for 4 weeks and 8 weeks, the systolic BP of rats was measured with a manometer using the tail-cuff method. Then, the expression of ANPR-A was respectively detected by immunohistochemical technique in the kidneys of the two-kidney, one-clip (2K1C) rats, and the expression level of ANPR-A was semi-quantitatively measured by Mias-2000 computer image analyzer. RESULTS: At 4 weeks after the artery-constricted operation,the expression of ANPR-A increased significantly in 2K1C hypertensive rat glomeruli and decreased significantly in renal tubules, compared with control (P<0.01), but there was no marked change in medullar collecting tubules. At 8 weeks after the artery-constricted operation, the expression of ANPR-A decreased significantly in 2K1C hypertensive rat renal tubules and medullar collecting tubules, compared with control (P<0.01); however, there was weak expression in glomeruli, and no statistically significant difference was seen when compared with control (P>0.05). CONCLUSION: The expression of ANPR-A decreased significantly in kidney tissues of renovascular
Assuntos
Hipertensão Renovascular/metabolismo , Rim/metabolismo , Receptores do Fator Natriurético Atrial/biossíntese , Animais , Fator Natriurético Atrial/metabolismo , Hipertensão Renovascular/etiologia , Masculino , Ratos , Ratos Sprague-Dawley , Receptores do Fator Natriurético Atrial/genéticaRESUMO
OBJECTIVE: To investigate the role of circadian gene mPeriod2 (mPer2) on tumor proliferation and apoptosis. METHOD: The eukaryotic mPer2 expression vector (pcDNA 3.1-mPer2) based on pcDNA 3.1 was transfected into the cultured Lewis tumor cell by liposome method in vivo. The expression of mPer2 in transfected Lewis tumor cell was detected by immunohistochemistry and flowcytometry. The stable transfected cell line was screened by G418 and its proliferation and apoptosis was examined by flowcytometry. RESULT: Expression of PERIOD2 protein in pcDNA 3.1-mPer2 transfected Lewis tumor cell was proved by immunohistochemistry and flowcytometry. Flowcytometry result of mPer2 expression Lewis tumor cell showed less proliferation and high rate of apoptosis. CONCLUSION: mPer2 expression can suppress the proliferation of tumor cell and increase the apoptosis of it.
Assuntos
Ritmo Circadiano/genética , Proteínas Nucleares/metabolismo , Fatores de Transcrição/metabolismo , Animais , Apoptose , Carcinoma Pulmonar de Lewis/genética , Proteínas de Ciclo Celular , Divisão Celular , DNA Complementar/genética , Citometria de Fluxo , Expressão Gênica , Camundongos , Proteínas Nucleares/genética , Proteínas Circadianas Period , Fatores de Transcrição/genética , Transfecção , Células Tumorais Cultivadas/imunologiaRESUMO
OBJECTIVE: To investigate the influence of Shenfu injection on the hemodynamic indexes of myocardial ischemic dogs and blood pressure of dogs and rats. METHOD: Myocardial ischemic model was made and hypotension in the dogs was induced with ligating left front descending limb coronary artery method, and secondary hypertension by narrowing nephridium artery of rats, Shenfu injection was administered with 5, 10 mL.kg-1 to the above dogs and rats separately to investigate the influence of it on the hemodynamic indexes of myocardial ischemic dogs and blood pressure of rats. RESULT AND CONCLUSION: Shenfu injection enhanced the capacity of myocardial work markedly; it augmented the myocardium contractility and cardiac output without raising the oxygen consume, and at the same time it returned normal the blood pressure in myocardial ischemic; but it had no effects on the normal blood pressure and secondary hypertension of rats.
